食品与发酵工业 >

2025 , Vol. 51 >Issue 14: 210 - 217

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041892

研究报告

溶解氧调控酿酒污水再利用效果及微生物机制

  • 柏松 ,
  • 杨英 ,
  • 何静 ,
  • 罗玲 ,
  • 杜娟 ,
  • 徐丽萍
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  • 1(四川化工职业技术学院 川酒学院,四川 泸州,646000)
    2(天然产物与功能性食品泸州市重点实验室,四川 泸州,646000)
第一作者: 学士,讲师(徐丽萍教授为通信作者,E-mail:270177422@qq.com)

收稿日期: 2024-12-18

  修回日期: 2025-03-04

  网络出版日期: 2025-08-01

基金资助

四川省重点研发计划项目(2023YFN0079)泸州市应用基础研究科技项目(2023JYJ009)

收起

Regulation of dissolved oxygen for brewing wastewater reuse and its microbial mechanisms

  • BAI Song ,
  • YANG Ying ,
  • HE Jing ,
  • LUO Ling ,
  • DU Juan ,
  • XU Liping
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  • 1(Chuanjiu College, Sichuan Vocational College of Chemical Technology, Luzhou 646000, China)
    2(Luzhou Key Laboratory of Natural Products and Functional Food Development, Luzhou 646000, China)

Received date: 2024-12-18

  Revised date: 2025-03-04

  Online published: 2025-08-01

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摘要

酿酒过程中会产生大量污水,有机物浓度高、处理难度大。同时,生活污水的低碳化导致其缺乏有机物供给、氮素去除效果差。该研究采用酿酒污水作为碳源强化生活污水脱氮,考察了不同溶解氧(dissolved oxygen,DO)条件下的污水脱氮效果、微生物群落结构、基因富集特征,并设计了应用模式。结果表明,DO 3~5 mg/L条件下,酿酒污水与生活污水按照体积比1∶380混合处理,可以满足GB 18918—2002《城镇污水处理厂污染物排放标准(含修改单)》一级A标。DO浓度降低将导致菌属数量减少,通过影响Sphaerotilus、Hydrogenophaga、Zoogloea菌属协同演替,从而调控污染物去除性能。此外,氮代谢途径中amoA、amoB、amoC、nirK、nosZ是容易受影响的关键基因,其丰度降低将直接削减污染物去除率。将酿酒污水用作城镇生活污水处理厂或者酿酒厂自身生活污水处理中的碳源,均可实现降本增效、节能降碳。该研究为酿酒污水再利用技术提供了借鉴。

关键词： 酿酒污水; 生活污水; 溶解氧; 脱氮性能; 微生物群落结构

本文引用格式

柏松 , 杨英 , 何静 , 罗玲 , 杜娟 , 徐丽萍 . 溶解氧调控酿酒污水再利用效果及微生物机制[J]. 食品与发酵工业, 2025 , 51(14) : 210 -217 . DOI: 10.13995/j.cnki.11-1802/ts.041892

Abstract

During the brewing process, a large amount of wastewater is generated, characterized by high concentrations of organics and significant treatment challenges.Meanwhile, the low carbon content in domestic wastewater leads to a deficiency in organic matter supply and poor nitrogen removal efficacy.This study utilized brewing wastewater as a carbon source to enhance nitrogen removal from domestic wastewater.It investigated nitrogen removal efficiency, microbial community structure, and gene enrichment characteristics under different dissolved oxygen (DO) conditions and designs an application model.Results indicated that under DO conditions of 3-5 mg/L, mixing brewing wastewater with domestic wastewater at a volume ratio of 1∶380 could meet the level 1A standard of GB 18918—2002.A decrease in DO concentration led to a reduction in microbial diversity, influencing the synergistic succession of genera such as Sphaerotilus, Hydrogenophaga, and Zoogloea, thereby regulating pollutant removal performance.Additionally, the key genes amoA, amoB, amoC, nirK, and nosZ in nitrogen metabolism pathways were more susceptible to such changes, diminishing pollutant removal performance.Reusing brewing wastewater in municipal domestic wastewater treatment plants or within the own domestic wastewater treatment facilities of breweries as a carbon source could achieve cost reduction, efficiency enhancement, energy conservation, and carbon reduction.This study provides valuable insights into brewing wastewater reuse technologies.

Key words： brewing wastewater; low carbon wastewater; dissolved oxygen; denitrification performance; microbial community structure

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