益生菌的功能特性与安全性评价是其应用开发的重要基础。该研究聚焦长双歧杆菌婴儿亚种CICC 6069T,对其基因组特征、安全性、益生特性进行了系统研究,并且建立了其菌株水平的快速鉴定方法。全基因组分析揭示了该菌株丰富的碳水化合物和氨基酸代谢基因,以及抗氧化、母乳低聚糖(human milk oligosaccharides,HMOs)代谢、植酸代谢相关基因,展现出作为功能益生菌的巨大潜力。安全性评价表明,该菌株对常规抗生素敏感,无溶血活性,无急性毒性,可以作为益生菌安全使用。益生特性评价实验表明,该菌株有良好的黏附性、DPPH自由基清除能力、较强的植酸酶活力,能够有效促进肠道细胞对于铁元素的吸收,结合基因分析和体外实验验证了该菌株在促进矿物元素吸收的潜力。基于脂蛋白编码基因6069GL001191设计特异性引物,建立了高效的CICC 6069T菌株水平鉴定方法,为实现其知识产权保护和质量控制提供先进的技术支持。该研究从功能、安全性和知识产权保护等多个角度进行全方位探讨,为CICC 6069T在肠道健康调控领域的应用提供坚实的理论支撑,助力其在益生菌产业中的创新发展。
Assessing the functional characteristics and safety of probiotics is essential for their application.This study systematically investigated the genomic features, safety, and probiotic properties of Bifidobacterium longum subsp.infantis CICC 6069T and established a rapid strain-level identification method.Whole-genome analysis revealed abundant genes related to carbohydrate and amino acid metabolism, as well as those associated with antioxidant activity, human milk oligosaccharides (HMOs) metabolism, and phytic acid degradation, highlighting its potential as a functional probiotic.Safety evaluation showed that this strain is sensitive to common antibiotics, exhibits no hemolytic activity, and has no acute toxicity, confirming its suitability for probiotic use.Probiotic property assessments demonstrated strong adhesion ability, high DPPH free radical scavenging activity, and significant phytase activity.It effectively promoted iron absorption in intestinal cells, with genomic and in vitro analyses supporting its potential for enhancing mineral uptake.A strain-specific identification method was developed using primers targeting the lipoprotein-encoding gene 6069GL001191, providing an advanced tool for intellectual property protection and quality control.This study comprehensively explored the functionality, safety, and industrial applications of the strain, offering a solid theoretical foundation for its use in gut health regulation and innovation in the probiotic industry.
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