食品与发酵工业 >

2025 , Vol. 51 >Issue 14: 336 - 342

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041207

分析与检测

CdTe量子点单克隆抗体电化学发光检测黄曲霉毒素B1的方法研究

  • 唐璎 ,
  • 周晓芳 ,
  • 马婷婷
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  • (甘肃省产品质量监督检验研究院,甘肃 兰州,730050)
第一作者: 硕士,高级工程师(通信作者,E-mail:tangying8642@163.com)

收稿日期: 2024-10-05

  修回日期: 2024-12-02

  网络出版日期: 2025-08-01

基金资助

国家市场监督管理总局科技项目(2023MK119)陇原青年创新创业人才项目(2024QNTD15)

收起

Study on electrochemiluminescence detection method of aflatoxin B1 using CdTe quantum dot monoclonal antibody

  • TANG Ying ,
  • ZHOU Xiaofang ,
  • MA Tingting
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  • (Gansu Province Product Quality Supervision and Inspection Research Institute, Lanzhou 730050, China)

Received date: 2024-10-05

  Revised date: 2024-12-02

  Online published: 2025-08-01

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摘要

该研究设计了一种结合AFB1单克隆抗体与CdTe量子点材料的电化学传感器,建立了量子点单克隆抗体(quantum dot monoclonal antibody,QDs-mAb)用于AFB1的可视化快速定量筛查检测方法。当AFB1抗原与AFB1偶联的比例为50∶1时,QDs-mAb法的检测范围为0.03～12 μg/L,最低检测限(limit of detection,LOD)为0.03 μg/L。该方法具有较高的灵敏度、宽广的线性范围,回收率为84.62%～114.47%,并且与现行国家标准GB 5009.22—2016《食品安全国家标准 食品中黄曲霉毒素B族和G族的测定》检测结果表现出高度一致性。该研究建立的QDs-mAb方法实现了食品和粮食中AFB1的现场即时检测,旨在提升基层监管机构的日常筛查检测水平。

关键词： 黄曲霉毒素B1; 抗原; 单克隆抗体; 量子点; 电化学发光

本文引用格式

唐璎 , 周晓芳 , 马婷婷 . CdTe量子点单克隆抗体电化学发光检测黄曲霉毒素B1的方法研究[J]. 食品与发酵工业, 2025 , 51(14) : 336 -342 . DOI: 10.13995/j.cnki.11-1802/ts.041207

Abstract

This study designed an electrochemical sensor combining AFB1 monoclonal antibodies with CdTe quantum dots, establishing a new method for visual rapid quantitative screening of AFB1 using quantum dot monoclonal antibody (QBS-mAb).When the coupling ratio of AFB1 antigen to AFB1was 50∶1, the QBS-mAb method exhibited a detection range of 0.03-12 μg/L, with a limit of detection (LOD) of 0.03 μg/L.The method demonstrated high sensitivity, a wide linear range, and recovery rates between 84.62% and }, showing strong consistency with the national standard GB 5009.22—2016.The developed QBS-mAb method enabled point-of-care testing for food and grain, aiming to enhance routine screening detection capabilities at grassroots regulatory agencies.

Key words： aflatoxin B1; antigen; monoclonal antibody; quantum dot; electrochemiluminescence

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