分析与检测

基于表面增强拉曼光谱法快速筛查液体保健食品中90种非法添加磷酸二酯酶5型抑制剂研究

  • 李玉静 ,
  • 何洪源 ,
  • 栾玉静
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  • 1(中国人民公安大学 侦查学院,北京,100038)
    2(公安部鉴定中心,北京,100038)
第一作者: 硕士研究生(何洪源教授和栾玉静研究员为共同通信作者,E-mail:13311296819@189.cn;18836705@qq.com)

收稿日期: 2024-08-09

  修回日期: 2024-10-12

  网络出版日期: 2025-08-01

基金资助

中国人民公安大学刑事科学技术双一流创新研究专项(2023SYL06)

Research on rapid screening of 90 kinds of illegally added phosphodiesterase type 5 inhibitors in liquid health foods using surface-enhanced Raman spectroscopy

  • LI Yujing ,
  • HE Hongyuan ,
  • LUAN Yujing
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  • 1(School of Criminal Investigation, People’s Public Security University of China, Beijing 100038, China)
    2(Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China)

Received date: 2024-08-09

  Revised date: 2024-10-12

  Online published: 2025-08-01

摘要

近年来,一些不法商贩向抗疲劳、壮阳和调节免疫力等类别保健食品中非法添加磷酸二酯酶5型抑制剂(phosphodiesterase type 5 inhibitors, PDE5is),严重威胁消费者健康,为提高市场监管和筛查效率,亟需建立相关现场快速检测方法。该研究基于表面增强拉曼光谱技术,以自制金溶胶为增强试剂,盐酸氯化钠溶液为匹配剂,使用乙腈和硫酸溶液进行样品前处理,建立了液体保健食品中90种非法添加PDE5is的筛查方法。该方法灵敏度高、准确性好,检出限为1~10 μg/mL,为快速检测保健食品中非法添加药物提供了可靠的技术参考。

本文引用格式

李玉静 , 何洪源 , 栾玉静 . 基于表面增强拉曼光谱法快速筛查液体保健食品中90种非法添加磷酸二酯酶5型抑制剂研究[J]. 食品与发酵工业, 2025 , 51(14) : 372 -379 . DOI: 10.13995/j.cnki.11-1802/ts.040698

Abstract

In recent years, some unscrupulous vendors have illegally added phosphodiesterase type 5 inhibitors (PDE5is) to health foods such as anti-fatigue, male enhancement, and immune regulation, posing a significant threat to consumer health.To enhance market regulation and screening efficiency, it is urgent to establish relevant on-site rapid detection methods.This study established a screening method for 90 kinds of illegal PDE5is additives in liquid health foods based on surface-enhanced Raman spectroscopy technology, using homemade gold colloid as the enhancing reagent, sodium chloride hydrochloric acid solution as the aggregating agent, and acetonitrile and sulfuric acid solution for sample pretreatment.This method boasts high sensitivity and accuracy, with detection limits ranging from 1 to 10 μg/mL, providing a technical reference for the rapid detection of illicit drugs in health supplements.

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