食品与发酵工业 >

2025 , Vol. 51 >Issue 13: 92 - 100

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040771

研究报告

青稞酒糟活性提取物对酒精性肝损伤小鼠的保护作用

  • 冯晓焕 ,
  • 罗毅皓 ,
  • 冯声宝 ,
  • 孙万成 ,
  • 李善文
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  • 1(青海大学 农牧学院,青海 西宁,810016)
    2(青海互助天佑德青稞酒股份有限公司,青海 海东,810700)
第一作者: 硕士研究生(罗毅皓副教授为通信作者,E-mail:291649347@qq.com)

收稿日期: 2024-08-16

  修回日期: 2024-09-11

  网络出版日期: 2025-08-04

收起

Protective effect of active extract of highland barley distiller’s grains on alcoholic liver injury in mice

  • FENG Xiaohuan ,
  • LUO Yihao ,
  • FENG Shengbao ,
  • SUN Wancheng ,
  • LI Shanwen
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  • 1(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)
    2(Qinghai Huzhu Tianyoude Barley Wine Co.Ltd., Haidong 810700, China)

Received date: 2024-08-16

  Revised date: 2024-09-11

  Online published: 2025-08-04

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摘要

为研究不同的青稞酒糟(highland barley distiller’s grains,HBDG)活性提取物对酒精性肝损伤(alcoholic liver injury,ALI)小鼠的影响,该文以HBDG粉为原料提取HBDG多肽和HBDG多酚,通过连续灌胃52°天佑德白酒14 d,诱导C57BL/6 J小鼠建立ALI小鼠模型。观察并记录小鼠的翻正消失时长和恢复时长;苏木精伊红染色观察小鼠肝组织形态病理变化;采用试剂盒检测分析小鼠血清、肝脏生化指标;基于非靶代谢组学检测肝脏代谢物变化。结果表明,HBDG多肽和HBDG多酚干预显著减少小鼠翻正恢复时长(P<0.05),改善由于长期饮酒导致的炎性细胞浸润和脂肪空泡化现象。与对照组相比,HBDG多肽实验组的血清谷草转氨酶(aspartate aminotransferase,AST)和甘油三酯(triglyceride,TG)水平显著降低(30.83%,44.35%)(P<0.05),肝脏乙醛脱氢酶(acetaldehyde dehydrogenase,ALDH)和超氧化物歧化酶(superoxide dismutase,SOD)水平显著升高(56.23%,49.96%)(P<0.05);此时,HBDG多酚实验组AST和TG水平显著降低(29.08%,33.87%)(P<0.05),肝脏ALDH和SOD水平升高(12.80%,11.21%)。且相比较HBDG多酚组,HBDG多肽处理下ALDH和SOD水平显著升高(38.73%,34.87%),所以HBDG多肽对机体的解酒作用和抗氧化作用高于HBDG多酚,具有显著差异(P<0.05)。肝脏代谢组学分析显示,HBDG多肽可能通过干预花生四烯酸代谢途径上调前列腺素F2α等肝脏代谢物含量改善小鼠酒精肝损伤;HBDG多酚处理可能通过影响亚油酸代谢途径上调13-OxoODE和8(R)-Hydroperoxylinoleic acid以及影响硫胺素代谢上调硫胺素含量,缓解小鼠酒精肝损伤。综上所述,HBDG多肽和HBDG多酚通过加速酒精代谢、提高抗氧化能力和调节肝脏代谢途径有效改善ALI,且HBDG多肽效果优于HBDG多酚。研究结果可为开发新的解酒护肝产品提供理论基础。

关键词： 青稞酒糟; 多肽; 多酚; 酒精性肝损伤; 保肝作用

本文引用格式

冯晓焕 , 罗毅皓 , 冯声宝 , 孙万成 , 李善文 . 青稞酒糟活性提取物对酒精性肝损伤小鼠的保护作用[J]. 食品与发酵工业, 2025 , 51(13) : 92 -100 . DOI: 10.13995/j.cnki.11-1802/ts.040771

Abstract

To investigate the effects of different highland barley distiller’s grains (HBDG) active extracts on mice with alcoholic liver injury (ALI), HBDG polypeptides and HBDG polyphenols were extracted from HBDG powder, and ALI C57BL/6 J mice model was established through continuous astringent administration of 52° Tianyoude liquor for 14 days.The time of disappearance and recovery of intoxicated mice were observed and recorded;the morphopathological changes of liver were monitored by HE staining;serum and liver biochemical indices were measured by assay kit;and changes in liver metabolites were detected based on non-target metabolomics.Results showed that HBDG peptide and HBDG polyphenols intervention significantly reduced wake-up time (P<0.05) and improved inflammatory cell infiltration and lipid vacuolization caused by prolonged drinking.Compared with the control group, serum aspartate aminotransferase (AST) and triglyceride (TG) were significantly reduced (30.83%,44.35%) (P<0.05), while hepatic acetaldehyde dehydrogenase (ALDH) and superoxide dismutase (SOD) were remarkably increased (56.23%,49.96%) (P<0.05) in the HBDG peptide group.At this time, AST and TG of HBDG polyphenol experimental group were remarkably decreased (29.08%,33.87%) (P<0.05), and hepatic ALDH and SOD increased (12.80%,11.21%).In addition, ALDH and SOD levels were significantly improved (29.08%,33.87%) (P<0.05) in the experimental group compared with the HBDG polyphenol.Subsequently, the capacity of HBDG peptides to degrade alcohol and act as antioxidants surpassed that of HBDG polyphenols, showing a notable disparity (P<0.05).Analysis of liver metabolomics revealed that HBDG polypeptides could improve alcohol-induced liver damage by interfering with the arachidonic acid metabolic pathway to up-regulate the content of hepatic metabolites like prostaglandin F2α.Additionally, HBDG polyphenol might ameliorate alcohol-related liver by interfering with the linoleic acid metabolic to up-regulate 13-OxoODE and 8(R)-Hydroperoxylinoleic acid and impact thiamine metabolism to increase thiamine content, thereby alleviating alcohol-related liver harm.In summary, the use of HBDG peptides and HBDG polyphenols proved beneficial in enhancing ALI through the hastening of alcohol metabolism, bolstering antioxidant abilities, and controlling liver metabolic routes, with HBDG peptides outperforming HBDG polyphenols.The results can provide a theoretical basis for the study of new anti-alcohol and liver protection products.

Key words： highland barley distiller’s grains; polypeptide; polyphenol; alcoholic liver injury; hepatoprotective effect

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