食品与发酵工业 >

2025 , Vol. 51 >Issue 15: 136 - 143

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040986

研究报告

指纹图谱结合化学模式识别及抗氧化活性分析冬虫夏草发酵菌丝质量

  • 陈万轩 ,
  • 张豪申 ,
  • 张雯 ,
  • 王娜 ,
  • 张剑霜
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  • (贵州师范大学 生命科学学院,贵州 贵阳,550025)
第一作者:硕士研究生(张剑霜副教授为通信作者,E-mail:assassin1986@126.com)

收稿日期: 2024-09-08

  修回日期: 2024-11-25

  网络出版日期: 2025-08-22

基金资助

:贵州省自然科学项目(黔科合基础ZK[2022]一般323);国家自然科学基金项目(82060779);贵州师范大学学术新苗基金项目(黔师新苗[2021]A31号)

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Quality evaluation of fermented Ophiocordyceps sinensis mycelia using fingerprint analysis combined with chemometric methods and antioxidant activity

  • CHEN Wanxuan ,
  • ZHANG Haoshen ,
  • ZHANG Wen ,
  • WANG Na ,
  • ZHANG Jianshuang
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  • (School of Life Sciences, Guizhou Normal University, Guiyang 550025, China)

Received date: 2024-09-08

  Revised date: 2024-11-25

  Online published: 2025-08-22

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摘要

基于指纹图谱结合化学模式识别法、多成分含量测定方法,探究基于不同菌株的冬虫夏草发酵菌丝品质差异。采用HPLC法建立24批冬虫夏草发酵菌丝指纹图谱,利用相似度评价、分层聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)、正交偏最小二乘法判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)和层次分析(analytic hierarchy process,AHP)-熵权法(entropy weight method,EWM)对其进行综合质量评估。24批冬虫夏草发酵菌丝指纹图谱相似度为0.921~0.984,标定15个共有峰,指认出5种成分;HCA和PCA均能实现不同来源冬虫夏草发酵菌丝的区分鉴别,OPLS-DA筛选出影响冬虫夏草发酵菌丝质量的5种差异标志物;冬虫夏草菌丝中腺苷、鸟苷、尿苷和胞苷含量分别为0.802~2.701、0.466~2.516、0.458~1.740、0.094~0.233 mg/g;抗氧化结果表明,四川理塘和四川黑水的冬虫夏草具有更强的DPPH自由基、ABTS阳离子自由基清除能力和还原力;AHP-EWM评价显示,四川理塘、四川黑水和西藏那曲的冬虫夏草综合评分更高。该研究建立的指纹图谱方便可靠,可有效评价不同来源冬虫夏草发酵菌丝质量。

关键词： 冬虫夏草菌; 核苷类; 抗氧化活性; 指纹图谱; 高效液相色谱法

本文引用格式

陈万轩 , 张豪申 , 张雯 , 王娜 , 张剑霜 . 指纹图谱结合化学模式识别及抗氧化活性分析冬虫夏草发酵菌丝质量[J]. 食品与发酵工业, 2025 , 51(15) : 136 -143 . DOI: 10.13995/j.cnki.11-1802/ts.040986

Abstract

This study investigates the quality differences of fermented Ophiocordyceps sinensis mycelia from various strains by utilizing fingerprint analysis, chemometric methods, and multi-component content determination.High-performance liquid chromatography (HPLC) was used to establish fingerprints for 24 batches of O.sinensis fermented mycelia.Then, the quality of the mycelia was evaluated through similarity assessment, hierarchical cluster analysis(HCA), principal component analysis(PCA), orthogonal partial least squares-discriminant analysis(OPLS-DA), analytic hierarchy process(AHP) and entropy weight method(EWM).Fingerprint similarity across batches, originating from 8 strains, ranged from 0.921 to 0.984.Fifteen common peaks were identified, and five compounds were characterized from fingerprints.Both HCA and PCA can effectively differentiate the O.sinensis mycelia from varying sources, and 5 compounds responsible for quality variation were screened out by OPLS-DA.The contents of adenosine, guanosine, uridine, and cytidine were found to range from 0.802 to 2.701 mg/g, 0.466 to 2.516 mg/g, 0.458 to 1.740 mg/g, and 0.094 to 0.233 mg/g, respectively.Antioxidant activity assays indicated that the samples of LT and HS exhibited superior DPPH free radical and ABTS cationic radical scavenging activities, and reducing power.AHP-Entropy Weight analysis showed that LT, HS and NQ achieved higher overall scores than those of others.The established fingerprint analysis method is reliable and convenient, which provides an effective approach for evaluating the quality of O.sinensis fermented products.

Key words： Ophiocordyceps sinensis; nucleosides; antioxidant activities; fingerprint; high performance liquid chromatography

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