食品与发酵工业 >

2025 , Vol. 51 >Issue 15: 185 - 192

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041110

研究报告

基于分子对接技术的牡蛎酶解产物中免疫调节肽筛选及其抗氧化作用

  • 吴虹颖 ,
  • 黄倩倩 ,
  • 秦小明 ,
  • 曹文红 ,
  • 林海生
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  • (广东海洋大学 食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,广东省水产预制食品加工与品质 控制工程技术研究中心,广东 湛江,524088)
第一作者:硕士研究生(秦小明教授为通信作者,E-mail:xiaoming0502@21cn.com)

收稿日期: 2024-09-21

  修回日期: 2024-11-06

  网络出版日期: 2025-08-22

基金资助

现代农业产业技术体系专项资金(CARS-49)

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Screening of immunomodulatory peptides from oyster enzymatic hydrolysates via molecular docking technology and evaluation of antioxidant properties

  • WU Hongying ,
  • HUANG Qianqian ,
  • QIN Xiaoming ,
  • CAO Wenhong ,
  • LIN Haisheng
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  • (College of Food Science and Technology, Guangdong Ocean University, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China)

Received date: 2024-09-21

  Revised date: 2024-11-06

  Online published: 2025-08-22

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摘要

该研究基于分子对接技术筛选牡蛎提取物中的免疫活性肽,并通过体外实验探究其免疫调节和抗氧化作用。在分子对接结果中,LLQLGSGR(OP1)和DNSIAMESMK(OP2)肽段与Toll样受体2和Toll样受体4的结合能分别为-7.7、-6.1 kcal/mol和-6.3、-4.1 kcal/mol,展现出较强的结合能力。在体外细胞实验中,OP1在200 μg/mL下显著增加了RAW264.7细胞NO的产量(P<0.05),并在100 μg/mL下促进了细胞增殖、炎症因子的分泌以及细胞吞噬功能,吞噬指数达到1.45±0.04(P<0.001)。OP2在100 μg/mL下,显著增强了RAW264.7细胞TNF-α的分泌(P<0.01),也表现出良好的免疫调节作用。在抗氧化实验中,OP1与OP2在200 μg/mL下的DPPH自由基清除率分别达到49.10%、56.32%,羟自由基清除率分别达到37.82%、41.46%,同时在超氧阴离子清除实验中均表现出较好的清除效果,显示出较强的抗氧化能力。综上,OP1和OP2展示了良好的免疫调节作用和抗氧化活性,为开发天然免疫调节剂和功能性食品提供了理论依据。

关键词： 牡蛎; 分子对接; RAW264.7细胞; 免疫调节; 抗氧化活性

本文引用格式

吴虹颖 , 黄倩倩 , 秦小明 , 曹文红 , 林海生 . 基于分子对接技术的牡蛎酶解产物中免疫调节肽筛选及其抗氧化作用[J]. 食品与发酵工业, 2025 , 51(15) : 185 -192 . DOI: 10.13995/j.cnki.11-1802/ts.041110

Abstract

Screening of immunoactive peptides from oyster extracts was conducted using molecular docking technology, followed by in vitro experiments to assess their immunomodulatory and antioxidant properties.The results of molecular docking indicated that peptides LLQLGSGR (OP1) and DNSIAMESMK (OP2) exhibited strong binding affinities to Toll-like receptor 2 and Toll-like receptor 4, with binding energies of -7.7, -6.1 kcal/mol, and -6.3, -4.1 kcal/mol, respectively.The experiments in vitro with RAW264.7 cells showed that OP1, at a concentration of 200 μg/mL, significantly increased NO production (P<0.05) and enhanced cell proliferation, inflammatory cytokine secretion, and phagocytic activity at a concentration of 100 μg/mL, achieving a phagocytic index of 1.45±0.04 (P<0.001).OP2, at a concentration of 100 μg/mL, notably boosted TNF-α secretion (P<0.01), further demonstrating its immunomodulatory effects.In antioxidant assays, OP1 and OP2 exhibited DPPH radical scavenging rates of 49.10% and 56.32%, respectively, at 200 μg/mL, alongside hydroxyl radical scavenging rates of 37.82% and 41.46%.Both peptides also displayed significant superoxide anion scavenging activity, underscoring their robust antioxidant properties.In conclusion, OP1 and OP2 demonstrated substantial immunomodulatory and antioxidant activities, offering a promising foundation for the development of natural immunomodulators and functional foods.

Key words： oysters; molecular docking; RAW264.7 cells; immunoregulation; antioxidant activity

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