食品与发酵工业 >

2025 , Vol. 51 >Issue 16: 134 - 141

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042820

研究报告

虾头活性多肽对力竭游泳小鼠氧化应激所致疲劳的缓解作用研究

  • 彭广飞 ,
  • 张世奇 ,
  • 万永伦 ,
  • 吕小洪 ,
  • 谢宝灵 ,
  • 周针先 ,
  • 熊欢 ,
  • 邓同兴
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  • 1(漯河医学高等专科学校,河南 漯河,462000)
    2(岭南师范学院食品科学与工程学院,广东 湛江,524048)
    3(重庆第二师范学院 生物与化学工程学院,重庆,400067)
第一作者:学士,讲师(邓同兴副教授为通信作者,E-mail:345782607@qq.com)

收稿日期: 2025-03-25

  修回日期: 2025-04-08

  网络出版日期: 2025-08-29

基金资助

河南省科技厅科技攻关项目(242102310088);广东省基础与应用基础研究基金自然科学基金项目(2022A1515010360);漯河医学高等专科学校校本部2024年度科技创新项目(KJCX202416)

收起

Mitigating effect of active peptides from shrimp heads on fatigue induced by oxidative stress in exhausted swimming mice

  • PENG Guangfei ,
  • ZHANG Shiqi ,
  • WAN Yonglun ,
  • LYU Xiaohong ,
  • XIE Baoling ,
  • ZHOU Zhenxian ,
  • XIONG Huan ,
  • DENG Tongxing
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  • 1(Luohe Medical College, Luohe 462000, China)
    2(College of Laboratory of Food Science and Engineering, Lingnan Normal University, Zhanjiang 524048, China)
    3(School of Biological&Chemical Engineering, Chongqing University of education, Chongqing 400067, China)

Received date: 2025-03-25

  Revised date: 2025-04-08

  Online published: 2025-08-29

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摘要

为研究虾头活性多肽对力竭游泳小鼠氧化应激所致疲劳的缓解作用。首先,通过测定SP(虾头活性多肽)的DPPH自由基和羟自由基清除能力,评价其抗氧化性能。然后,连续28 d对各组小鼠进行低、中、高剂量的SP灌胃处理,测定其负重游泳时间、抗氧化相关生化指标以及核因子E2相关因子2 (nuclear factor erythtoid 2 related factor 2, Nrf2)、血红素氧化酶1(heme oxygenase 1, HO-1)和细胞凋亡标志蛋白(cleaved caspase 3, C-caspase 3)的mRNA表达水平。结果表明,在质量浓度为6.0~8.0 mg/mL时,SP对DPPH自由基清除率(83.04%)和羟自由基清除率(94.50%)效果最优;SP各剂量组小鼠负重游泳力竭时间较正常空白组组均显著延长(P<0.05),SP中、高剂量组过氧化氢酶、总超氧化物歧化酶、钠钾三磷酸腺嘌呤核苷酸酶和钙镁三磷酸腺嘌呤核苷酸酶的活性显著增强(P<0.05),Nrf2和HO-1 mRNA表达显著上调,C-caspase 3 mRNA表达显著下调(P<0.05)。综上所述,体外自由基清除实验与动物实验结果表明SP抗氧化效果显著,能够减轻小鼠的氧化应激症状,显著缓解小鼠的疲劳,暗示其具有成为新型功能性食品或抗疲劳药物的潜力。

关键词： 虾头活性多肽; 抗氧化作用; Nrf2/HO-1信号通路; 力竭游泳小鼠; 凡纳滨对虾

本文引用格式

彭广飞 , 张世奇 , 万永伦 , 吕小洪 , 谢宝灵 , 周针先 , 熊欢 , 邓同兴 . 虾头活性多肽对力竭游泳小鼠氧化应激所致疲劳的缓解作用研究[J]. 食品与发酵工业, 2025 , 51(16) : 134 -141 . DOI: 10.13995/j.cnki.11-1802/ts.042820

Abstract

To investigate the antioxidant stress and anti-fatigue effects of shrimp head active polypeptide (SP) on mice with exhaustive swimming, the DPPH free radical scavenging ability and hydroxyl radical (·OH) scavenging ability of SP were first measured to evaluate its antioxidant performance.Then, mice in each group were gavaged with low, medium, and high doses of SP for 28 consecutive days.Mice exhaustive swimming time, antioxidant related biochemical indices, as well as the mRNA expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), and cleaved caspase 3 (C-caspase 3) were determined. Results showed that when the mass concentration was 6.0-8.0 mg/mL, the DPPH free radical scavenging rate (83.04%) and ·OH scavenging rate (94.50%) of SP showed optimal scavenging activity.In the animal experiment, the exhaustive swimming time of mice in each dose group of SP was significantly longer than that of the normal blank group (P<0.05).The activities of catalase, total superoxide dismutase, calcium-magnesium adenosine triphosphatase, and sodium-potassium adenosine triphosphatase in the medium and high dose groups of SP were significantly enhanced (P<0.05), and the mRNA expression levels of Nrf2 and HO-1 were significantly upregulated, while the mRNA expression level of C-caspase 3 was significantly downregulated (P<0.05).In conclusion, the results from both in vitro free radical scavenging experiments and animal experiments demonstrated that SP exhibited significant antioxidant effects.It effectively alleviated oxidative stress symptoms in mice while markedly reducing fatigue manifestations.These findings suggested its potential as a novel functional food or anti-fatigue therapeutic agent.

Key words： shrimp head autolytic active peptides; antioxidant effect; Nrf2/HO-1 signaling pathway; exhausted swimming mice; Litopenaeus vannamei

参考文献

[1] 王思炀. 我国去年对虾产量增长6.4%, 广东位居第一[EB/OL].[2024-06-21]2024-0621.https://www.163.com/dy/article/J577CBGD055284JB.html.
[2] HOSNEY A, ULLAH S, BARČAUSKAIT K.A review of the chemical extraction of chitosan from shrimp wastes and prediction of factors affecting chitosan yield by using an artificial neural network[J].Marine Drugs, 2022, 20(11):675.
[3] MA Y Y, ZHANG D D, LIU M Q, et al. Identification of antioxidant peptides derived from Tilapia (Oreochromis niloticus) skin and their mechanism of action by molecular docking[J]. Foods, 2022, 11(17):2576.
[4] LYU R Z, DONG Y F, BAO Z J, et al. Advances in the activity evaluation and cellular regulation pathways of food-derived antioxidant peptides[J]. Trends in Food Science & Technology, 2022, 122:171-186.
[5] PAN X X, XU J, JIA X M. Research progress evaluating the function and mechanism of anti-tumor peptides[J]. Cancer Management and Research, 2020, 12:397-409.
[6] LEIVA-PORTILLA D, MARTÍNEZ R, BERNAL C. Valorization of shrimp (Heterocarpus reedi) processing waste via enzymatic hydrolysis: Protein extractions, hydrolysates and antioxidant peptide fractions[J]. Biocatalysis and Agricultural Biotechnology, 2023, 48:102625.
[7] MECHRI S, JABALLI I, BEN TAHEUR F, et al.Anti-biofilm, antioxidant and cytotoxic potential of f5, a peptide derived from waste generated during the processing of the white shrimp, Metapenaeus monoceros (Fabricius, 1798)[J].Waste and Biomass Valorization, 2022, 13(7):3233-3244.
[8] WANG P X, ZENG H L, LIN S L, et al.Anti-fatigue activities of hairtail (Trichiurus lepturus) hydrolysate in an endurance swimming mice model-ScienceDirect[J].Journal of Functional Foods, 2020, 74:104207.
[9] 彭元怀, 王睿, 王凤妮, 等.基于Keap1/Nrf2/ARE信号通路研究虾头自溶活性多肽对小鼠抗疲劳的作用及其机制[J].食品工业科技, 46(5):364-370.
PENG Y H, WANG R, WANG F N, et al.Investigation the Effects and Mechanisms of Autolytic Active Peptides from Shrimp Head on Anti-fatigue in Mice Based on the Keap1/Nrf2/ARE Signaling Pathway[J].Science and Technology of Food Industry, 2025, 46(5):364-370.
[10] 姜丽冬, 金鑫, 朴春红, 等.林蛙油生物活性肽的制备工艺及其抗疲劳研究[J].吉林农业大学学报, 2016, 38(4):482-488.
JIANG L D, JIN X, PIAO C H, et al.Preparation technology and antifatigue effects of oviductus ranae bioactive peptide[J].Journal of Jilin Agricultural University, 2016, 38(4):482-488.
[11] 李欣. 鹿茸菇多糖对小鼠抗疲劳能力的影响[J].食品研究与开发, 2022, 43(16):44-50.
LI X.Anti-fatiuge effects of Lyophyllum decastes polysaccharides in mice[J].Food Research and Development, 2022, 43(16):44-50.
[12] 刘菲霞, 李红娟, 徐强, 等.滑菇子实体多糖提取条件优化及抗氧化活性研究[J].山东农业科学, 2013, 45(1):117-121.
LIU F X, LI H J, XU Q, et al.Condition optimization for extracting pholiota nameko fruiting body polysaccharide and study on its antioxidant activity[J].Shandong Agricultural Sciences, 2013, 45(1):117-121.
[13] 张世奇, 王睿, 李成良, 等.辣木叶多糖对STZ诱导糖尿病小鼠的降糖效果及其机制[J].食品工业科技, 2024, 45(19):357-365.
ZHANG S Q, WANG R, LI C L, et al.hypoglycemic effect of moringa oleifera leaf polysaccharide on STZ-induced diabetic mice and its mechanism[J].Science and Technology of Food Industry, 2024, 45(19):357-365.
[14] ZHOU Y F, XU Y X, TIAN T G, et al. Antihypertensive and antioxidant effects of food-derived bioactive peptides in spontaneously hypertensive rats[J]. Food Science & Nutrition, 2024, 12(10):8200-8210.
[15] 张皓哲, 高春红, 李天铎, 等. 胶原和明胶清除羟基自由基机理探究[J]. 齐鲁工业大学学报, 2022, 36(3):32-38.
ZHANG H Z, GAO C H, LI T D, et al. Study on the mechanism of collagen and gelatin scavenging hydroxyl radical[J]. Journal of Qilu University of Technology, 2022, 36(3):32-38.
[16] XU Z L, HU Q H, XIE M H, et al. Protective effects of peptide KSPLY derived from Hericium erinaceus on H2O2-induced oxidative damage in HepG2 cells[J]. Food Science and Human Wellness, 2023, 12(5):1893-1904.
[17] WANG W Q, LIU Z C, LIU Y J, et al.Plant polypeptides:A review on extraction, isolation, bioactivities and prospects[J].International Journal of Biological Macromolecules, 2022, 207:169-178.
[18] WANG X Q, YU H A, XING R E, et al.Hepatoprotective effect of oyster peptide on alcohol-induced liver disease in mice[J].International Journal of Molecular Sciences, 2022, 23(15):8081.
[19] WANG X L, JIANG R W.Therapeutic potential of superoxide dismutase fused with cell-penetrating peptides in oxidative stress-related diseases[J].Mini Reviews in Medicinal Chemistry, 2022, 22(17):2287-2298.
[20] 习雪峰, 王单一, 熊正英, 等.云芝多糖对运动训练大鼠脑组织抗氧化能力和ATPase活性的影响[J].食品科学, 2012, 33(5):256-259.
XI X F, WANG D Y, XIONG Z Y, et al.Effect of krestin polysaccharide on antioxidant capability and atpase activity in brain tissues of rats with high intensity exercise[J].Food science, 2012, 33(5):256-259.
[21] DENT J R, STOCKS B, CAMPELJ D G, et al.Transient changes to metabolic homeostasis initiate mitochondrial adaptation to endurance exercise[J].Seminars in Cell & Developmental Biology, 2023, 143:3-16.
[22] SUN Y, SHEN H R, FAN C Z, et al.Typical structural characteristics and hepatoprotective effects of novel high fischer ratio oligopeptides from antarctic krill on acute alcoholic liver injury[J].Food & Function, 2024, 15(18):9298-9314.
[23] KIM C, KIM N, BAE J S.Anti-Inflammatory and Anti-oxidant functions of cornuside by regulating Nf-κb, Stat-1, and Nrf2-HO-1 pathways[J].The American Journal of Chinese Medicine, 2022, 50(5):1349-1360.
[24] ZHANG C Y, HU X C, ZHANG G Z, et al.Role of Nrf2 and HO-1 in intervertebral disc degeneration[J].Connective Tissue Research, 2022, 63(6):559-576.
[25] 张睿玉. 灰树花多肽的制备及其抗氧化活性研究[D].晋中:山西农业大学, 2022:55-57.
ZHANG R S.Study on the preparation and antioxidant activity of polypeptide from Grifola frondosa[D].Jinzhong:Shanxi Agricultural University, 2022:55-57.
[26] 黄晓巍, 刘玥欣, 王晋冀, 等.鹿茸多肽对轻度认知功能障碍大鼠学习记忆能力的改善作用及其调节Keap1/Nrf2/HO-1信号通路的机制[J].吉林大学学报(医学版), 2021, 47(5):1215-1220.
HUANG X W, LIU Y X, WANG J J, et al.Improvement effects of pilose antler polypeptide on learning and memory abilities in rats with mild cognitive impairment and their mechanisms of regulating Keap1/Nrf2 /HO-1 signaling pathway[J].Journal of Jilin University (Medicine Edition), 2021, 47(5):1215-1220.
[27] CHANDIMALI N, BAK S G, PARK E H, et al.Free radicals and their impact on health and antioxidant defenses:A review[J].Cell Death Discovery, 2025,11(1): 19.
[28] 杨福愉. 生物膜结构研究的一些进展[J].生物化学与生物物理进展, 2003, 30(4):495-502.
YANG F Y. Recent advances in the study of membrane structure[J]. Progress in Biochemistry and Biophysics, 2003, 30(4):495-502.
[29] 徐雷, 杨磊, 杜柳涛, 等. 静态负荷对大鼠离体骨骼肌能量代谢的影响[J]. 中国工业医学杂志, 2005, 18(1):14-15;18.
XU L, YANG L, DU L T, et al. Effect of static load on energy metabolism of rat’s isolated skeletal muscles[J]. Chinese Journal of Industrial Medicine, 2005, 18(1):14-15; 18.
[30] 孙鑫, 杨芳, 邓洋洋, 等.补肾, 健脾, 活血方法对骨质疏松症小鼠骨及骨骼肌中Ca2+-Mg2+-ATP酶含量影响[J].中国中医基础医学杂志, 2015,21(4):416-417.
SUN X, YANG F, DENG Y Y, et al.Effect of Bushen Jianpi Huoxue Method to the Ca2+-Mg2+-ATP, enzyme content of bone and skeletal muscle in osteoporosis mice[J].Journal of Basic Chinese Medicine, 2015,21(4):416-417.
[31] ITOH K, WAKABAYASHI N, KATOH Y, et al.Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain[J].Genes & Development, 1999, 13(1):76-86.
[32] OTTERBEIN L E, BACH F H, ALAM J,et al.Carbon monoxide has anti-inflammatory effects involving the mitogen-activated protein kinase pathway[J].Nature Medicine, 2000, 6(4):422-428.
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