该研究旨在比较鲟鱼籽酶解液冻干粉(sturgeon roe enzymatic hydrolysate powder,SREHP)与市售解酒玉米低聚肽(corn oligopeptide, COP)在肝损伤保护及抗氧化能力方面的差异。构建酒精诱导的HepG2细胞氧化损伤模型,研究不同蛋白浓度(0.2、0.5、1.0 mg/mL)的SREHP及COP对肝损伤细胞的保护效果,通过测定天冬氨酸转氨酶(aspartate aminotransferase,AST)、丙氨酸转氨酶(alanine aminotransferase,ALT)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(total antioxidant capacity,T-AOC)以及细胞凋亡水平等多项生化指标来对比评估两者的抗氧化能力。结果显示,经800 mmol/L乙醇诱导损伤24 h后的细胞活力为51.29%,造模成功。在0.2、0.5、1.0 mg/mL SREHP作用下,HepG2细胞的存活率分别提高了25.73%、51.11%、75.24%,均高于同等蛋白浓度下的COP处理组。经SREHP、COP预保护的T-AOC分别提高了10.67%和6.53%。此外,与COP组相比,SREHP组在降低AST、ALT和MDA水平以及增强SOD活性方面,均表现出更显著的优势,研究结果为开发新型抗氧化剂及功能性解酒产品提供了科学依据。
This study aimed to compare the liver-protective and antioxidant capacities of sturgeon roe enzymatic hydrolysate powder (SREHP) and commercially available corn oligopeptide (COP).An alcohol-induced oxidative damage model using HepG2 cells was established to evaluate the protective effects of SREHP and COP at various protein concentrations (0.2, 0.5, 1.0 mg/mL).Key biochemical indicators, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and cell apoptosis levels, were analyzed to assess antioxidant capacity.Results showed that the cell viability of HepG2 cells after 24 h of exposure to 800 mmol/L ethanol decreased to 51.29%, confirming successful model establishment.Treatment with 0.2, 0.5, and 1.0 mg/mL SREHP increased cell survival rates by 25.73%, 51.11%, and 75.24%, respectively, surpassing the COP-treated groups at equivalent concentrations.Pre-treatment with SREHP and COP enhanced T-AOC by 10.67% and 6.53%, respectively.Furthermore, SREHP exhibited significantly greater efficacy than COP in reducing AST, ALT, and MDA levels and in enhancing SOD activity.These findings provide a scientific basis for the development of novel antioxidants and functional anti-alcohol products.
[1] MACKOWIAK B, FU Y J, MACCIONI L, et al.Alcohol-associated liver disease[J].The Journal of Clinical Investigation, 2024, 134(3):e176345.
[2] NG W J, WONG F C, ABD MANAN F, et al.Antioxidant peptides and protein hydrolysates from tilapia:Cellular and in vivo evidences for human health benefits[J].Foods, 2024, 13(18):2945.
[3] YANG X T, TANG C H, ZHAO Q Y, et al.Melanin:A promising source of functional food ingredient[J].Journal of Functional Foods, 2023, 105:105574.
[4] 中华人民共和国农业农村部. 2022年农业主推技术[M].北京:中国农业出版社, 2023.
[5] WEI Q W, ZOU Y, LI P, et al.Sturgeon aquaculture in China:Progress, strategies and prospects assessed on the basis of nation-wide surveys (2007—2009)[J].Journal of Applied Ichthyology, 2011, 27(2):162-168.
[6] LI X F, XIE W, BAI F, et al.Influence of thermal processing on flavor and sensory profile of sturgeon meat[J].Food Chemistry, 2022, 374:131689.
[7] 尹剑, 武瑞赟, 阿热爱·巴合提, 等.酶解法制备鲟鱼皮活性肽条件优化及抗氧化能力[J].肉类研究, 2020, 34(2):33-39.
YIN J, WU R Y, AREAI B, et al.Optimization of the conditions for the enzymatic preparation of antioxidant peptides from sturgeon skin[J].Meat Research, 2020, 34(2):33-39.
[8] 李杰伦. 鲟鱼头活性物质提取工艺优化及生产车间设计[D].大连:大连海洋大学, 2024.
LI J L.Optimization of extraction process of active substances insturgeon head and design of production workshop[D].Dalian:Dalian Ocean University, 2024.
[9] 饶承冬, 柯勤勤, 郑杨杨, 等.鲟鱼鱼肠抗氧化肽的提取及抗氧化性[J].基因组学与应用生物学, 2020, 39(1):88-95.
RAO C D, KE Q Q, ZHENG Y Y, et al.Preparation and antioxidant activity of antioxidant peptides from sturgeon(Acipenser sinensis) intestine[J].Genomics and Applied Biology, 2020, 39(1):88-95.
[10] XU L J, LIU Y, JIAO Y, et al.Enzyme-free method for preparation of sturgeon extracts with antioxidant, hepatoprotective and immune-enhancing functions[J].Food Chemistry, 2024, 459:140327.
[11] 张李君, 赵甜甜, 陈杰琼, 等.鲟鱼子酶解产物对酒精损伤肝细胞的保护作用及活性肽虚拟筛选[J].食品工业科技, 2024, 45(19):316-324.
ZHANG L J, ZHAO T T, CHEN J Q, et al.Protective effects of enzymatic products from sturgeon roe on alcohol-induced hepatic cell damage and virtual screening of active peptides[J].Science and Technology of Food Industry, 2024, 45(19):316-324.
[12] DE SEVILLA U, BAUTISTA J.Ergothioneine rich Agaricus bisporus extracts decreases lipid accumulation induced by oleic acid in HepG2 cells:Possible implications in the treatment of nonalcoholic liver fatty disease[J].Food Science and Nutrition, 2019, 5(2):1-7.
[13] 刘晓凤, 卢晓琴, 钟浩, 等.人参皂苷对过氧化氢诱导的Caco-2细胞氧化损伤的保护作用[J].食品与发酵工业, 2024, 50(7):46-50.
LIU X F, LU X Q, ZHONG H, et al.Protective effect of ginsenosides on hydrogen peroxide-induced oxidative damage of Caco-2 cells[J].Food and Fermentation Industries, 2024, 50(7):46-50.
[14] 杨彬君, 冯彩玲, 吴岩斌, 等.牛樟芝多糖对HepG2细胞酒精性氧化损伤的保护作用[J].菌物学报, 2021, 40(6):1549-1560.
YANG B J, FENG C L, WU Y B, et al.Protective effect of Taiwanofungus camphoratus polysaccharides on alcohol-induced oxidative injury in HepG2 cells[J].Mycosystema, 2021, 40(6):1549-1560.
[15] ZHANG M J, ZHANG G S, MENG X X, et al.Reduction of the oxidative damage to H2O2-induced HepG2 cells via the Nrf2 signalling pathway by plant flavonoids quercetin and hyperoside[J].Food Science and Human Wellness, 2024, 13(4):1864-1876.
[16] GUO Y S, CAI X S, LU H W, et al.17β-estradiol promotes apoptosis of HepG2 cells caused by oxidative stress by increasing Foxo3a phosphorylation[J].Frontiers in Pharmacology, 2021, 12:607379.
[17] HU Y D, ZHANG H, LU R Q, et al.Enabling the biosynthesis of Antroquinonol in submerged fermentation of Antrodia camphorata[J].Biochemical Engineering Journal, 2014, 91:157-162.
[18] 张丽杰. 血清转氨酶的测定及临床意义[J].中国医药指南, 2012, 10(9):298-299.
ZHANG L J.Determination and clinical significance of serum transaminase[J].Guide of China Medicine, 2012, 10(9):298-299.
[19] 廖彩玉. 松脂皱皮孔菌多糖提取、抗氧化及对酒精性肝细胞损伤的保护作用[D].长春:吉林大学,2023.
LIAO Caiyu.Extraction, antioxidant, and protective effects on alcoholic hepatocyte damage of polysaccharide from Ischnoderma resinosum[D].Changchun:Jilin University, 2023.
[20] ISLAMIANA D, PRABOWO R, PRAMANINGTYAS M D.The effect of orange water kefir on malondialdehyde (MDA) level and superoxide dismutase (SOD) inhibition rate in kidney tissue of the hyperlipidemic rat (Rattus norvegicus)[J].Atherosclerosis, 2020, 315:e264.
[21] 周泓妍, 郑怡, 王海东, 等.南、北五味子蛋白抗氧化活性和对HepG2细胞氧化应激损伤的修复作用[J].食品与发酵工业, 2024, 50(7):51-60.
ZHOU H Y, ZHENG Y, WANG H D, et al.Antioxidant activity of Schisandra sphenanthera and Schisandra chinensis protein on HepG2 cells[J].Food and Fermentation Industries, 2024, 50(7):51-60.
[22] JIA R, DU J L, CAO L P, et al.Effects of dietary baicalin supplementation on growth performance, antioxidative status and protection against oxidative stress-induced liver injury in GIFT tilapia (Oreochromis niloticus)[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2021, 240:108914.
