研究报告

大曲清香型白酒固态发酵规模缩小体系的构建

  • 辛姝涵 ,
  • 靳光远 ,
  • 徐岩
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  • (江南大学 生物工程学院,酿造微生物与应用酶学研究室,江苏 无锡,214122)
第一作者:硕士研究生(徐岩教授为通信作者,Email:yxu@jiangnan.edu.cn)

收稿日期: 2024-11-19

  修回日期: 2024-12-26

  网络出版日期: 2025-09-29

基金资助

国家自然科学基金项目(22108101);国家重点研发计划项目(2022YFD2101202)

Scale-down of Qingxiangxing Baijiu solid-state fermentation

  • XIN Shuhan ,
  • JIN Guangyuan ,
  • XU Yan
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  • (Laboratory of Brewing Microbiology and Applied Enzymology, School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2024-11-19

  Revised date: 2024-12-26

  Online published: 2025-09-29

摘要

规模缩小(scale-down)技术作为一种强有力的工具,能够在实验室环境中模拟和优化大规模生产过程。鉴于白酒固态发酵体系的复杂性,直接对其分析颇具挑战,因此,实验室规模研究显得尤为重要。该研究聚焦于发酵体系内热量传递,以径高比作为缩放参数,确定初始发酵参数。通过对比实验室与生产规模发酵中的理化参数、微生物菌群和风味物质,评估scale-down体系与大曲清香型白酒体系之间的相似性。结果表明,2种体系在理化指标变化趋势、优势微生物种群和高丰度风味物质上均保持一致;经双单侧t检测分析,淀粉、还原糖、乳酸和乙醇的变化速率参数显示出统计学等效性,单侧检验P值均小于0.05。这些发现初步验证了scale-down模型对清香型白酒体系的适用性和以径高比作为缩放参数的scale-down体系的有效性,为白酒固态发酵工程学理论的建立奠定了基础。

本文引用格式

辛姝涵 , 靳光远 , 徐岩 . 大曲清香型白酒固态发酵规模缩小体系的构建[J]. 食品与发酵工业, 2025 , 51(17) : 135 -142 . DOI: 10.13995/j.cnki.11-1802/ts.041629

Abstract

Scale-down technology is a powerful tool for simulating and optimizing large-scale production processes in a laboratory environment.The solid-state fermentation system in Baijiu production is complex, making direct analysis difficult and emphasizing the importance of laboratory-scale studies.This study focused on heat transfer in the fermentation system, using the diameter-to-height ratio as a scaling parameter to determine the initial fermentation conditions.By comparing the physicochemical parameters, microbial populations, and flavor compounds in laboratory and production scale fermentations, we evaluated the similarities between the scale-down system and the Qingxiangxing Baijiu system.The results indicated that both systems exhibited similar trends in changes in physicochemical indicators, dominant microbial populations, and high-abundance flavor compounds.A Two-One-Sided-T test (TOST) revealed that the change rate parameters for starch, reducing sugars, lactic acid, and ethanol were statistically equivalent, with one-sided P-values <0.05.These results preliminarily confirmed the applicability of the scale-down model to the Qingxiangxing Baijiu system and validated the use of the diameter-to-height ratio as a scaling parameter, providing a foundation for developing solid-state fermentation engineering theory in Baijiu production.

参考文献

[1] TÄUBER S, GRÜNBERGER A.Microfluidic single-cell scale-down systems:Introduction, application, and future challenges[J].Current Opinion in Biotechnology, 2023, 81:102915.
[2] SARTAS M, SCHUT M, PROIETTI C, et al.Scaling Readiness:Science and practice of an approach to enhance impact of research for development[J].Agricultural Systems, 2020, 183:102874.
[3] DELVIGNE F, TAKORS R, MUDDE R, et al.Bioprocess scale-up/down as integrative enabling technology:From fluid mechanics to systems biology and beyond[J].Microbial Biotechnology, 2017, 10(5):1267-1274.
[4] TAJSOLEIMAN T, MEARS L, KRÜHNE U, et al.An industrial perspective on scale-down challenges using miniaturized bioreactors[J].Trends in Biotechnology, 2019, 37(7):697-706.
[5] FARRELL P, SUN J, GAO M, et al.Development of a scaled-down aerobic fermentation model for scale-up in recombinant protein vaccine manufacturing[J].Vaccine, 2012, 30(38):5695-5698.
[6] HARINGA C, DESHMUKH A T, MUDDE R F, et al.Euler-Lagrange analysis towards representative down-scaling of a 22 m3 aerobic S.cerevisiae fermentation[J].Chemical Engineering Science, 2017, 170:653-669.
[7] HUANG R J, WANG K, FLAMM M H, et al.Development and qualification of 3 L scale-down model for large scale vaccine process on Vero cell culture using microcarriers[J].Biotechnology and Bioengineering, 2024, 121(11):3402-3414.
[8] JIN G Y, ZHU Y, XU Y.Mystery behind Chinese liquor fermentation[J].Trends in Food Science & Technology, 2017, 63:18-28.
[9] ZHUANSUN W W, XU J, LI Z Q, et al.Dynamic changes in the microbial community, flavour components in Jiupei of a novel Maotai-Luzhou-flavoured liquor under various daqu blending modes and their correlation analysis[J].LWT, 2022, 172:114167.
[10] 赵玉杰, 靳光远, 唐群勇, 等.基于热量平衡模型的大曲发酵升温机理研究[J].食品与发酵工业, 2024, 50(14):18-25.
ZHAO Y J, JIN G Y, TANG Q Y, et al.The mechanism of heat transfer during Daqu fermentation based on heat balance modeling[J].Food and Fermentation Industries, 2024, 50(14):18-25.
[11] JIN G Y, UHL P, ZHU Y, et al.Modeling of industrial-scale anaerobic solid-state fermentation for Chinese liquor production[J].Chemical Engineering Journal, 2020, 394:124942.
[12] JIN G Y, ZHAO Y J, XIN S H, et al.Solid-state fermentation engineering of traditional Chinese fermented food[J].Foods, 2024, 13(18):3003.
[13] PETIOT C, DE GUARDIA A.Composting in a laboratory reactor:A review[J].Compost Science & Utilization, 2004, 12(1):69-79.
[14] WANG Y J, PANG L, LIU X Y, et al.Using thermal balance model to determine optimal reactor volume and insulation material needed in a laboratory-scale composting reactor[J].Bioresource Technology, 2016, 206:164-172.
[15] TIKHOMIROVA T S, TARASKEVICH M S, PONOMARENKO O V.The role of laboratory-scale bioreactors at the semi-continuous and continuous microbiological and biotechnological processes[J].Applied Microbiology and Biotechnology, 2018, 102(17):7293-7308.
[16] WANG G, XU X C, ZHOU L, et al.A pilot-scale study on the start-up of partial nitrification-anammox process for anaerobic sludge digester liquor treatment[J].Bioresource Technology, 2017, 241:181-189.
[17] WANG G, WU B F, ZHAO J F, et al.Power input effects on degeneration in prolonged penicillin chemostat cultures:A systems analysis at flux, residual glucose, metabolite, and transcript levels[J].Biotechnology and Bioengineering, 2018, 115(1):114-125.
[18] ENGINEERTOOLBOX.https://www.engineeringtoolbox.com/[Z].2001
[19] JIANG Z M, CHEN Y, WANG D L, et al.The influence of porosity of the grain medium on the flavour formation of Chinese liquor during solid-state fermentation[J].Journal of the Institute of Brewing, 2016, 122(3):468-472.
[20] LIU S Q, SKINNER-NEMEC K A, LEATHERS T D.Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of xylose and glucose into ethanol and other products[J].Journal of Industrial Microbiology & Biotechnology, 2008, 35(2):75-81.
[21] HAO M J, WU D, XU Y, et al.A novel endo-polygalacturonase from Penicillium rolfsii with prebiotics production potential:Cloning, characterization and application[J].Foods, 2022, 11(21):3469.
[22] WANG S L, XIONG W, WANG Y Q, et al.Temperature-induced annual variation in microbial community changes and resulting metabolome shifts in a controlled fermentation system[J].mSystems, 2020, 5(4):e00555-20.
[23] WU Q, ZHU Y, FANG C, et al.Can we control microbiota in spontaneous food fermentation? —Chinese liquor as a case example[J].Trends in Food Science & Technology, 2021, 110:321-331.
[24] VAURIS A, VALCAUDA S, HUSSON F, et al.A novel method to assess heat transfer and impact of relevant physicochemical parameters for the scaling up of solid state fermentation systems[J].Biotechnology Reports, 2022, 36:e00764.
[25] MARTÍNEZ-ESPINOSA R M.Molecular advances in microbial metabolism 2.0[J].International Journal of Molecular Sciences, 2024, 25(2):1361.
[26] REN Z Q, CHEN Q X, TANG T X, et al.Unraveling the water source and formation process of Huangshui in solid-state fermentation[J].Food Science and Biotechnology, 2025, 34(3):665-675.
[27] SAARNI A, MILLER K V, BLOCK D E.A multi-parameter, predictive model of starch hydrolysis in barley beer mashes[J].Beverages, 2020, 6(4):60.
[28] RECEK N, ZHOU R W, ZHOU R S, et al.Improved fermentation efficiency of S.cerevisiae by changing glycolytic metabolic pathways with plasma agitation[J].Scientific Reports, 2018, 8(1):8252.
[29] CHEN C, XIONG Y, XIE Y H, et al.Metabolic characteristics of lactic acid bacteria and interaction with yeast isolated from light-flavor Baijiu fermentation[J].Food Bioscience, 2022, 50:102102.
[30] HUANG Y H, YI Z L, JIN Y L, et al.New microbial resource:Microbial diversity, function and dynamics in Chinese liquor starter[J].Scientific Reports, 2017, 7:14577.
[31] XUE T D, ZHANG J H, WANG T R, et al.Reveal the microbial communities and functional prediction during the fermentation of Fen-flavor Baijiu via metagenome combining amplicon sequencing[J].Annals of Microbiology, 2023, 73(1):16.
[32] 王雪山, 杜海, 徐岩.清香型白酒发酵过程中微生物种群空间分布[J].食品与发酵工业, 2018, 44(9):1-8.
WANG X S, DU H, XU Y.Spatial distribution of microbial community during Chinese light-flavor liquor fermentation[J].Food and Fermentation Industries, 2018, 44(9):1-8.
[33] WANG H Y, XU Y.Microbial succession and metabolite changes during the fermentation of Chinese light aroma-style liquor[J].Journal of the Institute of Brewing, 2019, 125(1):162-170.
[34] WANG H Y, GAO Y B, FAN Q W, et al.Characterization and comparison of microbial community of different typical Chinese liquor Daqus by PCR-DGGE[J].Letters in Applied Microbiology, 2011, 53(2):134-140.
[35] WANG H Y, XU Y.Effect of temperature on microbial composition of starter culture for Chinese light aroma style liquor fermentation[J].Letters in Applied Microbiology, 2015, 60(1):85-91.
[36] PANG X N, HAN B Z, HUANG X N, et al.Effect of the environment microbiota on the flavour of light-flavour Baijiu during spontaneous fermentation[J].Scientific Reports, 2018, 8:3396.
[37] WANG L L, WU L, XIANG D H, et al.Characterization of key aroma compounds in aged Qingxiangxing Baijiu by comparative aroma extract dilution analysis, quantitative measurements, aroma recombination, and omission studies[J].Food Chemistry, 2023, 419:136027.
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