食品与发酵工业 >

2025 , Vol. 51 >Issue 17: 296 - 304

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042853

研究报告

新疆樱桃李果皮花色苷的提取、纯化及成分分析

  • 谭峻杰 ,
  • 田占萍 ,
  • 西尔扎提·艾海提 ,
  • 吉舒仪 ,
  • 摆雅文 ,
  • 沈静 ,
  • 姚军
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  • 1(新疆医科大学 药学院,新疆 乌鲁木齐,830011)
    2(新疆医科大学第一附属医院 药学部,新疆药物临床研究重点实验室,新疆 乌鲁木齐,830054)
第一作者:硕士研究生(沈静教授和姚军教授为共同通信作者,E-mail:6572177@qq.com;xydyaojun@163.com)

收稿日期: 2025-03-26

  修回日期: 2025-04-29

  网络出版日期: 2025-09-29

基金资助

国家自然科学基金项目(82360827);中央引导地方科技发展专项资金项目(ZYYD2023B11);新疆医科大学研究生创新创业项目(CXCY2025022)

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Extraction, purification and composition analysis of anthocyanins from Xinjiang Prunus cerasifera Ehrh.peel

  • TAN Junjie ,
  • TIAN Zhanping ,
  • XIERZHATI Aihaiti ,
  • JI Shuyi ,
  • BAI Yawen ,
  • SHEN Jing ,
  • YAO Jun
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  • 1(School of Pharmacy, Xinjiang Medical University, Urumqi 830011, China)
    2(The First Affiliated Hospital of Xinjiang Medical University, Department of Pharmacy, Xinjiang Key Laboratory of Clinical Drug Research, Urumqi 830054, China)

Received date: 2025-03-26

  Revised date: 2025-04-29

  Online published: 2025-09-29

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摘要

为了促进新疆樱桃李果皮花色苷的资源利用,构建一种绿色环保的生产工艺,以樱桃李果皮为原料,制备高纯度的花色苷。以总花色苷含量为评价指标,用果胶酶辅助提取,通过单因素和正交试验优化新疆樱桃李果皮花色苷的提取工艺,并利用大孔吸附树脂-聚酰胺树脂联合纯化花色苷,借助超高效液相色谱-四级杆-飞行时间质谱(ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry, UPLC-Q-TOF/MS)对组分进行成分分析。结果表明,最佳提取条件为:果胶酶添加量0.2%、pH 2.0、料液比1:80(g:mL)、温度70 ℃、提取时间30 min。在此条件下总花色苷含量为1 718.35 mg/100 g。聚酰胺树脂纯化工艺的最佳条件为:上样液pH 3.0、上样体积10 BV、乙醇体积分数50%、洗脱体积3 BV、流速2.5 mL/min。纯化后总花色苷含量为50.72%。UPLC-Q-TOF/MS鉴定出30种成分,包括9种花色苷类、10种黄酮类、4种酚酸类和7种苯丙素类。该研究建立了“果胶酶辅助提取-双树脂联合纯化”体系,实现了花色苷的绿色提取与富集,结合化学成分鉴定结果,可为其资源的合理开发和药效物质基础提供科学依据。

关键词： 樱桃李; 花色苷; 提取; 纯化; 成分分析

本文引用格式

谭峻杰 , 田占萍 , 西尔扎提·艾海提 , 吉舒仪 , 摆雅文 , 沈静 , 姚军 . 新疆樱桃李果皮花色苷的提取、纯化及成分分析[J]. 食品与发酵工业, 2025 , 51(17) : 296 -304 . DOI: 10.13995/j.cnki.11-1802/ts.042853

Abstract

To enhance the resource utilization of anthocyanins from the peel of Xinjiang Prunus cerasifera Ehrh., an environmentally friendly production process has been developed.This process utilized the fruit peel as raw material to efficiently produce high-purity anthocyanins.Using the total anthocyanin content as the evaluation index, pectinase-assisted extraction was employed to optimize the extraction process through single-factor and orthogonal experiments.Macroporous adsorbent resin combined with polyamide resin was used for the purification of anthocyanins, and ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was employed for composition analysis.The results showed that the optimal extraction conditions were as follows:pectinase dosage of 0.2%, sample pH of 2.0, solid-to-liquid ratio of 1:80(g:mL), temperature of 70 ℃, and extraction time of 30 minutes.Under these conditions, the total anthocyanin content in the peel reached 1 718.35 mg/100 g.The optimal purification conditions using polyamide resin were as follows:sample pH of 3.0, sample volume of 10 BV, ethanol eluent concentration of 50%, elution volume of 3 BV, and flow rate of 2.5 mL/min.After purification, the total anthocyanin content reached 50.72%.UPLC-Q-TOF/MS analysis identified 30 components, including 9 anthocyanins, 10 flavonoids, 4 phenolic acids, and 7 phenylpropanoids.This study developed an integrated ‘pectinase-assisted extraction and dual-resin purification' system, which achieved green extraction and enrichment of anthocyanins.The combination of chemical composition analysis of P.cerasifera peel with this innovative methodology provides a scientific foundation for both sustainable resource utilization and identification of pharmacologically active constituents.

Key words： Prunus cerasifera Ehrh.; anthocyanins; extraction; purification; composition analysis

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