食品与发酵工业 >

2025 , Vol. 51 >Issue 17: 326 - 334

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041333

研究报告

海藻酸钠/玉米醇溶蛋白/1-氨基环丙烷-1-羧酸脱氨酶活性菌复合涂膜剂的制备及对兰州百合的保鲜效果

  • 周荣桃 ,
  • 黄伊宁 ,
  • 孟令煜 ,
  • 孙尔勤 ,
  • 晋玲 ,
  • 王引权
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  • 1(甘肃中医药大学 药学院,甘肃 兰州,730000)
    2(西北中藏药省部共建协同创新中心,甘肃 兰州,730000)
第一作者:硕士研究生(王引权教授和晋玲教授为共同通信作者,E-mail:kikfpp@163.com;zyxyj1@163.com)

收稿日期: 2024-10-18

  修回日期: 2024-12-26

  网络出版日期: 2025-09-29

基金资助

国家现代农业产业技术体系资助项目(CARS-21);中药保障与创新能力提升项目(甘中医药综函〔2024〕14号);甘肃省科技重大专项项目(23ZDFA013-1);甘肃省教育厅“双一流”科研重点项目(GSSYLXM05);西北中藏药省部共建协同创新中心项目(Xbzzy-2022-01)

收起

Preparation of sodium alginate/Zein/ACCD active bacteria composite coating agent and its preservation effect on Lanzhou lily

  • ZHOU Rongtao ,
  • HUANG Yining ,
  • MENG Lingyu ,
  • SUN Erqin ,
  • JIN Ling ,
  • WANG Yinquan
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  • 1(College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China)
    2(Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province & MOE of PRC, Lanzhou 730000, China)

Received date: 2024-10-18

  Revised date: 2024-12-26

  Online published: 2025-09-29

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摘要

为延长兰州百合的货架期,该研究从兰州百合根际土中筛选出具有1-氨基环丙烷-1-羧酸脱氨酶(1-aminocyclopropane-1-carboxylic acid deaminase,ACCD)活性的菌株,利用ACCD能够降解植物内源乙烯的特性,采用包埋技术,以海藻酸钠(sodium alginate,SA)和玉米醇溶蛋白为基材制备出一种复合涂膜剂并进行工艺优化,同时探究了该涂膜剂的特征及保鲜性能。结果表明,氧化微杆菌(Microbacterium oxydans)的安全性较好且ACCD活性最高,可达(0.119±0.006) μmol/(mg·h)。优化并根据实际情况修正后膜剂的最佳工艺为SA 3.4%(质量分数)、甘油添加量22.8%、CaCl2 2.8%(质量分数)。在此工艺条件下,测得膜剂的菌株包埋率为60.56%,其结构表征结果与性能相互印证。与未涂膜组相比,该膜剂可有效降低兰州百合鳞茎片的褐变度、减缓呼吸速率和丙二醛含量、并抑制过氧化物酶、多苯丙氨酸解氨酶的活性。且在同处理条件下的4 ℃具有较优的保鲜效果。该研究成功制备出具有良好的形貌、性能及保鲜效果的复合涂膜剂,为兰州百合保鲜新工艺的开发提供了一定理论依据。

关键词： 兰州百合; 海藻酸钠; 玉米醇溶蛋白; 1-氨基环丙烷-1-羧酸脱氨酶活性菌; 涂膜剂; 保鲜

本文引用格式

周荣桃 , 黄伊宁 , 孟令煜 , 孙尔勤 , 晋玲 , 王引权 . 海藻酸钠/玉米醇溶蛋白/1-氨基环丙烷-1-羧酸脱氨酶活性菌复合涂膜剂的制备及对兰州百合的保鲜效果[J]. 食品与发酵工业, 2025 , 51(17) : 326 -334 . DOI: 10.13995/j.cnki.11-1802/ts.041333

Abstract

In order to prolong the shelf life of Lanzhou lily [Lilium davidii var.willmottiae (E.H.Wilson) Raffill], this study screened a strain with 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) activity from the rhizosphere soil of Lanzhou lily.Based on the characteristics of ACCD that can degrade endogenous ethylene in plants, a composite coating agent was prepared by embedding technology with sodium alginate (SA) and Zein as substrates, and the process was optimized.At the same time, the characteristics and fresh-keeping properties of the coating agent were explored.The results showed that Microbacterium oxydans had good safety and the highest ACCD activity, which could reach (0.119±0.006) μmol/(mg·h).After optimization and modification according to the actual situation, the best process for the film agent was 3.4% (mass fraction) SA, 22.8% Gl addition, and 2.8% (mass fraction) CaCl2.Under this technological condition, the strain embedding rate of the film agent was 60.56%, and its structure characterization results and performance were mutually confirmed.Compared with the uncoated film group, the film could effectively reduce the browning degree of bulb slices of Lanzhou lily, slow down the respiration rate and malondialdehyde content, and inhibit the activities of peroxidase and polyphenylalanine ammonia lyase enzymes.Moreover, it has a better fresh-keeping effect at 4 ℃ under the same treatment conditions.In this study, a composite coating agent with good morphology, properties, and fresh-keeping effect was successfully prepared, which provided a theoretical basis for the development of new fresh-keeping technology for Lanzhou lily.

Key words： Lilium davidii var.willmottiae (E.H.Wilson) Raffill; sodium alginate; Zein; ACCD active bacteria; film coating agent; preservation

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