研究报告

不同青稞麦芽添加量对啤酒理化指标、多酚含量、抗氧化活性及风味的影响

  • 王敏 ,
  • 白术群 ,
  • 郑学玲
展开
  • 1(河南工业大学 粮油食品学院,河南 郑州,450001)
    2(中粮营养健康研究院有限公司,营养健康与食品安全北京市重点实验室,北京,102200)
第一作者:硕士研究生(白术群正高级工程师和郑学玲教授为共同通信作者,E-mail:baishuqun@ cofco.com;xuelingzheng@haut.edu.cn)

收稿日期: 2024-11-11

  修回日期: 2025-01-13

  网络出版日期: 2025-10-16

基金资助

国家小麦产业技术体系专项资金资助项目(CARS-03-38)

Effects of different amounts of highland barley malt on physical and chemical indexes, polyphenols content, antioxidant activity and flavor of beer

  • WANG Min ,
  • BAI Shuqun ,
  • ZHENG Xueling
Expand
  • 1(School of Grain and Oil Food, Henan University of Technology, Zhengzhou 450001, China)
    2(Beijing Key Laboratory of Nutrition, Health & Food Safety, Beijing Engineering Laboratory of Geriatric Nutrition & Food, Nutrition & Health Research Institute, COFCO Corporation, Beijing 102200, China)

Received date: 2024-11-11

  Revised date: 2025-01-13

  Online published: 2025-10-16

摘要

为探究青稞麦芽在啤酒酿造中的应用价值及其对啤酒品质的影响,以不同添加比例的青稞麦芽(甘青4号)与大麦麦芽(0、20%、40%、60%、80%、100%,质量分数)为原料酿造青稞啤酒,并对其进行分析。采用高效液相色谱法测定酒精含量,福林酚法测定总酚含量,ABTS、DPPH法测定抗氧化能力,水杨酸法测定羟自由基清除能力,铁离子抗氧化能力法测定总抗氧化能力,采用顶空固相微萃取-气相色谱-质谱联用技术测定风味物质含量。结果表明,添加青稞麦芽显著增加了啤酒的浊度、色度、酚类物质含量、β-葡聚糖含量和抗氧化活性,降低了啤酒的发酵度、乙醇含量和pH;风味物质分析结果表明,添加80%(质量分数)青稞麦芽酿造的啤酒风味物质种类最多,为40种; 添加100%(质量分数)青稞麦芽酿造的啤酒风味物质含量最多,为91.17 μg/mL;主成分分析结果显示,不同比例青稞啤酒位于得分图的不同象限,表明各组风味物质可以被前2个主成分区分;感官分析结果表明,添加60%(质量分数)青稞麦芽酿造的啤酒口感最好。总体而言,添加青稞麦芽对提高啤酒品质及风味有积极作用,可成为未来多元化啤酒产品开发的重要方向。

本文引用格式

王敏 , 白术群 , 郑学玲 . 不同青稞麦芽添加量对啤酒理化指标、多酚含量、抗氧化活性及风味的影响[J]. 食品与发酵工业, 2025 , 51(18) : 118 -126 . DOI: 10.13995/j.cnki.11-1802/ts.041557

Abstract

To explore the application value of highland barley malt in beer brewing and its effect on beer quality, highland barley beer was brewed with different proportions of highland barley malt (Gan Qing No.4) and barley malt (0, 20%, 40%, 60%, 80%, 100%, mass fraction) as raw materials, and it was analyzed.The alcohol content was determined by high performance liquid chromatography, the total phenolic content was determined by Folin-ciocalteu, the antioxidant capacity was determined by ABTS and DPPH methods, the hydroxyl radical scavenging capacity was determined by salicylic acid method, the total antioxidant capacity was determined by FRAP method, and headspace solid phase microextraction-gas was used by headspace solid phase microextraction and gas chromatography chromatography-mass spectrometry (HS-SPME-GC-MS) to determine the flavor substance content.The results showed that adding highland barley malt significantly increased the turbidity, chromaticity, phenolic content, β-glucan content, and antioxidant activity of beer, and decreased the fermentation degree, alcohol content, and pH value of beer.The results of flavor substance analysis showed that the beer with 80% (mass fraction) highland barley malt had the largest variety of flavor substances, with 40 kinds. The beer with 100% (mass fraction) highland barley malt had the highest flavor content of 91.17 μg/mL.PCA analysis indicated that different proportions of highland barley beer were located in different quadrants of the score chart, indicating that each flavor substance could be distinguished by the first two principal components.The results of sensory analysis showed that the beer brewed with 60% (mass fraction) highland barley malt had the best taste.Overall, the addition of highland barley malt plays a positive role in improving the quality and flavor of beer and can become an important direction of diversified beer product development in the future.

参考文献

[1] GUO T L, HORVATH C, CHEN L, et al. Understanding the nutrient composition and nutritional functions of highland barley (Qingke): A review[J]. Trends in Food Science & Technology, 2020, 103:109-117.
[2] 刘皓, 陈烨, 曾英杰, 等. 不同比例黑青稞麦芽汁中酵母的发酵性能及啤酒品质[J]. 现代食品科技, 2024, 40(3):74-82.
LIU H, CHEN Y, ZENG Y J, et al. Fermentation performance of yeast in malt wort with different proportions of black highland barley and the resulting beer quality[J]. Modern Food Science and Technology, 2024, 40(3):74-82.
[3] 于佳俊, 郝建秦, 皇甫洁. 青稞麦芽酿造特性及产品功能性研究[J]. 大麦与谷类科学, 2018, 35(4):59.
YU J J, HAO J Q, HUANGFU J. Study on brewing characteristics and product functionality of hulless barley malt[J]. Barley and Cereal Sciences, 2018, 35(4):59.
[4] AO F, ZHENG J, WU J H, et al. Effect of mashing temperature on fermentation and antioxidant capacity of Qingke wort[J]. Cereal Chemistry, 2024, 101(1):206-219.
[5] QUIFER-RADA P, VALLVERDÚ-QUERALT A, MARTÍNEZ-HUÉLAMO M, et al. A comprehensive characterisation of beer polyphenols by high resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-MS)[J]. Food Chemistry, 2015, 169:336-343.
[6] HABSCHIED K, LONČARIĆ A, MASTANJEVIĆ K. Screening of polyphenols and antioxidative activity in industrial beers[J]. Foods, 2020, 9(2):238.
[7] 叶海生. β-葡聚糖对啤酒酿造过程的影响及控制[J]. 啤酒科技, 2007(12):47-48.
YE H S. Effect and control of β-glucan on beer brewing process[J]. Beer Science and Technology, 2007(12):47-48.
[8] 弭孝涛, 吕爱霞, 耿靖玮, 等. 上面发酵青稞啤酒糖化工艺研究[J]. 中国酿造, 2010, 29(11):73-75.
MI X T, LYU A X, GENG J W, et al. Saccharification of top fermentation hulless barley(Qing Ke) beer[J]. China Brewing, 2010, 29(11):73-75.
[9] 李慧敏. 上面发酵和下面发酵小麦啤酒的差别研究[D].淄博: 山东理工大学, 2012.
LI H M. Differences research on top and bottom fermented wheat beer [D]. Zibo: Shandong University of Technology, 2012.
[10] 陈文波, 张一, 李小燕, 等. 威士忌制备过程中理化指标及挥发性风味物质动态变化规律研究[J]. 中国酿造, 2024, 43(2):88-97.
CHEN W B, ZHANG Y, LI X Y, et al. Dynamic changes rules of physicochemical indexes and volatile flavor compounds during whisky preparation process[J]. China Brewing, 2024, 43(2):88-97.
[11] 樊玉婷, 李智昊, 李月垣, 等. 不同品种葡萄添加量对果啤香气、理化指标及抗氧化活性的影响[J]. 食品科学, 2024, 45(13):164-172.
FAN Y T, LI Z H, LI Y Y, et al. Effects of addition of different amounts and varieties of grapes on aroma, physicochemical indexes and antioxidant activity of fruit beer[J]. Food Science, 2024, 45(13):164-172.
[12] 李玉栋. 麦麸不溶性膳食纤维改性及对面条品质的影响[D].郑州: 河南工业大学, 2023.
LI Y D. Modification of wheat bran insoluble dietary fiber and its effect on noodle quality[D]. Zhengzhou: Henan University of Technology,2023.
[13] GIANNETTI V, BOCCACCI MARIANI M, TORRELLI P, et al. Flavour component analysis by HS-SPME/GC-MS and chemometric modeling to characterize Pilsner-style Lager craft beers[J]. Microchemical Journal, 2019, 149:103991.
[14] VIEIRA A C, PEREIRA A C, MARQUES J C, et al. Multi-target optimization of solid phase microextraction to analyse key flavour compounds in wort and beer[J]. Food Chemistry, 2020, 317:126466.
[15] VAN GEMERT L J. Compilations of Odour Threshold Values in Air, Water and Other Media[M]. Netherlands:Oliemans Punter & Partners BV, 2003.
[16] ZHAO M, LI T, YANG F, et al. Characterization of key aroma-active compounds in Hanyuan Zanthoxylum bungeanum by GC-O-MS and switchable GC×GC-O-MS[J]. Food Chemistry, 2022, 385:132659.
[17] 陈祖乙, 安明哲, 李丽, 等. 青稞精酿啤酒酿造工艺优化[J]. 中国酿造, 2022, 41(4):174-179.
CHEN Z Y, AN M Z, LI L, et al. Optimization for brewing process of highland barley craft beer[J]. China Brewing, 2022, 41(4):174-179.
[18] 梁海燕. β-葡聚糖在啤酒酿造过程中的变化及其影响[J]. 啤酒科技, 2004(12):44-45.
LIANG H Y. Changes and effects of β-glucan in beer brewing process[J]. Beer Science and Technology, 2004(12):44-45.
[19] 张尧. 影响啤酒发酵度关键因素的分析研究[D].太原: 山西农业大学, 2016.
ZHANG Y. Research on the key factors of fermentation degree[D]. Taiyuan: Shanxi Agricultural University, 2016.
[20] 徐菲, 党斌, 杨希娟, 等. 不同青稞品种的营养品质评价[J]. 麦类作物学报, 2016, 36(9):1249-1257.
XU F, DANG B, YANG X J, et al. Evaluation of nutritional quality of different hulless barleys[J]. Journal of Triticeae Crops, 2016, 36(9):1249-1257.
[21] YANG H R, COLDEA T E, ZENG Y J, et al. Wheat gluten hydrolysates promotes fermentation performance of brewer’s yeast in very high gravity worts[J]. Bioresources and Bioprocessing, 2021, 8(1):5.
[22] 管敦仪. 啤酒工业手册[M].北京: 中国轻工业出版社, 1998.
GUAN D Y. Beer Industry Handbook[M]. Beijing: China Light Industry Press, 1998.
[23] 王小平, 雷激, 唐诗, 等. 酵母发酵改善麸皮食用品质的研究[J]. 食品工业科技, 2016, 37(10):231-235; 241.
WANG X P, LEI J, TANG S, et al. Improving the edible quality of the bran by yeast fermentation[J]. Science and Technology of Food Industry, 2016, 37(10):231-235; 241.
[24] 于洪梅, 赵寿经, 王妮. 低双乙酰啤酒酵母菌种的诱变及对啤酒酿造的影响[J]. 吉林大学学报(工学版), 2021, 51(5):1919-1925.
YU H M, ZHAO S J, WANG N. Screening of brewer’s yeast with low yield of higher alcohols and the effect on beer brewing technology[J]. Journal of Jilin University (Engineering and Technology Edition), 2021, 51(5):1919-1925.
[25] PIECZONKA S A, HEMMLER D, MORITZ F, et al. Hidden in its color: A molecular-level analysis of the beer’s Maillard reaction network[J]. Food Chemistry, 2021, 361:130112.
[26] WANG L M, HONG K, AGBAKA J I, et al. Characterization of bitter-tasting and antioxidant activity of dry-hopped beers[J]. Journal of the Science of Food and Agriculture, 2022, 102(11):4843-4853.
[27] FLOEGEL A, KIM D O, CHUNG S J, et al. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods[J]. Journal of Food Composition and Analysis, 2011, 24(7):1043-1048.
[28] VILJANEN K, LILLE M, HEINIÖ R L, et al. Effect of high-pressure processing on volatile composition and odour of cherry tomato purée[J]. Food Chemistry, 2011, 129(4):1759-1765.
[29] 卞小稳. 荞麦在啤酒酿造中的应用研究[D].无锡: 江南大学, 2016.
BIAN X W.Study on Application of Buckwheat in the Beer Brewing[D]. Wuxi: Jiangnan University, 2016.
[30] 张一, 李小燕, 牛丽敏, 等. 4款单一麦芽威士忌特征风味成分解析[J]. 食品科学, 2024, 45(5):111-117.
ZHANG Y, LI X Y, NIU L M, et al. Analysis of key aroma components of four kinds of single malt whisky[J]. Food Science, 2024, 45(5):111-117.
[31] 陈丹花. 小麦在啤酒酿造中的应用研究[D].无锡: 江南大学, 2008.
CHEN D H.Study on application of wheat in the brewing of light beer[D]. Wuxi: Jiangnan University,2008.
[32] 田金凤, 王金晶, 郑飞云, 等. 啤酒主要组分在泡沫中的富集及其对泡持性的影响[J]. 食品与发酵工业, 2017, 43(9):22-27.
TIAN J F, WANG J J, ZHENG F Y, et al. Analysis on enrichment of main components of beer in beer foam and its effect on foam retention[J]. Food and Fermentation Industries, 2017, 43(9):22-27.
[33] 韩龙. 淡爽型啤酒风味协调性控制[J]. 中外酒业·啤酒科技, 2017(7):46-48.
HAN L. Flavor coordination control of light beer[J]. Global Alcinfo, 2017(7):46-48.
文章导航

/