食品与发酵工业 >

2025 , Vol. 51 >Issue 20: 44 - 52

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041922

研究报告

壳寡糖界面吸附顺序及浓度对多层乳液热稳定及DHA缓释保护的影响

  • 陈玉峰 ,
  • 丁嘉悦 ,
  • 孙怡 ,
  • 周绪霞 ,
  • 刘书来 ,
  • 丁玉庭
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  • 1(浙江工业大学 食品科学与工程学院,浙江 杭州,310014)
    2(全省深蓝渔业资源绿色低碳高效开发重点实验室,浙江 杭州,310014)
第一作者:博士,副教授(丁玉庭教授为通信作者,E-mail:dingyt@zjut.edu.cn)

收稿日期: 2024-12-21

  修回日期: 2025-03-07

  网络出版日期: 2025-10-27

基金资助

国家自然科学基金面上项目(32172242);国家自然科学基金青年项目(32402098)

收起

Study on effects of chitosan oligosaccharide interface adsorption sequence and concentration on thermal stability and DHA sustained-release protection of multilayer emulsions

  • CHEN Yufeng ,
  • DING Jiayue ,
  • SUN Yi ,
  • ZHOU Xuxia ,
  • LIU Shulai ,
  • DING Yuting
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  • 1(College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China)
    2(Zhejiang Key Laboratory of Green, Low-carbon and Efficient Development of Marine Fishery Resources, Hangzhou 310014, China)

Received date: 2024-12-21

  Revised date: 2025-03-07

  Online published: 2025-10-27

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摘要

该研究探讨微射流高压(dynamic high pressure microfluidization,DHPM)-谷氨酰胺转氨酶(transglutaminase,TG)协同处理的改性乳清蛋白(DHPM-TG-whey protein concentrate,DHPM-TG-WPC)与壳寡糖(chitosan oligosaccharide,COS)的界面吸附顺序及浓度对藻油多层乳液热稳定性和二十二碳六烯酸(docosahexaenoic acid,DHA)缓释保护的影响。结果表明,“DHPM-TG-WPC-低酯果胶(low-methoxy pectin,LMP)-COS”顺序通过静电相互作用和共价交联作用形成稳定界面层,显著提升热稳定性;而“DHPM-TG-WPC-COS-LMP”顺序因界面层不稳定导致液滴聚集和结构破坏。COS浓度显著影响乳液性能,5 g/L COS可增强界面层强度,减少液滴聚集,提高稳定性;但10 g/L COS因吸附不均引发絮凝加剧,稳定性下降。在模拟消化中,5 g/L COS通过减少脂肪酶与油滴接触延缓DHA水解;而10 g/L COS在碱性肠液中易脱落,导致界面层失稳并加速DHA释放。该研究揭示了COS界面吸附顺序及浓度对多层乳液稳定性和DHA缓释的调控机制,为DHA功能性乳液优化设计提供了理论依据。

关键词： 多层乳液; 藻油; 壳寡糖; 热稳定性; DHA缓释

本文引用格式

陈玉峰 , 丁嘉悦 , 孙怡 , 周绪霞 , 刘书来 , 丁玉庭 . 壳寡糖界面吸附顺序及浓度对多层乳液热稳定及DHA缓释保护的影响[J]. 食品与发酵工业, 2025 , 51(20) : 44 -52 . DOI: 10.13995/j.cnki.11-1802/ts.041922

Abstract

This study investigated the effects of the interfacial adsorption sequence and concentration of micro-fluidized high-pressure (DHPM)-transglutaminase (TG) co-treated modified whey protein concentrate (DHPM-TG-WPC) and chitosan oligosaccharide (COS) on the thermal stability and docosahexaenoic acid (DHA) sustained-release protection of algae oil multilayer emulsions.Results demonstrated that the “DHPM-TG-WPC-low methoxyl pectin-COS” sequence formed a stable interfacial layer through electrostatic interactions and covalent cross-linking, significantly enhancing thermal stability.In contrast, the “DHPM-TG-WPC-COS-LMP” sequence led to droplet aggregation and structural disruption due to interfacial instability.COS concentration markedly influenced emulsion performance, 5 g/L COS strengthened the interfacial layer, reduced droplet aggregation, and improved stability, whereas 10 g/L COS caused flocculation and stability degradation due to uneven adsorption.In simulated digestion, 5 g/L COS delayed DHA hydrolysis by reducing lipase-oil droplet contact, while 10 g/L COS was prone to detachment in alkaline intestinal fluid, destabilizing the interfacial layer and accelerating DHA release.This study elucidated the regulatory mechanisms of COS interfacial adsorption sequence and concentration on multilayer emulsion stability and DHA sustained release, providing a theoretical basis for the optimized design of DHA-functional emulsions.

Key words： multilayer emulsions; algal oil; chitosan oligosaccharides; thermal stability; DHA sustained release

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