食品与发酵工业 >

2025 , Vol. 51 >Issue 20: 93 - 100

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041772

研究报告

冷应激对冷冻干燥鼠李糖乳酪杆菌B6活性的保护作用

  • 杨婷 ,
  • 张佳 ,
  • 张娟 ,
  • 吴正钧
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  • 1(光明乳业股份有限公司乳业研究院,上海乳业生物工程技术研究中心,乳业生物技术国家重点实验室,上海,200436)
    2(上海大学 生命科学学院,上海,200444)
第一作者:博士(吴正钧教授级高级工程师为通信作者,E-mail:wuzhengjun@brightdairy.com)

收稿日期: 2024-12-04

  修回日期: 2025-03-10

  网络出版日期: 2025-10-27

基金资助

十四五国家重点研发计划(2022YFD2100704);市国资委企业创新发展与能级提升项目(2022013)

收起

Protective effect of cold stress on activity of freeze-dried Lacticaseibacillus rhamnosus B6

  • YANG Ting ,
  • ZHANG Jia ,
  • ZHANG Juan ,
  • WU Zhengjun
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  • 1(State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co.Ltd., Shanghai 200436, China)
    2(College of Life Science, Shanghai University, Shanghai 200444, China)

Received date: 2024-12-04

  Revised date: 2025-03-10

  Online published: 2025-10-27

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摘要

为探究冷应激对冷冻干燥乳酸菌活性的影响,通过以鼠李糖乳酪杆菌(Lacticaseibacillus rhamnosus)B6为研究对象,考察7种保护剂和不同冷应激处理对其冷冻干燥的保护效果,以及探讨了在不同冷应激温度及时间下其存活率、酸化能力、乳酸脱氢酶及细胞膜完整性的变化规律。结果表明,100 g/L脱脂乳为最佳保护剂,其冷冻存活率达到了79.30%,显著高于其他保护剂的存活率(P<0.05)。在100 g/L脱脂乳为保护剂的基础上,4 ℃冷应激2 h时冷冻干燥存活率能够达到(97.94±1.1)%,较未冷应激对照组的存活率提高了18.64%,并且优于其他冷应激的条件(P<0.05)。此外,4 ℃冷应激2 h能够显著提高L.rhamnosus B6冷冻干燥后的酸化能力、乳酸脱氢酶的活性,以及维持细胞膜完整性。因此,该研究表明冷应激处理方法对冷冻L.rhamnosus的活力具有有效的保护效果。

关键词： 冷应激; 冷冻干燥; 鼠李糖乳酪杆菌; 存活率; 酸化

本文引用格式

杨婷 , 张佳 , 张娟 , 吴正钧 . 冷应激对冷冻干燥鼠李糖乳酪杆菌B6活性的保护作用[J]. 食品与发酵工业, 2025 , 51(20) : 93 -100 . DOI: 10.13995/j.cnki.11-1802/ts.041772

Abstract

To explore the impact of cold stress on the activity of freeze-dried lactic acid bacteria, Lacticaseibacillus rhamnosus B6 was selected as the research subject.Seven protectants and various cold stress treatments were evaluated for their protective effects during freeze-drying.Additionally, this study explored the patterns of changes in survival rate, acidification capacity, lactate dehydrogenase activity, and cell membrane integrity of strain B6 under various cold stress temperature and durations.Results indicated that 100 g/L skim milk was the optimal protectant, achieving a high freeze survival rate of 79.30%.This survival rate was significantly higher than that of other protectants (P<0.05).With 100 g/L skimmed milk as protective agent, the survival rate of freeze-drying could reach (97.94±1.1)% under cold stress at 4 ℃ for 2 h.Compared with the group without cold stress treatment, the survival rate of cold stress at 4 ℃ for 2 h increased by 18.64%.Moreover, the effect of this cold stress condition was significantly better than other cold stress conditions (P<0.05).Furthermore, results showed that cold stress at 4 ℃ for 2 h could significantly improve the acidification ability and lactate dehydrogenase activity of L.rhamnosus B6 after freeze-drying, and maintain the integrity of cell membrane.Therefore, this study demonstrated that cold stress treatment was an effective method for preserving the viability of freeze-dried L.rhamnosus.

Key words： cold stress; freeze-drying; Lacticaseibacillus rhamnosus; survival rate; acidification

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