食品与发酵工业 >

2025 , Vol. 51 >Issue 20: 181 - 189

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041815

研究报告

厌氧处理对桑红茶活性成分及其抗氧化活性的影响

  • 夏文银 ,
  • 吴劲轩 ,
  • 殷浩 ,
  • 夏川林 ,
  • 王香君
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  • (四川省农业科学院蚕业研究所,四川省农业科学院特种经济动植物研究所,四川 南充,637000)
第一作者:硕士研究生,助理研究员(殷浩研究员为通信作者,E-mail:s20060428@163.com)

收稿日期: 2024-12-10

  修回日期: 2025-03-06

  网络出版日期: 2025-10-27

基金资助

四川省中央引导地方科技发展专项项目(2024ZYD0306);南充市科技计划项目(23XCZX0026);四川省农业科学院“1+9”揭榜挂帅科技攻关项目(1+9KJGG007);四川省财政自主创新专项(2022ZZCX089);四川省科技创新人才项目(2023 JDRC0038)

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Effects of anaerobic treatment on the active ingredient and antioxidant activity of mulberry leaf black tea

  • XIA Wenyin ,
  • WU Jinxuan ,
  • YIN Hao ,
  • XIA Chuanlin ,
  • WANG Xiangjun
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  • (Sericulture Research Institute of Sichuan Academy of Agricultural Sciences, nstitute of Special Economic Flora and Fauna of Sichuan Academy of Agricultural Sciences, Nanchong 637000, China)

Received date: 2024-12-10

  Revised date: 2025-03-06

  Online published: 2025-10-27

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摘要

为了改善桑叶红茶的功能特性,探究不同厌氧处理对桑叶红茶中γ-氨基丁酸(γ-aminobutyric acid, GABA)含量及其他活性成分的影响,并分析其体外抗氧化活性。结果表明,厌氧处理能显著提高桑叶红茶GABA含量,尤其是厌氧/好氧交替处理,能显著提高谷氨酸脱羧酶活性,促使谷氨酸脱羧生成GABA。厌氧处理4 h后,桑红茶GABA含量均达到Gabaron茶标准,而在厌氧/好氧交替处理3次时GABA含量达到最高(1.97±0.02) mg/g,是对照组的1.68倍,游离氨基酸、总黄酮、茶多酚含量比对照组分别提高了38.99%、25.83%、20.36%,可溶性糖和水浸出物分别下降了16.89%和8.57%,抗氧化活性ABTS阳离子自由基、DPPH自由基、羟自由基清除率、铁离子还原能力分别是对照组的2.52倍、2.29倍、1.84倍、1.03倍,相关性分析发现,茶多酚与羟自由基清除率相关性最高,GABA含量与DPPH自由基清除能力和铁离子还原能力相关性最高。该研究为高含量GABA功能桑茶的研究与开发提供理论参考。

关键词： 厌氧处理; 桑叶红茶; γ-氨基丁酸; 活性成分; 抗氧化活性

本文引用格式

夏文银 , 吴劲轩 , 殷浩 , 夏川林 , 王香君 . 厌氧处理对桑红茶活性成分及其抗氧化活性的影响[J]. 食品与发酵工业, 2025 , 51(20) : 181 -189 . DOI: 10.13995/j.cnki.11-1802/ts.041815

Abstract

This study aimed to improve the functional properties of mulberry leaf black tea.The effects of different anaerobic treatments on the content of γ-aminobutyric acid (GABA) and other active ingredients in mulberry leaf black tea, as well as its antioxidant activity, were investigated.The results showed that anaerobic processing significantly increased the GABA content in mulberry leaf black tea, especially the anaerobic/aerobic alternating treatment, which could significantly enhance the activity of glutamate decarboxylase and facilitate the decarboxylation of glutamate into GABA.After 4 hours of anaerobic treatment, the GABA content in mulberry leaf black tea exceeded that of Gabaron tea.Moreover, the highest GABA content was found in mulberry leaf black tea treated with three cycles of anaerobic/aerobic alternation (1.97±0.02) mg/g, which was 1.68 times that of the control group.The content of free amino acids, total flavonoids, and polyphenols in mulberry leaf black tea treated with three cycles of anaerobic/aerobic alternation increased by 38.99%, 25.83%, and 20.36%, separately, while the content of soluble sugars and water extracts decreased by 16.89% and 8.57% in comparison with the control group.In addition, the scavenging rate of ABTS cation free radical, DPPH radical, hydroxyl radical, and iron ion-reducing power of mulberry tea treated with three cycles of anaerobic/aerobic alternation were 2.52, 2.29, 1.84, and 1.03 times higher than that of the control group, separately.Correlation analysis revealed that polyphenol content exhibited the strongest correlation with hydroxyl radical scavenging capacity, while GABA content showed the highest correlation with DPPH free radical scavenging capacity and iron ion reducing power.This study provides a theoretical reference for the development of GABA-enriched functional mulberry tea.

Key words： anaerobic treatment; mulberry leaf black tea; γ-aminobutyric acid; active ingredient; antioxidant activity

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