食品与发酵工业 >

2025 , Vol. 51 >Issue 20: 224 - 231

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042181

研究报告

壳聚糖/乳酸菌无细胞上清液复合抗菌膜的制备及性能

  • 袁国惠 ,
  • 赵梓程 ,
  • 邓艳梅 ,
  • 杨通琴 ,
  • 高宝宇 ,
  • 郭磊 ,
  • 杨娇娇 ,
  • 范方宇
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  • 1(西南林业大学 生物与食品工程学院,云南 昆明,650224)
    2(西南地区生物多样性保育国家林业和草原局重点实验室,云南 昆明,650224)
    3(云南省森林灾害预警与控制实验室,云南 昆明,650224)
第一作者:硕士研究生(范方宇教授为通信作者,E-mail:ffy118@163.com)

收稿日期: 2025-01-17

  修回日期: 2025-03-19

  网络出版日期: 2025-10-27

基金资助

云南省重大科技专项计划项目(202302AE090019);云南省科技厅中青年学术和技术带头人后备人才项目(202405AC350032)

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Preparation and properties of chitosan/lactic acid bacteria cell-free supernatant composite antibacterial film

  • YUAN Guohui ,
  • ZHAO Zicheng ,
  • DENG Yanmei ,
  • YANG Tongqin ,
  • GAO Baoyu ,
  • GUO Lei ,
  • YANG Jiaojiao ,
  • FAN Fangyu
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  • 1(College of Biological and Food Engineering, Southwest Forestry University, Kunming 650224, China)
    2(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China)
    3(Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, China)

Received date: 2025-01-17

  Revised date: 2025-03-19

  Online published: 2025-10-27

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摘要

为提高包装膜的抗菌性,研究了不同比例的壳聚糖(chitosan,CS)、乳酸菌无细胞上清液(cell-free supernatant,CFS)复合保鲜液的抗菌、抗氧化性能,并以CS为成膜基质,制备CS与CFS不同体积比(15∶0、10∶1、10∶2、10∶3、10∶4、10∶5)的复合包装膜,探究CFS不同添加比例对复合膜形貌、结构、机械性能、阻隔性能等的影响。结果表明,复合保鲜液的抗菌和抗氧化效果与CFS的添加量呈正比,在CS和CFS为10∶5时达最大值,复合保鲜液对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌的抑菌圈直径分别为14.68、15.83和13.40 mm,DPPH自由基、ABTS阳离子自由基清除率分别为94.46%和92.24%。随着CFS含量的增加,复合膜的光阻隔性优于纯CS膜,其厚度、水蒸气透过率、溶解性、水分含量增加,CO2透过率降低。CS和CFS为10∶3时复合膜的机械性能和热稳定性最好,与纯CS膜相比,其拉伸强度和断裂伸长率分别提高了36.77%和70.94%(P＜0.05),熔融温度达125.28 ℃。表征分析表明复合膜具有良好的相容性,结构致密,稳定性较好。

关键词： 乳酸菌; 壳聚糖; 无细胞上清液; 复合膜; 抗菌

本文引用格式

袁国惠 , 赵梓程 , 邓艳梅 , 杨通琴 , 高宝宇 , 郭磊 , 杨娇娇 , 范方宇 . 壳聚糖/乳酸菌无细胞上清液复合抗菌膜的制备及性能[J]. 食品与发酵工业, 2025 , 51(20) : 224 -231 . DOI: 10.13995/j.cnki.11-1802/ts.042181

Abstract

To improve the antibacterial properties of packaging films, the antibacterial and antioxidant properties of composite preservative solution made from varying ratios of chitosan (CS) and cell-free supernatant (CFS) of lactic acid bacteria were investigated.Composite packaging films were prepared by varying the volume ratios of CS to CFS (15∶0, 10∶1, 10∶2, 10∶3, 10∶4, 10∶5) were prepared using CS as the film-forming matrix.The effects of different CFS ratios on the morphology, structure, mechanical properties, and barrier properties of the films were evaluated.The antibacterial and antioxidant effects of the composite preservative solution were positively proportional to the additional amount of CFS, reaching their maximum values at a CS to CFS ratio of 10∶5.The inhibition zone diameters against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was 14.68, 15.83, and 13.40 mm, respectively.DPPH free radical and ABTS cationic radical scavenging rates were 94.46% and 92.24%.With the increase in CFS content, the composite film exhibited improved light barrier properties compared to the pure CS film, along with increased thickness, water vapor permeability, water solubility, and water content, while CO2 permeability decreased.The mechanical properties and thermal stability of the composite film were optimal at a CS to CFS ratio of 10∶3.The tensile strength and elongation at break increased by 36.77% and 70.94% compared with the pure CS film (P＜0.05), and the melting temperature reached 125.28 ℃.Characterization analysis showed that the composite film exhibited excellent compatibility, dense structure, and enhanced stability.

Key words： lactic acid bacteria; chitosan; cell-free supernatant; composite film; antibacterial

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