食品与发酵工业 >

2025 , Vol. 51 >Issue 20: 239 - 246

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040691

研究报告

谷氨酰胺肽对乙醇诱导的胃黏膜损伤的保护作用研究

  • 许润琦 ,
  • 王憬 ,
  • 孟德豪 ,
  • 张海欣 ,
  • 马永庆 ,
  • 毕园 ,
  • 徐国阳 ,
  • 陈亮 ,
  • 方磊
展开
  • 1(中国食品发酵工业研究院有限公司,北京,100015)
    2(浙江省农村实业发展有限公司,浙江 杭州,310005)
第一作者:硕士,工程师(方磊工程师和陈亮正高级工程师为共同通信作者,E-mail:wewe359@163.com;269462959@qq.com)

收稿日期: 2024-08-08

  修回日期: 2025-03-11

  网络出版日期: 2025-10-27

基金资助

宁夏回族自治区重点研发计划项目(2021BEG02027)

收起

Study on the protective effect of glutamine peptides on ethanol-induced gastric mucosal injury

  • XU Runqi ,
  • WANG Jing ,
  • MENG Dehao ,
  • ZHANG Haixin ,
  • MA Yongqing ,
  • BI Yuan ,
  • XU Guoyang ,
  • CHEN Liang ,
  • FANG Lei
Expand
  • 1(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing 100015, China)
    2(Zhejiang Rural Industry Development Co.Ltd., Hangzhou 310005, China)

Received date: 2024-08-08

  Revised date: 2025-03-11

  Online published: 2025-10-27

Fold

摘要

该文探究谷氨酰胺肽对GES-1细胞在酒精损伤下的修复效应。首先,以分子质量分布为指标,研究了体外胃肠道模拟消化对谷氨酰胺肽稳定性的影响。随后,构建酒精损伤的GES-1细胞模型,通过检测细胞内部活性氧簇(reactive oxygen species,ROS)荧光强度、氧化损伤标志物、超氧化物歧化酶(superoxide dismutase,SOD)活力、谷胱甘肽(glutathione,GSH)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量,探究谷氨酰胺肽对氧化应激的抵抗能力和对细胞屏障的保护作用。结果表明,谷氨酰胺肽经消化后的分子质量比例变化均不超过1.5%,具备消化稳定性;谷氨酰胺肽和肽段pEQ、LQ均能减少活性氧的累积、减少丙二醛(malondialdehyde,MDA)含量,增强SOD活力,增加GSH和GSH-Px含量,且呈浓度依赖性,其中谷氨酰胺肽(800 μg/mL)减少活性氧累积和提高GSH、GSH-Px含量的效果最佳,LQ(60 μg/mL)降低MDA含量的效果最好,pEQ(60 μg/mL)提高SOD活力最佳,与模型组相比均有显著差异(P<0.05)。该研究证明谷氨酰胺肽能够显著提高GES-1细胞对酒精损伤的抵抗能力,为治疗或修复酒精性胃黏膜损伤以及保护胃黏膜屏障功能提供了支持。

关键词： 谷氨酰胺肽; 稳定性; GES-1细胞; 酒精损伤

本文引用格式

许润琦 , 王憬 , 孟德豪 , 张海欣 , 马永庆 , 毕园 , 徐国阳 , 陈亮 , 方磊 . 谷氨酰胺肽对乙醇诱导的胃黏膜损伤的保护作用研究[J]. 食品与发酵工业, 2025 , 51(20) : 239 -246 . DOI: 10.13995/j.cnki.11-1802/ts.040691

Abstract

This study investigated the protective effects of glutamine peptides (Gln-P) on ethanol-induced damage in GES-1 cells.Initially, to assess the stability of Gln-P throughout simulated gastrointestinal digestion, molecular weight distribution was utilized as a key evaluation criterion.Subsequently, an ethanol-damaged GES-1 cell model was established for evaluating resistance against oxidative stress of Gln-P and its effects on cellular barriers.The analysis encompassed key parameters such as the fluorescence intensity of intracellular reactive oxygen species (ROS), oxidative damage markers, superoxide dismutase (SOD) activity, glutathione (GSH) levels, and glutathione peroxidase (GSH-Px) levels.Results indicated that Gln-P maintained over 98.5% of its molecular weight after digestion, demonstrating robust digestive stability.Notably, Gln-P and its peptide fragments pEQ and LQ exhibited concentration-dependent reductions in ROS accumulation, malondialdehyde (MDA) content, and enhancements in SOD activity, GSH, and GSH-Px levels.Specifically, at a concentration of 800 μg/mL, Gln-P effectively mitigated ROS levels and enhanced GSH-Px activity.Additionally, LQ at 60 μg/mL demonstrated the most pronounced reduction in MDA, while pEQ at 60 μg/mL exhibited the highest increase in SOD activity.Notably, all these effects were statistically significant compared to the model group (P<0.05).This research highlights Gln-P’s considerable potential to strengthen GES-1 cells against ethanol damage.It also contributes to the advancement of therapeutic and reparative strategies aimed at addressing alcoholic gastric mucosal injury and protecting the gastric barrier.

Key words： glutamine peptide; stability; GES-1 cells; alcohol damage

参考文献

[1] CRUZAT V F, PANTALEÃO L C, DONATO J Jr, et al.Oral supplementations with free and dipeptide forms of L-glutamine in endotoxemic mice:Effects on muscle glutamine-glutathione axis and heat shock proteins[J].The Journal of Nutritional Biochemistry,2014,25(3):345-352.
[2] RUI C R, NEWSHOLME P, MARZUCA-NASSR G N, et al.Regulatory principles in metabolism-then and now[J].The Biochemical Journal,2016,473(13):1845-1857.
[3] 栗芳军.谷氨酰对胃癌术后肠道炎症恢复及免疫状况影响的研究[D].开封:河南大学,2013.
LI F J.A study on the effects of glutamine on intestinal inflammation recovery and immune status after gastric cancer surgery [D].Kaifeng:Henan University, 2013.
[4] YU H B, GAO Q F, DONG S L, et al.Regulation of dietary glutamine on the growth, intestinal function, immunity and antioxidant capacity of sea cucumber Apostichopus japonicus (Selenka)[J].Fish & Shellfish Immunology,2016,50:56-65.
[5] 陈思思.谷氨酰胺肽的制备及其性质的研究[D].无锡:江南大学,2014.
CHEN S S.Study on preparation and properties of glutamine peptide [D].Wuxi:Jiangnan University, 2014.
[6] 张丽.谷氨酰胺活性肽的制备及性质研究[D].合肥:合肥工业大学,2009.
ZHANG L.Preparation and properties of glutamine-derived bioactive peptides [D].Hefei:Hefei University of Technology, 2009.
[7] 郑燕斌,董娜,单安山.谷氨酰胺二肽生物学功能及在动物营养中的应用[J].动物营养学报,2011,23(8):1264-1268.
ZHENG Y B, DONG N, SHAN A S.Glutamine dipeptides:Biological function and its application in animal production[J].Chinese Journal of Animal Nutrition, 2011, 23(8):1264-1268.
[8] 任国谱,李玉英,谷文英.“条件必需氨基酸:Gln”生理功能的研究进展及应用现状[J].氨基酸和生物资源,2001,23(4):39-43.
REN G P, LI Y Y, GU W Y.Advancement in physiological function of gln:A conditionally essential amino acid and its use in nutrition[J] Amino Acids & Biotic Resources, 2001, 23(4):39-43.
[9] ABUMRAD N N, MORSE E L, LOCHS H, et al.Possible sources of glutamine for parenteral nutrition:Impact on glutamine metabolism[J].The American Journal of Physiology,1989,257(2 Pt1):E228-E234.
[10] SATO K, EGASHIRA Y, ONO S, et al.Identification of a hepatoprotective peptide in wheat gluten hydrolysate against D-galactosamine-induced acute hepatitis in rats[J].Journal of Agricultural and Food Chemistry, 2013, 61(26):6304-6310.
[11] SUZUKI Y, ASANO M, SATO K, et al.Wheat gluten hydrolysate alters the progress of hepatic pathology induced by prolonged carbon tetrachl oride administration in rat[J].Biomedical Research,2011,22(1):481-488.
[12] 林峰,马勇,徐亚光,等.基于分子质量分布的食源性低聚肽品质评价[J].食品与发酵工业,2008,34(9):128-131.
LIN F, MA Y, XU Y G, et al.Study on the quality evaluation of food-derived oligopeptides based on the molecular weight distribution[J].Food and Fermentation Industries, 2008, 34(9):128-131.
[13] 刘文颖, 谷瑞增, 林峰, 等.海洋骨胶原低聚肽钙配合物的稳定性[J].食品工业科技, 2015, 36(4):111-115.
LIU W Y, GU R Z, LIN F, et al.Stability of calcium-chelating marine bone collagen oligopeptides[J].Science and Technology of Food Industry, 2015, 36(4):111-115.
[14] 赵荣, 张英雪, 单春乔, 等.酶解对豆粕营养成分及体外消化率的影响[J].粮食与饲料工业, 2019(1):41-43.
ZHAO R, ZHANG Y X, SHAN C Q, et al.Effects of enzymatic hydrolysis on nutritional components and digestibility of soybean meal[J].Cereal & Feed Industry, 2019(1):41-43.
[15] 冯晓文, 赵晓涵, 潘骁琦, 等.体外模拟消化对海洋鱼骨胶原低聚肽结构和抗氧化活性的影响[J].食品与发酵工业, 2022,48(5):173-179.
FENG X W, ZHAO X H, PAN X Q, et al.The effect of simulated digestion in vitro on structure and antioxidant activity of marine fish bone collagen oligopeptides[J].Food and Fermentation Industries, 2022, 48(5):173-179.
[16] 李明亮,许锦珍,冯志远,等.二肽AQ、SQ和IQ增强GES-1细胞对酒精损伤的抵抗能力[J].食品与发酵工业,2023,49(6):143-148.
LI M L, XU J Z, FENG Z Y, et al.Enhancement of dipeptides AQ, SQ, and IQ on the resistance of GES-1 cells to alcohol damage[J].Food and Fermentation Industries, 2023, 49(6):143-148.
[17] 方磊,李诒光,陈亮,等.小麦低聚肽营养成分和特征功能肽段研究[J].食品与发酵工业,2023,49(11):178-182.
FANG L, LI Y G, CHEN L, et al.Study on nutritional components and characteristic functional peptides of wheat oligopeptides[J].Food and Fermentation Industries, 2023, 49(11):178-182.
[18] 秦书敏.黄芪甲苷对乙醇诱导的大鼠胃黏膜损伤的保护作用及机制研究[D].广州:广州中医药大学,2017.
QIN S M.Protective effects and mechanisms of astragaloside Ⅳ on ethanol-induced gastric mucosal injury in rats [D].Guangzhou:Guangzhou University of Chinese Medicine, 2017.
[19] 凌空,张铭晧,高丽辉,等.体外模拟消化对小麦低聚肽抗氧化活性影响[J].食品工业,2021,42(4):82-86.
LING K, ZHANG M H, GAO L H, et al.Effect on the antioxidant activity of wheat oligopeptides after in vitro digestion[J].The Food Industry, 2021, 42(4):82-86.
[20] 朱艳,李明亮,张瑞雪,等.自主研发复合低聚肽对乙醇损伤的GES-1细胞修复作用的研究[J].食品科技,2020,45(05):24-30.
ZHU Y, LI M L, ZHANG R X, et al.Study on the cytoprotection of combined oligopeptide on ethanol-induced GES-1 cells damage[J].Food Science and Technology, 2020, 45(5):24-30.
[21] FISKUM G, ROSENTHAL R E, VERECZKI V, et al.Protection against ischemic brain injury by inhibition of mitochondrial oxidative stress.[J].Journal of Bioenergetics and Biomembranes,2004,36(4):347-352.
[22] 方磊,郭颖,朱艳,等.紫苏籽肽及其协同硒解酒护肝作用研究[J].食品科技,2020,45(7):216-222.
FANG L, GUO Y, ZHU Y, et al.Study on Perilla seed peptide and its synergistic effect on selenium-relieving wine[J].Food Science and Technology, 2020, 45(7):216-222.
[23] 朱向东,王燕,何兰娟,等.四神丸对溃疡性结肠炎模型大鼠结肠组织Toll样受体2、4表达的影响[J].中国中医药信息杂志,2016,23(10):67-71.
ZHU X D, WANG Y, HE L J, et al.Effects of Sishen pills on expressions of TLR-2 and TLR-4 of colonic tissue in rats with ulcerative colitis[J].Chinese Journal of Information on Traditional Chinese Medicine, 2016, 23(10):67-71.
[24] 阳丹,蔡舒,彭成海,等.老香黄多糖的分离纯化及其体外胃黏膜保护活性研究[J].食品工业科技,2023,44(19):440-448.
YANG D, CAI S, PENG C H, et al.Studies on isolation, purification and gastric mucosal protective activity in vitro of Laoxianghuang polysaccharide[J].Science and Technology of Food Industry, 2023, 44(19):440-448.
[25] YONG Y, ZHANG C F, GU Z J, et al.Polyoxometalate-based radiosensitization platform for treating hypoxic tumors by attenuating radioresistance and enhancing radiation response.[J].ACS Nano,2017,11(7):7164-7176.
[26] 孙跃博,罗瑞卿,陈洁,等.中药对围产期奶牛血液免疫及抗氧化活性指标的影响[J].西北农业学报,2015,24(11):18-23.
SUN Y B, LUO R Q, CHEN J, et al.Effects of traditional Chinese medicine on the hematogenic immunity and antioxidant activity of perinatal cows[J].Acta Agriculturae Boreali-Occidentalis Sinica, 2015, 24(11):18-23.
[27] EJIMA A, NAKAMURA M, SUZUKI Y A, et al.Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates[J].Journal of Food Bioactives,2018:104-111.
[28] 李剑锋. 富含支链氨基酸的氨基酸配方对胃肠外科手术创伤患者的营养支持效果研究. 名医, 2018(9):236.
LI J F. Effect of the formula of amino acid enriched branch chain amino acid on nutritional support in postoperative traumatic patients. Renowned Doctor, 2018(9):236.
[29] 蒋竹青.玉米高F值寡肽的制备及生理功能研究[D].济南:济南大学,2015.
JIANG Z Q.Preparation and physiological function of high F-value oligopeptides from corn [D].Jinan:University of Jinan, 2015.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司