便秘作为一种常见的胃肠道疾病,严重影响患者的生活质量,并干扰其正常的日常活动。研究发现,便秘与肠道菌群失调有关。前期小鼠实验发现,鼠李糖乳酪杆菌CCFM1068具有缓解便秘的作用。基于此,该文旨在通过人群实验评估鼠李糖乳酪杆菌CCFM1068对便秘的影响及其在改善便秘患者肠道菌群方面的作用。该研究在无锡市第二人民医院招募25名便秘患者,经过一周访问期,符合条件的患者进行为期4周的益生菌干预。每位患者在干预前后各采集一次粪便,用来分析肠道菌群的变化。同时,评估每周自发排便、布里斯托尔粪便量表、便秘患者症状和生活质量。结果显示,鼠李糖乳酪杆菌CCFM1068干预4周后便秘患者的排便次数、粪便分型和生活质量显著改善。其次,患者的肠道菌群整体结构发生显著变化。门水平上,显著上调了Firmicutes的丰度,下调了Bacteroidetes的丰度。属水平上,鼠李糖乳酪杆菌CCFM1068通过下调Bacteroides、Phocaeicola、Alistipes、Parabacteroides、Odoribacter和Butyricimonas的丰度,上调Dorea、Clostridium、Anaerobutyricum、Raoultella、Adlercreutzia、Megamonas、Mediterraneibacter、Streptococcus和Escherichia的丰度,改善粪便稠度,缓解便秘。这些发现为后期应用益生菌缓解便秘提供了理论基础。
Constipation, a common gastrointestinal disorder, significantly impacts the life quality of patients and disrupts their daily activities.Studies have shown that constipation is associated with gut microbiota dysbiosis.Previous animal studies have demonstrated that Lacticaseibacillus rhamnosus CCFM1068 alleviates constipation.Building on this, this study aimed to evaluate the effect of L.rhamnosus CCFM1068 on constipation and its role in improving the gut microbiota in constipated individuals through clinical trials.In this study, 25 patients with constipation were recruited from Wuxi Second People’s Hospital.After a one-week screening period, eligible patients underwent a four-week probiotic intervention.Fecal samples were collected from each patient before and after the intervention to analyze changes in gut microbiota.Concurrently, spontaneous bowel movements (SBM), the bristol stool form scale (BSFS), the patient assessment of constipation symptoms (PAC-SYM), and the quality of life (PAC-QOL) were assessed weekly.Results indicated that after four weeks of L.rhamnosus CCFM1068 intervention, the frequency of defecation, stool consistency, and life quality of patients were significantly improved.Furthermore, the overall composition of the gut microbiota in patients underwent significant changes.At the phylum level, Firmicutes abundance was significantly increased, while Bacteroidetes abundance was decreased.At the genus level, L.rhamnosus CCFM1068 improved stool consistency and alleviated constipation by downregulating the abundance of Bacteroides, Phocaeicola, Alistipes, Parabacteroides, Odoribacter, and Butyricimonas, while upregulating that of Dorea, Clostridium, Anaerobutyricum, Raoultella, Adlercreutzia, Megamonas, Mediterraneibacter, Streptococcus, and Escherichia.These findings provide a theoretical foundation for the future application of probiotics in alleviating constipation.
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