甜酒糟对高温大曲糖化能力的强化作用及其在末轮次酱酒发酵中的应用

  • 周慧玲 ,
  • 秦洋 ,
  • 赵千慧
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  • 1(邵阳学院 食品与化学工程学院,湖南 邵阳,422000)
    2(邵阳学院,生态酿酒新技术与应用湖南省高校重点实验室,湖南 邵阳,422000)
第一作者:硕士研究生(秦洋讲师为通信作者,E-mail:466031343@ qq.com)

收稿日期: 2025-03-05

  修回日期: 2025-05-27

  网络出版日期: 2025-11-03

基金资助

湖南省研究生科研创新立项不资助项目(LXBZZ2024397);湖南省普通高等学校科技创新团队支持资助项目(湘教通[2023]233号);中央引导地方发展资金项目(2024ZYC029)

Reinforcing effect of sweet wine lees on saccharification ability of high-temperature Daqu and its application in final round of Jiangxiangxing Baijiu fermentation

  • ZHOU Huiling ,
  • QIN Yang ,
  • ZHAO Qianhui
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  • 1(College of Food and Chemical Engineering, Shaoyang University, Shaoyang 422000, China)
    2(Hunan Provincial Higher Education Key Laboratory of New Technology and Application for Ecological Baijiu Production, Shaoyang University, Shaoyang 422000, China)

Received date: 2025-03-05

  Revised date: 2025-05-27

  Online published: 2025-11-03

摘要

酱香型白酒历史悠久,具有多轮次发酵和蒸煮特点。七轮次发酵后,末轮次酒醅残余淀粉利用率低,糖化效率低。大小曲联用技术可提高白酒的糖化能力,甜酒糟可视为甜酒曲中糖化微生物的活化以及扩培产物,其中富含的米根霉等微生物被证实有分泌淀粉酶的能力,但目前甜酒糟还未被应用于末轮次酱酒糖化领域。该文旨在探讨甜酒糟和甜酒曲对高温大曲糖化能力的强化作用从而提高末轮次酱酒的糖化效率和粮食利用率。该研究优选合适的甜酒糟和甜酒曲添加量,并将其应用于末轮次酱酒醅发酵,对酿造后的酒醅淀粉进行直链淀粉含量、X射线衍射、扫描电子显微镜、凝胶渗透色谱检测,揭示甜酒糟对酒醅淀粉的促进作用。结果表明,7 g/50 g甜酒糟和3.5 g/50 g甜酒曲为最佳添加量。模拟末轮次酱酒发酵实验显示,甜酒糟显著提升糖化效率,残余淀粉消耗量较空白组提高15.38%,还原糖生成量增加12%。甜酒糟强化组的小分子质量淀粉含量增加,淀粉结晶度降低、淀粉颗粒表面裂痕和孔洞增多。从分子水平说明甜酒糟对末轮次酱酒醅糖化过程具有促进作用。而甜酒曲强化组作用较弱,残余淀粉消耗量仅提高2.56%。因此,在传统大曲中添加甜酒糟可有效提高末轮次酱酒醅的糖化效率,为淀粉资源的最大化利用提供理论依据。

本文引用格式

周慧玲 , 秦洋 , 赵千慧 . 甜酒糟对高温大曲糖化能力的强化作用及其在末轮次酱酒发酵中的应用[J]. 食品与发酵工业, 2025 , 51(19) : 48 -55 . DOI: 10.13995/j.cnki.11-1802/ts.042616

Abstract

The Jiangxiangxing Baijiu has a long history and is characterized by multiple cycles of fermentation.The final round of Jiangxiangxing Baijiu is limited by low starch utilization and inefficient saccharification.In the past, co-fermentation with Daqu and Xiaoqu could enhance the saccharification capacity of Baijiu, while sweet wine lees can be regarded as an activated state of sweet wine koji.However, sweet wine lees have not been applied in final round of Jiangxiangxing Baijiu.This study aims to improve the saccharification capacity of traditional high-temperature Daqu by sweet wine lees to improve the grain utilization.The appropriate amount of sweet wine lees or sweet wine koji were optimized and then applying them into the final round of Jiangxiangxing Baijiu fermentation.The amylose content, X-ray diffraction, scanning electron microscopy, and gel permeation chromatography were detected to explain the saccharification-enhancing mechanism.Results showed that the sweet wine lees with an addition of 7 g/50 g and the sweet wine koji with an addition of 3.5 g/50 g were optimized as the ideal groups by single-factor experiments.In the final round of Jiangxiangxing Baijiu fermentation, compared with the control group, the sweet wine lees group consumed 15.38% more starch and increased the reducing sugar content by 12%, while the sweet wine koji group only consumed 2.56% more starch compared to the control group, indicating that the effect of sweet wine koji was not significant.In a series of starch experiments, the sweet wine lees group had lower starch crystallinity, smaller molecular weights, and more fragmented particle morphology.These phenomena indicated the mechanism of saccharification ability enhancement by sweet wine lees.This paper reflects that sweet wine lees can significantly improve the saccharification efficiency of fermented sorghum, providing academic support for the utilization of starch resources.

参考文献

[1] LIU H L, SUN B G.Effect of fermentation processing on the flavor of Baijiu[J].Journal of Agricultural and Food Chemistry, 2018, 66(22):5425-5432.
[2] 戴奕杰, 李宗军, 田志强.酱香型白酒酒醅酶活性和轮次酒理化指标分析[J].中国酿造, 2019, 38(2):31-36.
DAI Y J, LI Z J, TIAN Z Q.Enzyme activity of fermented grains of sauce-flavor Baijiu and analysis of physical and chemical index of rounds Baijiu[J].China Brewing, 2019, 38(2):31-36.
[3] 付庆凯, 陈帆, 孟廷淋, 等.酱香型白酒不同轮次发酵酒醅差异性研究[J/OL].食品与发酵工业, 2025:1-11.(2025-01-26).DOI:10.13995/j.cnki.11-1802/ts.040940.
FU Q K, CHEN F, MENG T L, et al.Study on differences in fermented grains across different fermentation rounds of Jiangxiangxing Baijiu[J/OL].Food and Fermentation Industries, 2025:1-11.(2025-01-26).DOI:10.13995/j.cnki.11-1802/ts.040940.
[4] 张兆丰, 万勇, 余有贵, 等.浓香型白酒生产中小曲堆积糖化的工艺研究[J].酿酒科技, 2022(2):79-86;98.
ZHANG Z F, WAN Y, YU Y G, et al.Stacking saccharification with xiaoqu in the production of Nongxiang Baijiu[J].Liquor-Making Science & Technology, 2022(2):79-86;98.
[5] 徐国俊, 张玉, 蔡雄, 等.大小曲混合发酵苦荞酒工艺研究及风味成分分析[J].食品工业科技, 2015, 36(6):225-229;244.
XU G J, ZHANG Y, CAI X, et al.Mixed Tartary buckwheat wine fermentation process by Daqu and Xiaoqu and analysis of flavoring component[J].Science and Technology of Food Industry, 2015, 36(6):225-229;244.
[6] 孙祥祥, 石飞, 刘长虹, 等.米酒作为馒头传统发酵剂的生产工艺研究[J].中国酿造, 2019, 38(2):144-147.
SUN X X, SHI F, LIU C H, et al.Optimization of production process of rice wine used for steamed bread traditional starter[J].China Brewing, 2019, 38(2):144-147.
[7] 梁雪梅. 甜酒曲中酵母菌筛选及复配发酵提升醪糟品质研究[D].成都:西华大学, 2023.
LIANG X M.Screening the yeast from sweet wine koji and improving the quality of Laozao by the cooperative fermentation[D].Chengdu:Xihua University, 2023.
[8] 康宪, 李良红, 林捷.传统桂林甜酒曲中酵母分离鉴定及发酵性能研究[J].食品工业, 2017, 38(1):126-129.
KANG X, LI L H, LIN J.Isolation, identification and research of fermentation characteristics of yeast from traditional Guilin rice wine koji[J].The Food Industry, 2017, 38(1):126-129.
[9] LIU F, HE Y, WANG L, et al.Feasibility of the use of visible and near infrared spectroscopy to assess soluble solids content and pH of rice wines[J].Journal of Food Engineering, 2007, 83(3):430-435.
[10] LEE M E, SHIN H Y, BHARDWAJ N, et al.Effective bioconversion of fungal-spoiled starchy food waste into fermentable sugars using fungi-degrading, artificial amylosomes[J].Bioresource Technology, 2023, 388:129760.
[11] QI W J, KONG S Y, LI X Q, et al.Insight into characteristics in rice starch under heat- moisture treatment:Focus on the structure of amylose/amylopectin[J].Food Chemistry:X, 2024, 24:101942.
[12] 闫华文. 甜酒曲中真菌多样性的研究及甜酒酿营养成分分析[D].曲阜:曲阜师范大学, 2015.
YAN H W.Fungal diversity in sweet wine koji and nutritional composition analysis of fermented sweet rice[D].Qufu:Qufu Normal University, 2015.
[13] 何璇, 高银涛, 余博文, 等.酱香型白酒发酵过程全周期跟踪分析[J].食品与发酵工业, 2022, 48(10):199-205.
HE X, GAO Y T, YU B W, et al.Tracking and analysis of the whole fermentation process of Maotai-flavor Baijiu[J].Food and Fermentation Industries, 2022, 48(10):199-205.
[14] 杨凤英, 秦洋.冠突散囊菌对酒曲糖化能力的影响及其机制研究[J].食品与发酵工业, 2023, 49(5):81-87.
YANG F Y, QIN Y.Impact and mechanism of Eurotium critatum on the saccharification ability of Jiuqu[J].Food and Fermentation Industries, 2023, 49(5):81-87.
[15] 赵千慧, 周慧玲, 秦洋.冠突散囊菌对高粱酒酿造过程糖化效率的促进作用与原理[J].食品与发酵工业, 2024, 50(15):72-78.
ZHAO Q H, ZHOU H L, QIN Y.Study on saccharification promoting of the sorghum Baijiu by Eurotium cristatum and its mechanism[J].Food and Fermentation Industries, 2024, 50(15):72-78.
[16] SUN X X, SUN Z Z, SALEH A S M, et al.Understanding the granule, growth ring, blocklets, crystalline and molecular structure of normal and waxy wheat A- and B- starch granules[J].Food Hydrocolloids, 2021, 121:107034.
[17] LIAN X J, CHENG K L, WANG D L, et al.Analysis of crystals of retrograded starch with sharp X-ray diffraction peaks made by recrystallization of amylose and amylopectin[J].International Journal of Food Properties, 2017, 20(sup3):S3224-S3236.
[18] ZHANG S J, WANG Z, ZHOU X P, et al.Insights into the regulation mechanisms of dual hydrothermal treatment on the structure and digestive characteristics of A- and B-type wheat starch granules[J].Food Research International, 2025, 200:115448.
[19] 邹玉婷, 贺紫涵, 许碧涛, 等.酒曲中产酶微生物的筛选及其对发酵米酒品质的影响[J].食品科学, 2024, 45(10):108-117.
ZOU Y T, HE Z H, XU B T, et al.Selection of enzyme-producing microorganisms from jiuqu and their influence on the quality of fermented rice wine[J].Food Science, 2024, 45(10):108-117.
[20] 任丽霞. 甜酒曲微生物群落结构分析及八二曲的改良研究[D].上海:上海应用技术大学, 2020.
REN L X.Microbial community structure analysis of sweet wine koji and improvement of Baer Qu[D].Shanghai:Shanghai Institute of Technology, 2020.
[21] 何小容, 张庆, 唐家环, 等.浓香型白酒大曲发酵过程中主要微生物群系及其作用研究进展[J].中国酿造, 2024, 43(8):1-6.
HE X R, ZHANG Q, TANG J H, et al.Research progress of main microflora and their roles during strong-flavor Baijiu Daqu fermentation[J].China Brewing, 2024, 43(8):1-6.
[22] 周金虎, 陈茂彬, 毛志海, 等.甜酒曲中一株高产淀粉酶根霉的筛选与鉴定[J].酿酒科技, 2019(1):37-40.
ZHOU J H, CHEN M B, MAO Z H, et al.Screening and identification of a Rhizopus strain with high-yield of amylase from Jiuniang starter[J].Liquor-Making Science & Technology, 2019(1):37-40.
[23] 韩文芳, 林亲录, 赵思明, 等.直链淀粉和支链淀粉分子结构研究进展[J].食品科学, 2020, 41(13):267-275.
HAN W F, LIN Q L, ZHAO S M, et al.Recent advances in molecular structures of amylose and amylopectin[J].Food Science, 2020, 41(13):267-275.
[24] CHEN X Y, ZHU L, ZHANG H, et al.Effects of composition, starch structural orders, and kernel structure on starch in vitro digestion of highland barley[J].Carbohydrate Polymers, 2023, 301:120274.
[25] YANG Q H, ZHANG W L, LUO Y, et al.Comparison of structural and physicochemical properties of starches from five coarse grains[J].Food Chemistry, 2019, 288:283-290.
[26] 庞臻, 马凤丽, 程平言, 等.双波长比色法测定高粱中支链淀粉占总淀粉比例[J].食品工业, 2024, 45(7):316-322.
PANG Z, MA F L, CHENG P Y, et al.Determination of the proportion of amylopectin to total starch in sorghum by dual-wavelength method[J].The Food Industry, 2024, 45(7):316-322.
[27] ASARE E K, JAISWAL S, MALEY J, et al.Barley grain constituents, starch composition, and structure affect starch in vitro enzymatic hydrolysis[J].Journal of Agricultural and Food Chemistry, 2011, 59(9):4743-4754.
[28] 史盈盈, 谈宇婷, 张倩, 等.小麦淀粉结构、特性及改性和应用研究进展[J].食品科技, 2025, 50(1):240-247.
SHI Y Y, TAN Y T, ZHANG Q, et al.Research progress on structure, characterization, modification and applications of wheat starch[J].Food Science and Technology, 2025, 50(1):240-247.
[29] SUN X X, SUN Z Z, SALEH A S M, et al.Understanding the granule, growth ring, blocklets, crystalline and molecular structure of normal and waxy wheat A- and B- starch granules[J].Food Hydrocolloids, 2021, 121:107034.
[30] WANG Y J, XIE Z F, WU Q, et al.Preparation and characterization of carboxymethyl starch from cadmium-contaminated rice[J].Food Chemistry, 2020, 308:125674.
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