食品与发酵工业 >

2025 , Vol. 51 >Issue 19: 118 - 125

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042954

骆驼乳和牛乳外泌体miRNAs表达谱的测序与分析

  • 许建春 ,
  • 王建程 ,
  • 方志峰 ,
  • 樊哲新 ,
  • 李宝坤
展开
  • 1(农业农村部特色农产品加工与质量安全控制重点实验室(部省共建),石河子大学 食品学院,新疆 石河子,832000)
    2(兵团食品营养与安全控制重点实验室,石河子大学 食品学院,新疆 石河子,832000)
    3(新疆特色果蔬贮藏加工教育部工程研究中心,石河子大学 食品学院,新疆 石河子,832000)
第一作者:硕士研究生(樊哲新副教授为通信作者,E-mail:zhexin_fan@shzu.edu.cn)

收稿日期: 2025-04-08

  修回日期: 2025-05-08

  网络出版日期: 2025-11-03

基金资助

国家自然科学基金项目(32260594);石河子大学高层次人才科研启动项目(2022ZK012);兵团财政科技计划项目(2023ZD087)

收起

Sequencing and analysis of miRNAs expression profiles in camel milk and bovine exosomes

  • XU Jianchun ,
  • WANG Jiancheng ,
  • FANG Zhifeng ,
  • FAN Zhexin ,
  • LI Baokun
Expand
  • 1(Key Laboratory of Characteristics Agricultural Product Processing and Quality Control (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, School of Food Science and Technology, Shihezi University, Shihezi 832000, China)
    2(Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi 832000, China)
    3(Engineering Research Center of Storage and Processing of Xinjiang Characteristic Fruits and Vegetables Ministry of Education, School of Food Science and Technology, Shihezi University, Shihezi 832000, China)

Received date: 2025-04-08

  Revised date: 2025-05-08

  Online published: 2025-11-03

Fold

摘要

采用超高速离心法提取骆驼乳和牛乳外泌体。利用Illumina 6000技术对骆驼乳和牛乳外泌体中非编码小RNA(small RNA, sRNA)进行测序,将纯净sRNA与数据库比对后筛选出微小RNA(micro RNA,miRNAs)进行对比分析和生物学信息分析。在骆驼乳外泌体中鉴定出6 985种已知miRNAs和86种新miRNAs;牛乳外泌体中鉴定出8 675种已知miRNAs和229种新miRNAs,其中let-7a、let-7a-5p、let-7b、let-7b-5p、let-7、miRNA-148a-3p和miR-30a-5p等miRNAs在骆驼乳和牛乳中表达量较高。基因本体论富集分析发现牛乳外泌体miRNAs靶基因在细胞过程和结合功能富集数量最多;骆驼乳外泌体miRNAs还在催化活性上显示出巨大潜力,对细胞新陈代谢有一定促进作用。京都基因与基因组百科全书富集分析发现,骆驼乳和牛乳外泌体miRNAs靶基因分子均在糖尿病并发症RNA完整值(RNA integrity number,RIN)信号通路、胰岛素抵抗通路和癌症中的通路显著富集。结果提示骆驼乳和牛乳外泌体来源的miRNAs,如miR-92a、miR-10240-5p、miR-421-3p和miR-185-5p等可以作为糖尿病的潜在治疗靶点。上述miRNAs对靶基因的调控可能与乳的有益作用密切相关。

关键词： 骆驼乳外泌体; 牛乳外泌体; Illumina测序; miRNAs; 糖尿病并发症AGE-RAGE信号通路; 胰岛素抵抗

本文引用格式

许建春 , 王建程 , 方志峰 , 樊哲新 , 李宝坤 . 骆驼乳和牛乳外泌体miRNAs表达谱的测序与分析[J]. 食品与发酵工业, 2025 , 51(19) : 118 -125 . DOI: 10.13995/j.cnki.11-1802/ts.042954

Abstract

Ultracentrifugation was used to extract exosomes from camel milk and bovine milk.Non-coding small RNAs (sRNA) were sequenced and identified by Illumina 6000 sequencing technology.Following the alignment of clean sRNA with relevant databases, miRNAs from camel milk and bovine milk exosomes were identified for comparative and biological information analysis.A total of 6 985 known miRNAs and 86 novel miRNAs were identified in camel milk exosomes.A total of 8 675 known miRNAs and 229 novel miRNAs sequences were identified in bovine milk exosomes.Among the known miRNAs, let-7a, let-7a-5p, let-7b, let-7b-5p, let-7, miRNA-148a-3p, and miR-30a-5p had the high expression levels.The results of gene ontology enrichment analysis indicated that the target genes of miRNAs from bovine milk exosomes were significantly enriched in cellular processes and binding functions.Furthermore, target genes of miRNAs derived from camel milk exosomes demonstrated considerable potential in catalytic activity, contributing to cellular metabolism.According to the results of the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, the target genes of miRNAs in both camel and bovine milk exosomes were involved in the AGE-RAGE signalling pathway in diabetic complications, the insulin resistance pathway, and cancer pathways.These findings suggested that miRNAs, such as miR-92a, miR-10240-5p, miR-421-3p, and miR-185-5p, serve as potential therapeutic targets for diabetes.The regulation of target genes by the above-mentioned miRNAs may be closely related to the beneficial effects of milk.

Key words： camel milk exosomes; bovine milk exosomes; Illumina sequencing; miRNAs; AGE-RAGE signalling pathway in diabetic complications; insulin resistance

参考文献

[1] PEREIRA P C.Milk nutritional composition and its role in human health[J].Nutrition, 2014, 30(6):619-627.
[2] 黄新红, 郭文振, 王旭涛, 等. 骆驼乳营养成分变化规律及产业化应用研究[J]. 乳品与人类, 2024, (4): 26-31.
HUANG X H, GUO W Z, WANG X T, et al.Nutritional Composition of Camel Milk: Changing Laws and Industrialization Applications, 2024, (4): 26-31.
[3] YADAV A K, KUMAR R, PRIYADARSHINI L, et al.Composition and medicinal properties of camel milk:A review[J].Asian Journal of Dairy and Food Research, 2015, 34(2):83.
[4] ARAB H H, SALAMA S A, EID A H, et al.Camel’s milk ameliorates TNBS-induced colitis in rats via downregulation of inflammatory cytokines and oxidative stress[J].Food and Chemical Toxicology, 2014, 69:294-302.
[5] CHEN Y Z, LI C, GU J, et al.Anti-oxidative and immuno-protective effect of camel milk on radiation-induced intestinal injury in C57BL/6J mice[J].Dose-response, 2021, 19(1):15593258211003798.
[6] KORISH A A.The antidiabetic action of camel milk in experimental type 2 diabetes mellitus:An overview on the changes in incretin hormones, insulin resistance, and inflammatory cytokines[J].Hormon- und Stoffwechselforschung, 2014, 46(6):404-411.
[7] KALLURI R, LEBLEU V S.The biology, function, and biomedical applications of exosomes[J].Science, 2020, 367(6478):EAAU6977.
[8] GOLAN-GERSTL R, REIF S.Extracellular vesicles in human milk[J].Current Opinion in Clinical Nutrition & Metabolic Care, 2022, 25(3):209-215.
[9] REIF S, ELBAUM-SHIFF Y, KOROUKHOV N, et al.Cow and human milk-derived exosomes ameliorate colitis in DSS murine model[J].Nutrients, 2020, 12(9):2589.
[10] IBRAHIM H M, MOHAMMED-GEBA K, TAWFIC A A, et al.Camel milk exosomes modulate cyclophosphamide-induced oxidative stress and immuno-toxicity in rats[J].Food & Function, 2019, 10(11):7523-7532.
[11] ZHANG J, LI S, LI L, et al.Exosome and exosomal microRNA:Trafficking, sorting, and function[J].Genomics, Proteomics & Bioinformatics, 2015, 13(1):17-24.
[12] PENG H, JI W H, ZHAO R C, et al.Exosome:A significant nano-scale drug delivery carrier[J].Journal of Materials Chemistry B, 2020, 8(34):7591-7608.
[13] BARTEL D P.MicroRNAs:genomics, biogenesis, mechanism, and function[J].Cell, 2004, 116(2):281-297.
[14] LI D, YAO X L, YUE J X, et al.Advances in bioactivity of microRNAs of plant-derived exosome-like nanoparticles and milk-derived extracellular vesicles[J].Journal of Agricultural and Food Chemistry, 2022, 70(21):6285-6299.
[15] 罗雨佳, 黄子彧, 林莹莹, 等.不同泌乳期人乳外泌体miRNA表达谱的研究[J].中国食品学报, 2022, 22(11):335-342.
LUO Y J, HUANG Z Y, LIN Y Y, et al.Exploration of exosomal miRNA expression profiles in human milk during the different lactation period[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(11):335-342.
[16] 柴玉霞, 王新宇, 岳喜庆, 等.驴乳外泌体中miRNA的测序与分析[J].食品科学, 2022, 43(14):151-157.
CHAI Y X, WANG X Y, YUE X Q, et al.Sequencing and analysis of microRNAs in donkey milk exosomes[J].Food Science, 2022, 43(14):151-157.
[17] 鲁曦, 任珂.山羊乳及绵羊乳外泌体miRNAs表达谱的分析与差异比较[J].食品与发酵工业, 2023, 49(23):134-140.
LU X, REN K.Analysis and comparison of miRNAs expression profiles of goat milk and sheep milk exosomes[J].Food and Fermentation Industries, 2023, 49(23):134-140.
[18] 商静雯, 柴玉霞, 曹雪妍, 等.牛乳外泌体中miRNA的测序与分析[J].食品科学, 2023, 44(24):105-111.
SHANG J W, CHAI Y X, CAO X Y, et al.Sequencing and analysis of microRNAs in bovine milk exosomes[J].Food Science, 2023, 44(24):105-111.
[19] SHANG J W, NING J T, BAI X, et al.Identification and analysis of miRNAs expression profiles in human, bovine, and donkey milk exosomes[J].International Journal of Biological Macromolecules, 2023, 252:126321.
[20] CENDRON F, ROSANI U, FRANZOI M, et al.Analysis of miRNAs in milk of four livestock species[J].BMC Genomics, 2024, 25(1):859.
[21] ALSAWEED M, LAI C T, HARTMANN P E, et al.Human milk cells contain numerous miRNAs that may change with milk removal and regulate multiple physiological processes[J].International Journal of Molecular Sciences, 2016, 17(6):956.
[22] SHAH K B, FIELDS D A, PEZANT N P, et al.Gestational diabetes mellitus is associated with altered abundance of exosomal microRNAs in human milk[J].Clinical Therapeutics, 2022, 44(2):172-185.e1.
[23] ZHANG C, ZHENG J, HAN X T, et al.Bovine colostrum miR-30a-5p targets the NF-κB signaling pathway to alleviate inflammation in intestinal epithelial cells[J].Journal of Agricultural and Food Chemistry, 2024.
[24] WALKE P B, BANSODE S B, MORE N P, et al.Molecular investigation of glycated insulin-induced insulin resistance via insulin signaling and AGE-RAGE axis[J].Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2021, 1867(2):166029.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司