食品与发酵工业 >

2025 , Vol. 51 >Issue 19: 238 - 247

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042967

基于纳米碳酸钙的牡蛎肽递送体系构建与结构表征及其消化特性研究

  • 王婧 ,
  • 张萌萌 ,
  • 张卓铅 ,
  • 曹文红 ,
  • 谭明堂 ,
  • 朱国萍 ,
  • 高加龙 ,
  • 林海生 ,
  • 郑惠娜 ,
  • 陈忠琴
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  • 1(广东海洋大学 食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,广东省水产预制食品加工与品质控制工程技术研究中心,广东 湛江,524088)
    2(广东海洋大学 深圳研究院,广东 深圳,518120)
第一作者:硕士研究生(陈忠琴副教授为通信作者,E-mail:chenzhongqin@gdou.edu.cn)

收稿日期: 2025-04-09

  修回日期: 2025-05-30

  网络出版日期: 2025-11-03

基金资助

湛江市海洋青年人才创新项目(2021E05017);广东海洋大学科研启动费资助项目(060302042007);国家自然科学基金项目(32201971);广东省基础与应用基础研究基金项目(2024A1515011763);广东省现代农业产业技术体系贝藻创新团队项目(2024CXTD23)

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Construction, structural characterization and digestion characteristics of oyster peptides delivery system based on nano-calcium carbonate

  • WANG Jing ,
  • ZHANG Mengmeng ,
  • ZHANG Zhuoqian ,
  • CAO Wenhong ,
  • TAN Mingtang ,
  • ZHU Guoping ,
  • GAO Jialong ,
  • LIN Haisheng ,
  • ZHENG Huina ,
  • CHEN Zhongqin
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  • 1(College of Food Science and Technology, Guangdong Ocean University, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China)
    2(Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China)

Received date: 2025-04-09

  Revised date: 2025-05-30

  Online published: 2025-11-03

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摘要

牡蛎肽具有抗氧化、降血糖、降血脂等多种生物活性,但其在胃肠道环境中稳定性差的问题严重限制了其活性发挥。为提高牡蛎肽的稳定性及减少牡蛎壳资源的浪费,该研究首先以牡蛎壳为原料制备了纳米碳酸钙(nano-calcium carbonate,CaCO3 NPs),然后采用海藻酸钠(sodium alginate,ALG)对其进行修饰,并优化修饰工艺;最后将牡蛎肽负载到ALG-CaCO3 NPs上,对其进行聚乙二醇修饰,构建了牡蛎肽-CaCO3 NPs纳米体系,并进一步探究其结构稳定性、体外释放及消化特性。正交试验结果表明,CaCO3 NPs的最佳修饰工艺参数为:ALG质量浓度5 mg/mL、温度35 ℃、反应时间2 h。结构表征结果表明,牡蛎肽-CaCO3 NPs纳米体系平均粒径为(288.7±7.6) nm,电位为(-30.5±0.8) mV,其包封率和装载量分别为(35.69±2.22)%和(19.71±1.60)%。傅里叶红外光谱表明ALG通过羧基与Ca2+交联形成“蛋盒”结构对CaCO3 NPs进行修饰,而牡蛎肽是通过静电作用负载于纳米体系内部。体外释放实验结果显示,牡蛎肽-CaCO3 NPs纳米体系在模拟胃环境(pH 2.0)中36 h累积释放率仅为(40.31±0.63)%,而在肠环境(pH 6.8)中释放率显著提升至(93.12±3.76)%,表明其具有靶向肠道缓释特性。消化稳定性实验表明,牡蛎肽-CaCO3 NPs纳米体系可显著提高牡蛎肽的胰脂肪酶抑制活性,胃消化后抑制率从(23.66±0.93)%提升至(54.73±1.37)%,胃肠消化后抑制率从(4.75±0.38)%提升至(24.67±1.31)%。研究结果为功能性肽类的稳定化递送系统设计提供了理论支持,同时为牡蛎壳废弃物的高值化利用提供了新途径。

关键词： 牡蛎肽; 纳米碳酸钙; 海藻酸钠修饰; 纳米递送系统; 消化特性

本文引用格式

王婧 , 张萌萌 , 张卓铅 , 曹文红 , 谭明堂 , 朱国萍 , 高加龙 , 林海生 , 郑惠娜 , 陈忠琴 . 基于纳米碳酸钙的牡蛎肽递送体系构建与结构表征及其消化特性研究[J]. 食品与发酵工业, 2025 , 51(19) : 238 -247 . DOI: 10.13995/j.cnki.11-1802/ts.042967

Abstract

Oyster bioactive peptides (OBP) exhibit multiple biological activities including antioxidant, hypoglycemic, and hypolipidemic effects.However, the poor stability of OBP in gastrointestinal environments significantly limits their efficacy.To enhance the stability of OBP and reduce the waste of oyster shell resources, this study first synthesized nano-calcium carbonate (CaCO3 NPs) from oyster shell.The CaCO3 NPs were then modified with sodium alginate (ALG), and the modification process was optimized.Subsequently, OBP was loaded onto ALG-CaCO3 NPs, followed by polyethylene glycol modification, to construct an OBP-CaCO3 NPs nano-system.Finally, the study investigated the structural stability, in vitro release, and digestive characteristics of the nano-system.The orthogonal experimental results indicated that the optimal modification process parameters for CaCO3 NPs were:ALG concentration of 5 mg/mL, temperature of 35 ℃, and reaction time of 2 h.The nano-system showed an average particle size of (288.7±7.6) nm and zeta potential of (-30.5±0.8) mV, achieving encapsulation efficiency of (35.69±2.22)% and loading capacity of (19.71±1.60)%.Fourier transform infrared spectroscopy analysis revealed that ALG modified CaCO3 NPs by forming an ‘egg box’ structure through the cross-linking of carboxyl group and Ca2+, while OBP were loaded inside the carrier through electrostatic effect.The results of in vitro release experiments showed that the cumulative release rate of the nano-system in the simulated gastric environment (pH 2.0) for 36 hours was only (40.31±0.63)%, while the release rate in the intestinal environment (pH 6.8) significantly increased to (93.12±3.76)%, indicating the nano-system had the characteristics of targeted intestinal slow release.Digestion stability experiments demonstrated that the nano-system could significantly improve the pancreatic lipase inhibitory activity of OBP, the inhibitory rate increased from (23.66±0.93)% to (54.73±1.37)% after gastric digestion, and from (4.75±0.38)% to (24.67±1.31)% after gastrointestinal digestion.This study provides theoretical support for the design of stable delivery systems for functional peptides, while offering a novel approach for the high-value utilization of oyster shell waste.

Key words： oyster peptides; nano-calcium carbonate; sodium alginate modification; nano delivery system; digestive stability

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