研究报告

米糠发酵液的抗氧化活性及其对秀丽隐杆线虫寿命的影响

  • 易翠平 ,
  • 张思颉 ,
  • 肖锦湘 ,
  • 赵波 ,
  • 李祖胤
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  • 1(湖南女子学院 社会发展学院,湖南 长沙,410004)
    2(长沙理工大学 食品与生物工程学院,湖南 长沙,410114)
第一作者:博士,教授(李祖胤讲师为通信作者,E-mail:lizuyin@hnwu.edu.cn)

收稿日期: 2024-11-07

  修回日期: 2025-01-23

  网络出版日期: 2025-11-21

基金资助

湖南省教育厅重点项目(22A0685)

Antioxidant activity of rice bran fermentation broth and its effect on the longevity of Caenorhabditis elegans

  • YI Cuiping ,
  • ZHANG Sijie ,
  • XIAO Jinxiang ,
  • ZHAO Bo ,
  • LI Zuyin
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  • 1(College of Social Development, Hunan Women’s University, Changsha 410004, China)
    2(School of Food Science and Bioengineering, Changsha University of Science and Technology, Changsha 410114, China)

Received date: 2024-11-07

  Revised date: 2025-01-23

  Online published: 2025-11-21

摘要

植物乳植杆菌具有改善食品的风味、营养品质及生物利用度等特点。为充分利用米糠资源,通过植物乳植杆菌发酵米糠来增强其抗氧化及抗衰老作用。该研究使用植物乳植杆菌对米糠进行发酵处理0、24、48 h,比较了发酵前后基本营养成分的变化,通过体外抗氧化活性和秀丽隐杆线虫模型分析米糠发酵液的生理活性。结果表明,发酵降低了米糠液中蛋白质和可溶性糖含量,发酵48 h后多酚含量是未发酵时的1.5倍,高达17.3 mg/100 g;在体外抗氧化活性中,米糠发酵液的羟自由基和DPPH清除率分别增加了10%和15%以上,使线虫的平均寿命增加2~3 d,有效延长热应激及H2O2氧化应激下线虫的平均寿命,分别延长了15%和25%。研究结果说明植物乳植杆菌发酵米糠有效提高了抗氧化及抗衰老作用,其作用主要体现在增加了多酚含量、提高自由基的清除率以及应激条件下延长了线虫的寿命,从而表明米糠发酵液具有一定的抗氧化、抗衰老能力,为植物乳植杆菌发酵米糠的功能性应用提供理论依据。

本文引用格式

易翠平 , 张思颉 , 肖锦湘 , 赵波 , 李祖胤 . 米糠发酵液的抗氧化活性及其对秀丽隐杆线虫寿命的影响[J]. 食品与发酵工业, 2025 , 51(21) : 70 -75 . DOI: 10.13995/j.cnki.11-1802/ts.041535

Abstract

Lactiplantibacillus plantarum has the characteristics of improving the flavor, nutritional quality and bioavailability of food.In order to make full use of rice bran resources, the antioxidant and anti-aging effects of rice bran fermented by L.plantarum were enhanced.In this study, rice bran was fermented using L.plantarum for 0, 24 and 48 h.The changes in the content of soluble sugars, proteins and polyphenols were compared before and after fermentation, and the physiological activity of rice bran fermentation solution was analyzed by means of antioxidant activity in vitro and Caenorhabditis elegans model.The results showed that fermentation reduced the protein and soluble sugar content of rice bran broth, and the polyphenol content reached 17.3 mg/100 g after 48 h of fermentation, 1.5 times that of before fermentation;in vitro antioxidant activity, the scavenging rates of hydroxyl radicals and DPPH in rice bran fermentation broth increased by more than 10% and 15%, respectively, which increased the average life span of C.elegans by 2-3 d, and effectively prolonged the average life span of C.elegans under heat stress and hydrogen peroxide oxidative stress by 15% and 25%, respectively.It indicated that L.plantarum fermented rice bran effectively improved the antioxidant and anti-aging effects, which were mainly reflected in the increased polyphenol content, improved free radical scavenging rate and prolonged C.elegans lifespan under stress conditions, thus indicating that the rice bran fermentation broth had a certain antioxidant and anti-aging ability, and providing a theoretical basis for the functional application of L.plantarum fermented rice bran.

参考文献

[1] KODAPE A, KODAPE A, DESAI R.Rice bran:Nutritional value, health benefits, and global implications for aflatoxin mitigation, cancer, diabetes, and diarrhea prevention[J].Food Chemistry, 2025, 464:141749.
[2] DEBI M R, WICHERT B A, LIESEGANG A.Anaerobic fermentation of rice bran with rumen liquor for reducing their fiber components to use as chicken feed[J].Heliyon, 2022, 8(4):e09275.
[3] WANCURA J H C, ALBARELLO M, HOLLAS S R, et al.Combined production of biofuel precursors, platform chemicals, and catalyst material from the integral processing of rice bran[J].Energy Conversion and Management, 2024, 317:118860.
[4] ZHANG B, ZHANG Y J, LI H Y, et al.A review on insoluble-bound phenolics in plant-based food matrix and their contribution to human health with future perspectives[J].Trends in Food Science & Technology, 2020, 105:347-362.
[5] NAJAMUDDIN U, GORJI S G, FITZGERALD M.Effect of post-milling process on the oxidation of the rice bran[J].Journal of Cereal Science, 2023, 111:103678.
[6] 曾锐, 汪卓, 陈菁, 等.乳酸菌发酵对海红米糠的结构和功能特性的影响[J].食品与发酵工业, 2023, 49(11):119-124.
ZENG R, WANG Z, CHEN J, et al.Effects of lactic acid bacteria fermentation on structure and functional properties of sea red rice bran[J].Food and Fermentation Industries, 2023, 49(11):119-124.
[7] 张慧娟, 冯雪佳, 曹欣然, 等.生物改性对脱脂米糠营养特性及功能特性的影响[J].中国油脂, 2021, 46(5):109-116.
ZHANG H J, FENG X J, CAO X R, et al.Effects of biological modification on nutritional and functional properties of defatted rice bran[J].China Oils and Fats, 2021, 46(5):109-116.
[8] PENG Z, QIAO B L, LI B, et al.Preparation, flavor component and antioxidant activity analysis of Lactiplantibacillus plantarum NCU137 fermented rice protein[J].Food Bioscience, 2024, 62:105017.
[9] 张夏秋, 刘丽娅, 王丽丽, 等.米根霉发酵米糠工艺优化及其益生活性研究[J].核农学报, 2020, 34(10):2280-2289.
ZHANG X Q, LIU L Y, WANG L L, et al.Optimization of the fermentation process of rice bran with Rhizopus oryzae and its probiotic activities[J].Journal of Nuclear Agricultural Sciences, 2020, 34(10):2280-2289.
[10] WANG Y M, ZHENG W J, XU B, et al.Comparison of rice bran characteristics fermented by mono- or di-strain probiotics based on omics technology[J].LWT, 2024, 209:116763.
[11] NEMOTO H, OTAKE M, MATSUMOTO T, et al.Prevention of tumor progression in inflammation-related carcinogenesis by anti-inflammatory and anti-mutagenic effects brought about by ingesting fermented brown rice and rice bran with Aspergillus oryzae (FBRA)[J].Journal of Functional Foods, 2022, 88:104907.
[12] 张嘉妮, 张晓轩, 王大毛, 等.乳酸菌发酵改善脱脂富硒米糠的抗氧化活性[J].食品科学, 2023, 44(24):146-154.
ZHANG J N, ZHANG X X, WANG D M, et al.Improvement of antioxidant activity of defatted selenium-enriched rice bran by lactic acid bacteria fermentation[J].Food Science, 2023, 44(24):146-154.
[13] LIN Y G, LIN C X, CAO Y, et al.Caenorhabditis elegans as an in vivo model for the identification of natural antioxidants with anti-aging actions[J].Biomedicine & Pharmacotherapy, 2023, 167:115594.
[14] GUO X H, XIN Q C, WEI P, et al.Antioxidant and anti-aging activities of Longan crude and purified polysaccharide (LP-A) in nematode Caenorhabditis elegans[J].International Journal of Biological Macromolecules, 2024, 267:131634.
[15] HU Q, LIU Z G, GUO Y J, et al.Antioxidant capacity of flavonoids from Folium Artemisiae Argyi and the molecular mechanism in Caenorhabditis elegans[J].Journal of Ethnopharmacology, 2021, 279:114398.
[16] MUDD N, MARTIN-GONZALEZ F S, FERRUZZI M, et al.In vivo antioxidant effect of edible cricket (Gryllodes sigillatus) peptides using a Caenorhabditis elegans model[J].Food Hydrocolloids for Health, 2022, 2:100083.
[17] PATANANAN A N, BUDENHOLZER L M, ESKIN A, et al.Ethanol-induced differential gene expression and acetyl-CoA metabolism in a longevity model of the nematode Caenorhabditis elegans[J].Experimental Gerontology, 2015, 61:20-30.
[18] YI C P, XIE L, CAO Z F, et al.Effects of rice bran fermented with Lactobacillus plantarum on palatability, volatile profiles, and antioxidant activity of brown rice noodles[J].International Journal of Food Science & Technology, 2022, 57(8):5048-5056.
[19] LIN S M, ZHANG X X, WANG J R, et al.Effect of lactic acid bacteria fermentation on bioactive components of black rice bran (Oryza sativa L.) with different milling fractions[J].Food Bioscience, 2024, 58:103684.
[20] 杨庆华, 张亚飞, 田晓静, 等.谷物发酵产品的营养功能提升与益生功能研究进展[J].食品与发酵工业, 2022, 48(17):304-312.
YANG Q H, ZHANG Y F, TIAN X J, et al.Research progress on the improvement of nutritional function and probiotic performance of cereal fermented products[J].Food and Fermentation Industries, 2022, 48(17):304-312.
[21] 严静, 薛秋艳, 王旸, 等.发酵米荞对高脂肪秀丽隐杆线虫的降脂及抗氧化作用[J].食品工业科技, 2023, 44(6):8-15.
YAN J, XUE Q Y, WANG Y, et al.Hypolipidemic and antioxidant effects of fermented rice buckwheat on high-fat Caenorhabditis elegans[J].Science and Technology of Food Industry, 2023, 44(6):8-15.
[22] 卢翔, 张翔宇, 李晨.小米糠多肽抗氧化活性及对秀丽隐杆线虫延寿作用初析[J].山西农业科学, 2022, 50(11):1542-1548.
LU X, ZHANG X Y, LI C.Antioxidant activity of polypeptides from millet bran and its lifespan- prolonging effect on Caenorhabditis elegans[J].Journal of Shanxi Agricultural Sciences, 2022, 50(11):1542-1548.
[23] PALLAUF K, DUCKSTEIN N, RIMBACH G.A literature review of flavonoids and lifespan in model organisms[J].Proceedings of the Nutrition Society, 2017, 76(2):145-162.
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