食品与发酵工业 >

2025 , Vol. 51 >Issue 21: 232 - 242

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041800

研究报告

羧甲基化、硒化黄绿蜜环菌多糖结构表征及生物活性研究

  • 符娜 ,
  • 时文盼 ,
  • 李静洁 ,
  • 蔺永刚 ,
  • 王俊龙
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  • 1(伊犁师范大学,新疆生物质资源清洁转化与高值化利用重点实验室,新疆 伊宁,835000)
    2(伊犁州中医医院,新疆 伊宁,835000)
第一作者：硕士,实验师(蔺永刚实验师和王俊龙实验师为通信作者,E-mail:869294906@qq.com;974881461@qq.com)

收稿日期: 2024-12-08

  修回日期: 2025-03-14

  网络出版日期: 2025-11-21

基金资助

2023年伊犁州科技计划项目(YJC2023A15)

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Structural characterization and bioactivity of carboxymethylated and selenated Armillaria luteo-virens polysaccharide

  • FU Na ,
  • SHI Wenpan ,
  • LI Jingjie ,
  • LIN Yonggang ,
  • WANG Junlong
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  • 1(Key Laboratory of Clean Transformation and High-value Utilization of Biomass Resources, Yili Normal University, Yining 835000, China)
    2(Traditional Chinese Medicine Hospital of Yili Prefecture, Yining 835000, China)

Received date: 2024-12-08

  Revised date: 2025-03-14

  Online published: 2025-11-21

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摘要

基于黄绿蜜环菌多糖(Armillaria luteo-virens polysaccharide,ALVP)的天然生物活性,该研究以ALVP为原料,通过羧甲基化和硒化修饰提高其抗氧化和降血糖的能力。实验采用碱性氯乙酸法和亚硒酸钠法分别制备羧甲基化黄绿蜜环菌多糖CM-ALVP和硒化黄绿蜜环菌多糖Se-ALVP,采用光谱及色谱分析技术对修饰前后多糖的结构进行表征,通过测定修饰前后多糖对DPPH自由基、ABTS阳离子自由基、羟自由基的清除能力及Fe3+还原力,以及对α-葡萄糖苷酶和α-淀粉酶的抑制能力评价其抗氧化、降血糖能力。结果表明,3种多糖均由岩藻糖、鼠李糖、甘露糖、半乳糖、葡萄糖组成的具有吡喃糖的杂多糖,但修饰后单糖摩尔比发生变化。与ALVP相比,修饰后多糖CM-ALVP和Se-ALVP的水溶性、热稳定性增强,分子质量均从23.2 kDa增加到23.3 kDa,但三股螺旋构象及物相结构未发生变化。傅里叶红外光谱在1 617 cm-1和1 411 cm-1出现CO基团的对称和不对称伸缩振动峰,在762 cm-1出现SeO的特征吸收峰,此外,核磁共振波谱在δ 4.46 ppm和δ 4.45 ppm出现的信号峰,表明羧甲基化和硒化修饰成功。抗氧化、降血糖活性结果显示,与ALVP相比,在质量浓度为8 mg/mL时,ALVP、CM-ALVP和Se-ALVP对DPPH自由基的半抑制浓度(IC50)分别为3.05、1.76、1.35 mg/mL,对ABTS阳离子自由基的IC50分别为5.72、1.79、0.96 mg/mL,对羟自由基的IC50分别为4.00、2.00、2.00 mg/mL,对Fe3+还原能力分别为0.89、0.96、1.05 AU,对α-葡萄糖苷酶的抑制率分别为45.56%、49.12%、54.11%,对α-淀粉酶的抑制率分别为42.44%、48.77%、55.78%。表明羧甲基化、硒化修饰能够提高ALVP的抗氧化、降血糖活性。该研究为ALVP的深度开发利用提供理论依据和数据支撑。

关键词： 黄绿蜜环菌多糖; 羧甲基化修饰; 硒化修饰; 理化性质; 生物活性

本文引用格式

符娜 , 时文盼 , 李静洁 , 蔺永刚 , 王俊龙 . 羧甲基化、硒化黄绿蜜环菌多糖结构表征及生物活性研究[J]. 食品与发酵工业, 2025 , 51(21) : 232 -242 . DOI: 10.13995/j.cnki.11-1802/ts.041800

Abstract

Based on the natural bioactivities of the polysaccharides from Armillaria luteo-virens polysaccharide (ALVP), the study was conducted to improve the antioxidant and hypoglycemic abilities of ALVP by carboxymethylation and selenylation modifications as raw materials.CM-ALVP and Se-ALVP were synthesized using the alkaline chloroacetic acid method and the sodium selenite method, respectively, from Armillaria luteo-virens polysaccharide (ALVP).The structural characteristics of the polysaccharides before and after modification were elucidated through spectroscopic and chromatographic analyses.The antioxidant and hypoglycemic capacities of the modified polysaccharides were evaluated by assessing their scavenging abilities against DPPH free radicals, ABTS cationic radicals, and hydroxyl radicals, as well as their Fe3+ reducing power.Additionally, their inhibitory effects on α-glucosidase and α-amylase were determined.Results indicated that all three polysaccharides were heteropolysaccharides composed of fucose, rhamnose, mannose, galactose, and glucose, exhibiting a pyranose structure.However, it was observed that the molar ratios of the monosaccharides changed following the modifications.Compared to ALVP, the modified polysaccharides CM-ALVP and Se-ALVP showed enhanced water solubility and thermal stability, with molecular weights increasing from 23.2 kDa to 23.3 kDa for both modifications.No changes were observed in the triple helical conformation or the phase structure.Fourier transform infrared spectroscopy revealed symmetric and asymmetric stretching vibrations of C＝O groups at 1 617 cm-1 and 1 411 cm-1, respectively, and a characteristic absorption peak of SeO at 762 cm-1.Furthermore, nuclear magnetic resonance spectroscopy showed signal peaks at δ 4.46 ppm and δ 4.45 ppm, indicating successful carboxymethylation and selenylation modifications.The antioxidant and hypoglycemic activities demonstrated that, at a mass concentration of 8 mg/mL, the half-maximal inhibitory concentrations (IC50) of ALVP, CM-ALVP, and Se-ALVP against DPPH radicals were 3.05, 1.76, and 1.35 mg/mL, respectively, against ABTS radicals, the IC50 values were 5.72, 1.79, and 0.96 mg/mL, for hydroxyl radicals, the IC50 values were 4.00, 2.00, and 2.00 mg/mL, and the Fe3+ reducing capacities were 0.89, 0.96, and 1.05 AU, respectively.In terms of inhibitory rates against α-glucosidase, the values were 45.56%, 49.12%, and 54.11%, while the inhibitory rates against α-amylase were 42.44%, 48.77%, and 55.78%.These findings suggest that carboxymethylation and selenylation modifications can enhance the antioxidant and hypoglycemic activities of polysaccharides derived from yellow-green honey ring fungus.This study provides a theoretical foundation and data support for the further development and utilization of polysaccharides from Armillaria luteo-virens.

Key words： Armillaria luteo-virens polysaccharide(ALVP); carboxymethylation modification; selenation modification; physicochemical properties; biological activity

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