食品与发酵工业 >

2025 , Vol. 51 >Issue 21: 243 - 253

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041887

研究报告

pH偏移预处理对核桃蛋白-羧甲基壳聚糖复合颗粒的影响及在高内相Pickering乳液中的应用

  • 贾立奥 ,
  • 王颖颖 ,
  • 寇锟 ,
  • 姚飞 ,
  • 殷丽君 ,
  • 陈复生 ,
  • 郭兴凤
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  • 1(河南工业大学 粮油食品学院,河南 郑州,450001)
    2(中国农业大学 食品科学与营养工程学院,北京,100083)
    3(中原食品实验室,河南 漯河,462333)
第一作者：硕士研究生(殷丽君教授和陈复生教授为共同通信作者,E-mail:ljyin@cau.edu.cn;fushengc@haut.edu.cn)

收稿日期: 2024-12-17

  修回日期: 2025-03-25

  网络出版日期: 2025-11-21

基金资助

“十四五”国家重点研发计划项目(2023YFD2100402)

收起

Effect of pH migration pretreatment on walnut protein-carboxymethyl chitosan composite particles and its application in high internal phase Pickering emulsion

  • JIA Li’ao ,
  • WANG Yingying ,
  • KOU Kun ,
  • YAO Fei ,
  • YIN Lijun ,
  • CHEN Fusheng ,
  • GUO Xingfeng
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  • 1(College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001,China)
    2(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083,China)
    3(Food Laboratory of Zhongyuan, Luohe 462333,China)

Received date: 2024-12-17

  Revised date: 2025-03-25

  Online published: 2025-11-21

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摘要

为了提升核桃蛋白的乳化性能,该文研究了pH偏移预处理对核桃蛋白-羧甲基壳聚糖复合物结构、界面吸附特性和乳化性能的影响,并探究了不同pH偏移预处理复合物稳定高内相Pickering乳液的潜力。结果表明,pH偏移预处理使核桃蛋白-羧甲基壳聚糖复合物结构变得有序,粒径减小,湿润性得到提高,从而降低了其在界面吸附所需要的能量,表现出良好的乳化性能。其中pH值为11偏移预处理条件下,复合物的粒径较小(561.94 nm),电位值最大(-47.51 mV),湿润性最好,接触角接近90°(91.2°),稳定界面所需能量最低,乳化性(180.48 m2/g)和乳化稳定性(99.03%)最高,显示出巨大的稳定乳液的潜力,其稳定的乳液显示出更窄的粒径分布和良好的储存稳定性,乳液在保存4个月以上仍未出现分层。因此,pH偏移可以改善核桃蛋白的结构和界面性质,提升其乳化性能。研究结果可为核桃蛋白在乳液体系的应用提供参考。

关键词： 核桃蛋白; 羧甲基壳聚糖; pH偏移; 乳液; 高内相乳液

本文引用格式

贾立奥 , 王颖颖 , 寇锟 , 姚飞 , 殷丽君 , 陈复生 , 郭兴凤 . pH偏移预处理对核桃蛋白-羧甲基壳聚糖复合颗粒的影响及在高内相Pickering乳液中的应用[J]. 食品与发酵工业, 2025 , 51(21) : 243 -253 . DOI: 10.13995/j.cnki.11-1802/ts.041887

Abstract

To enhance the emulsifying properties of walnut protein isolate (WPI), this study investigated the effects of pH shift pretreatment on the structure, interfacial adsorption characteristics, and emulsifying properties of walnut protein-carboxymethyl chitosan (CMCS) complexes, and explored the potential of different pH shift pretreatment complexes in stabilizing high internal phase Pickering emulsions.The results indicated that pH shift pretreatment made the structure of the WPI-CMCS complex more ordered, reduced particle size, and improved wettability, thereby lowering the energy required for interfacial adsorption and exhibiting good emulsification performance.Under the pH 11 shift pretreatment conditions, the WPI-CMCS complex had a smaller particle size (561.94 nm), the highest zeta potential (-47.51 mV), the best wettability, and a contact angle close to 90° (91.2°).The energy required to stabilize the interface was the lowest, with the highest emulsifying activity (180.48 m2/g) and emulsifying stability (99.03%), demonstrating significant potential for stable emulsions.The stable emulsions exhibited a narrower particle size distribution and good storage stability, with no phase separation observed even after being stored for more than four months.Therefore, pH shift could improve the structure and interfacial properties of the walnut protein, enhancing its emulsifying performance.The research results can provide a reference for the application of walnut protein in emulsion systems.

Key words： walnut protein; carboxymethyl chitosan; pH shift; emulsion; high internal phase emulsion

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