食品与发酵工业 >

2025 , Vol. 51 >Issue 21: 338 - 345

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042121

研究报告

原花青素接枝羧甲基壳聚糖对羊肚菌的保鲜效果研究

  • 陈凤 ,
  • 赵旭东 ,
  • 任洁清 ,
  • 日达古丽·肉孜托合提 ,
  • 裴龙英
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  • 1(新疆黑木耳工程技术研究中心,新疆 阿克苏,843100)
    2(塔里木大学 食品科学与工程学院,新疆 阿拉尔,843300)
    3(新疆理工学院,食品安全与营养实验教学中心,新疆 阿克苏,843100)
第一作者：本科生(裴龙英副教授为通信作者,E-mail:peilongy@163.com)

收稿日期: 2025-01-13

  修回日期: 2025-03-26

  网络出版日期: 2025-11-21

基金资助

新疆维吾尔自治区自然科学基金资助项目(2024D01C271);新疆维吾尔自治区大学生创新创业项目(S202413558004);羊肚菌栽培技术服务(XJLGHX2024010);植物资源理化特性及利用研究(XJLGHX2024060)

收起

Research on the preservation effect of proanthocyanidins grafted with carboxymethyl chitosan on Morchella

  • CHEN Feng ,
  • ZHAO Xudong ,
  • REN Jieqing ,
  • RIDAGULI·Rouzituoheti ,
  • PEI Longying
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  • 1(Xinjiang Black Fungus Engineering and Technology Research Center, Aksu 843100, China)
    2(College of Food Science and Engineering, Tarim University, Alaer 843300, China)
    3(Food Safety and Nutrition Experimental Teaching Demonstration Center, Xinjiang Institute of Technology, Aksu 843100, China)

Received date: 2025-01-13

  Revised date: 2025-03-26

  Online published: 2025-11-21

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摘要

为提高羧甲基壳聚糖(carboxymethyl chitosan,CMCS)的抗氧化能力,该研究以CMCS和原花青素(proanthocyanidins,PA)为原料,采用自由基介导接枝法,通过不同物质的量比的CMCS和PA反应,制备了具有强抗氧化性的原花青素-羧甲基壳聚糖接枝共聚物(proanthocyanidins-grafted-carboxymethyl chitosan,PA-CMCS)。对PA-CMCS进行了结构表征和性能测定,并考察了4 ℃贮藏14 d过程中PA-CMCS对羊肚菌涂膜处理的保鲜效果。结果表明,CMCS与PA的物质的量比为1∶0.3时达到接枝饱和,结构中出现苯环,表明接枝成功。体外抗氧化实验结果表明,在0.01～0.2 mg/mL质量浓度内,PA-CMCS对DPPH自由基和ABTS阳离子自由基的最大清除率分别为75.14%和65.56%,远高于CMCS的清除率,表明接枝共聚物的抗氧化能力显著提高。相比于CMCS,PA-CMCS表现出更好的抗氧化和抑菌性能,且对羊肚菌的保鲜效果更好。该研究对拓宽CMCS在食品领域的应用具有重要意义,并为新型果蔬保鲜材料的开发提供了一定的科学依据和理论基础。

关键词： 羧甲基壳聚糖; 原花青素; 接枝; 羊肚菌; 保鲜

本文引用格式

陈凤 , 赵旭东 , 任洁清 , 日达古丽·肉孜托合提 , 裴龙英 . 原花青素接枝羧甲基壳聚糖对羊肚菌的保鲜效果研究[J]. 食品与发酵工业, 2025 , 51(21) : 338 -345 . DOI: 10.13995/j.cnki.11-1802/ts.042121

Abstract

To enhance the antioxidant capacity of carboxymethyl chitosan (CMCS), this study took carboxymethyl chitosan and proanthocyanidins (PA) as raw materials.A free radical-mediated grafting method was adopted to prepare proanthocyanidins-grafted-carboxymethyl chitosan (PA-CMCS) with strong antioxidant properties through the reaction of CMCS and PA at different molar ratios.The structure of PA-CMCS was characterized, and its properties were determined.Also, the preservation effect of PA-CMCS on the coating treatment of Morchella during 14-day storage at 4 ℃ was investigated.Results showed that the grafting reached saturation when the molar ratio of CMCS to PA was 1∶0.3.The appearance of a benzene ring in the structure indicated successful grafting.The results of in vitro antioxidant experiments showed that within the mass concentration range of 0.01-0.2 mg/mL, the maximum scavenging rates of PA-CMCS for DPPH and ABTS cationic free radicals were 75.17% and 64.56%, much higher than those of CMCS, indicating a significant improvement in the antioxidant capacity of the graft copolymer.Compared with CMCS, PA-CMCS exhibited better antioxidant and antibacterial properties and showed a more favorable preservation effect on Morchella.This study is of great significance to broaden the application of CMCS in the food field and provides a scientific basis and theoretical foundation for the development of new fruit and vegetable preservation materials.

Key words： carboxymethyl chitosan; proanthocyanidins; grafting; Morchella; preservation

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