食品与发酵工业 >

2025 , Vol. 51 >Issue 21: 372 - 382

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042257

分析与检测

基于理化分析与代谢组学的甘肃河西走廊赤霞珠葡萄最佳采收期

  • 李怡瑶 ,
  • 吕转转 ,
  • 翦祎 ,
  • 程晓燕 ,
  • 陈建军 ,
  • 张竹林 ,
  • 张波
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  • 1(甘肃农业大学 食品科学与工程学院,甘肃省葡萄酒产业技术研发中心,甘肃 兰州,730070)
    2(烟台市牟平区检验检测中心,山东 烟台,264100)
    3(甘肃农业大学 理学院,甘肃 兰州,730070)
    4(甘肃国风庄园葡萄酒业有限责任公司,甘肃 张掖,734000)
第一作者：硕士研究生(张波教授为通信作者,E-mail:zb692002@163.com)

收稿日期: 2025-01-24

  修回日期: 2025-04-01

  网络出版日期: 2025-11-21

基金资助

甘肃省陇原青年英才项目(LYYC-2024-03);甘肃省葡萄酒产业发展专项资金项目(GSPTJZX-2020-4,2017010);甘肃省科技计划项目重点研发计划(20YF3NA017)

收起

Optimal harvest time of Cabernet Sauvignon grapes in the Hexi Corridor of Gansu based on physicochemical analysis and metabolomics

  • LI Yiyao ,
  • LYU Zhuanzhuan ,
  • JIAN Yi ,
  • CHENG Xiaoyan ,
  • CHEN Jianjun ,
  • ZHANG Zhulin ,
  • ZHANG Bo
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  • 1(College of Food Science and Engineering, Gansu Agricultural University, Grape Wine Industry Technology Research and Development Center of Gansu Province, Lanzhou 730070, China)
    2(Muping District Inspection and Testing Center, Yantai 264100, China)
    3(College of Science, Gansu Agricultural University, Lanzhou 730070, China)
    4(Gansu Guofeng Winery Co.Ltd., Zhangye 734000, China)

Received date: 2025-01-24

  Revised date: 2025-04-01

  Online published: 2025-11-21

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摘要

酿酒葡萄的合理采收期选择对葡萄酒的质量控制和形成至关重要。然而,传统的理化分析方法无法全面评估葡萄的最佳采收期。为此,该研究选取甘肃河西走廊产区种植的赤霞珠葡萄为研究对象,在花后83~101 d阶段对葡萄样品的理化指标和多酚含量进行测定。同时,应用超高效液相色谱串联三重四级杆质谱技术对花后91、93、96 d的葡萄样品进行了广泛靶向代谢组学分析,进一步探讨最佳采收期。研究结果显示,赤霞珠葡萄果实中共检测到1 811种代谢物,特别是黄酮类、氨基酸及其衍生物和酚酸类物质的变化显著。通过综合理化指标、多酚含量和代谢组学分析,推测花后91~93 d为最佳采收期。此外,KEGG分析进一步揭示,差异显著的代谢物主要参与次生代谢途径,特别是黄酮和黄酮醇代谢,并初步推测儿茶素、表儿茶素、没食子儿茶素、二甲花翠素-3-O-葡萄糖苷、花青素-3-O-(6″-O-咖啡酰)-葡萄糖苷、甲基花青素-3-O-(6″-O-咖啡酰)-葡萄糖苷、山奈酚-3-O-葡萄糖苷、山奈酚-3-O-半乳糖苷和异鼠李素-3-O-葡萄糖苷可能是判断最佳采收期的重要指标。该研究为甘肃河西走廊产区赤霞珠葡萄合理采收期的确定提供了一定的数据支持和理论依据。

关键词： 赤霞珠; 采收期; 理化指标; 多酚; 广泛靶向代谢组学

本文引用格式

李怡瑶 , 吕转转 , 翦祎 , 程晓燕 , 陈建军 , 张竹林 , 张波 . 基于理化分析与代谢组学的甘肃河西走廊赤霞珠葡萄最佳采收期[J]. 食品与发酵工业, 2025 , 51(21) : 372 -382 . DOI: 10.13995/j.cnki.11-1802/ts.042257

Abstract

The selection of the optimal harvest time for wine grapes is crucial for quality control and the development of wine.However, traditional physicochemical analysis methods are insufficient for comprehensively evaluating the optimal harvest time for grapes.The study selected Cabernet Sauvignon grapes cultivated in the Hexi Corridor region of Gansu as the research subject to address this issue.The physicochemical parameters and polyphenol content of grape samples were measured from 83 to 101 days post-flowering.Meanwhile, ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry was used for comprehensive targeted metabolomics analysis of grape samples at 91, 93, and 96 days post-flowering, further exploring the optimal harvest time.The results showed that 1 811 metabolites were detected in Cabernet Sauvignon grapes, with significant changes observed in flavonoids, amino acids and their derivatives, and phenolic acids.By integrating physicochemical parameters, polyphenol content, and metabolomics analysis, it was inferred that the optimal harvest time is between 91 and 93 days post-flowering.Furthermore, KEGG analysis revealed that the significantly altered metabolites are primarily involved in secondary metabolic pathways, especially flavonoid and flavonol metabolism.It was also preliminarily suggested that catechin, epicatechin, gallic acid catechin, malvidin-3-O-glucoside,cyanidin-3-O-(6″-O-caffeoyl)-glucoside,peonidin-3-O-(6″-O-caffeoyl)-glucoside,kaempferol-3-O-glucoside, kaempferol-3-O-galactoside, and isoquercitrin-3-O-glucoside may be key indicators for determining the optimal harvest time.This study provides valuable data support and a theoretical basis for determining the optimal harvest time of Cabernet Sauvignon grapes in the Hexi Corridor region of Gansu.

Key words： Cabernet Sauvignon; harvest timing; physicochemical indexes; polyphenols; broad-targeted metabolomics

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