食品与发酵工业 >

2025 , Vol. 51 >Issue 22: 119 - 127

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042459

研究报告

乙酸盐转运蛋白PepatA正向调控扩展青霉中孢子发生和毒素积累

  • 王艳玲 ,
  • 高洁 ,
  • 曹丽丽 ,
  • 黄彦琪 ,
  • 朱锦芬
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  • (兰州理工大学 生命科学与工程学院,甘肃 兰州,730050)
第一作者:博士,副教授(通信作者,E-mail:wangyanling2003359@126.com)

收稿日期: 2025-02-21

  修回日期: 2025-04-01

  网络出版日期: 2025-12-15

基金资助

国家自然科学基金项目(31760494);2025年甘肃省科技专员项目(25CXGA053)

收起

Acetate transporter protein PepatA regulates sporulation and toxin accumulation in Penicillium expansum

  • WANG Yanling ,
  • GAO Jie ,
  • CAO Lili ,
  • HUANG Yanqi ,
  • ZHU Jinfen
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  • (School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China)

Received date: 2025-02-21

  Revised date: 2025-04-01

  Online published: 2025-12-15

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摘要

乙酸盐转运蛋白家族作为乙酸盐摄取和分泌的重要载体,在菌体生长发育中起着关键作用。为了探究乙酸盐转运蛋白PepatA在扩展青霉中发挥的作用和生物学功能,该研究采用同源重组策略成功构建PepatA的缺失和回复突变菌株,并对突变菌株的生长、孢子发生、致病性和毒素积累进行分析。结果显示,PepatA缺失菌株菌落表型与野生型及回复菌株基本一致,无明显变化;PepatA缺失菌株孢子萌发率和芽管长度与野生型及回复菌株相比有所减少;产孢量和孢子梗数量显著减少,孢子生成周期明显滞后;PepatA缺失菌株致病性减弱,棒曲霉素(patulin,PAT)积累显著下降。进一步采用分子对接结合点突变构建突变菌株(PatA-F53A),检测菌株生长及毒素积累变化情况。结果显示,乙酸盐转运蛋白PepatA与底物分子间-甲酚存在1个重要活性位点Phe53;突变菌株PatA-F53A生长表型及产孢不受影响,但PAT积累显著下降。综上,乙酸盐转运蛋白PepatA正向调控孢子发生和毒素积累,且Phe53是PepatA蛋白与间-甲酚结合的一个重要位点,这将为PAT的防控提供一个潜在靶标位点。

关键词： 扩展青霉; 乙酸盐转运蛋白; 毒素积累; 孢子发生; 分子对接

本文引用格式

王艳玲 , 高洁 , 曹丽丽 , 黄彦琪 , 朱锦芬 . 乙酸盐转运蛋白PepatA正向调控扩展青霉中孢子发生和毒素积累[J]. 食品与发酵工业, 2025 , 51(22) : 119 -127 . DOI: 10.13995/j.cnki.11-1802/ts.042459

Abstract

Acetate transporter proteins, as important carriers for acetate uptake and secretion, play a key role in the growth and development of fungi.To investigate the role and biological function of acetate transporter protein PepatA in Penicillium expansum, a homologous recombination strategy was used to construct deletion and complementary strains of PepatA, and analyzed the growth, sporulation, pathogenicity, and toxin accumulation of the mutant strains.Results showed that compared to wild-type and complementary strains, the colony phenotype of the PepatA strain did not change significantly.However, the PepatA strain showed a reduced spore germination rate and germ tube length, decreased spore production and spore peduncle number, a delayed sporulation cycle, weakened pathogenicity, and diminished patulin accumulation.The mutant strain (PatA-F53A) was constructed by molecular docking combined with point mutation, and the changes in growth and toxin accumulation were assessed.Results showed that PatA and the substrate molecule m-Cresol had an important active site, Phe53.Although the growth phenotype and spore production of the PatA-F53A strain were not affected, patulin accumulation was significantly decreased.In summary, the acetate transporter protein PepatA positively regulated sporulation and toxin accumulation, and Phe53 was an important site for the binding of the PepatA protein to m-Cresol, providing a potential target site for the prevention and control of patulin.

Key words： Penicillium expansum; acetate transporter protein; toxin accumulation; sporogenesis; molecular docking

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