从中国西藏自治区一名健康婴儿肠道中分离出的格氏乳杆菌G098(Lactobacillus gasseri G098)具有良好的益生特性,包括对人工胃肠液和胆盐的强耐受性。然而,在培养过程中其细胞密度低限制了其在工业生产中的潜力。为解决这一限制,该研究利用基因组学信息,快速筛选生长因子,优化了L.gasseri G098的培养基。全基因组测序结果显示,L.gasseri G098基因组全长1 991 131 bp,GC含量为35.30%,编码基因1 982个。基因组数据的综合代谢途径分析确定了对生长至关重要的特定氨基酸和维生素,并将其纳入到了随后的培养基优化实验中。最终的优化培养基配方为:38.00 g乳糖、62.66 g鱼蛋白胨、31.33 g胰蛋白胨、5.75 g磷酸氢二钾、14.27 g无水乙酸钠、5.75 g柠檬酸钠、0.01 g FeSO4·7H2O、1.00 g吐温80,溶于1 000 mL蒸馏水中。在优化后的培养基中培养L.gasseri G098,活菌数达到5.48×109 CFU/mL,是未优化培养基的5.1倍。
Lactobacillus gasseri G098, isolated from the intestine of a healthy infant in the Tibet, China, demonstrates excellent probiotic properties, including strong tolerance to artificial gastrointestinal fluids and bile salts.However, its low cell density during cultivation limits its potential for industrial production.To address this limitation, this study optimized the culture medium for L.gasseri G098 by genomic information for rapid screening of growth factors.Whole-genome sequencing analysis revealed that the genome of L.gasseri G098 spans 1 991 131 bp, with a GC content of 35.30%, and encodes 1 982 genes.Comprehensive metabolic pathway analysis of the genomic data identified specific amino acids and vitamins critical for growth, which were incorporated into subsequent medium optimization experiments.The final optimized medium formulation consisted of the following components (per liter):38.00 g lactose, 62.66 g fish peptone, 31.33 g tryptone, 5.75 g dipotassium phosphate, 14.27 g anhydrous sodium acetate, 5.75 g sodium citrate, 0.01 g FeSO4·7H2O, and 1.00 g Tween 80, dissolved in 1 000 mL of distilled water.Cultivation of L.gasseri G098 in this optimized medium resulted in a viable cell count of 5.48×109 CFU/mL, representing a 5.1-fold increase compared to the unoptimized medium.
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