食品与发酵工业 >

2025 , Vol. 51 >Issue 22: 342 - 351

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042332

研究报告

柴达木双层环伞菌多糖合成的调控技术及其动力学模型构建研究

  • 邱松林 ,
  • 邓莹莹 ,
  • 童彩玲 ,
  • 焦迎春
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  • 1(厦门海洋职业技术学院 海洋生物学院,海洋食品营养安全及精深加工福建省高等学校应用技术工程中心,福建 厦门,361100)
    2(青海大学 农牧学院,青海 西宁,810016)
第一作者:硕士,副教授(焦迎春教授为通信作者,E-mail:280717062@qq.com)

收稿日期: 2025-02-14

  修回日期: 2025-04-05

  网络出版日期: 2025-12-15

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Regulation technology and construction of kinetics model of polysaccharide synthesis of Agaricus bitorquis (Quél.) Sacc.Chaidam

  • QIU Songlin ,
  • DENG Yingying ,
  • TONG Cailing ,
  • JIAO Yingchun
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  • 1(School of Marine Biology, Xiamen Ocean Vocational College, Applied Technology Engineering Center of Fujian Provincial Higher Education for Marine Food Nutrition Safety and Advanced Processing, Xiamen 361100, China)
    2(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

Received date: 2025-02-14

  Revised date: 2025-04-05

  Online published: 2025-12-15

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摘要

该文通过向柴达木双层环伞菌发酵体系中添加黑果枸杞花色苷粗提物,研究其对菌体生长和胞外多糖(exopoly saccharides,EPS)合成的影响,优化了花色苷的添加方式和发酵温度工艺,同时对调控体系中的残糖(还原糖)含量、pH及溶解氧的动态变化进行研究。研究表明,相较于空白对照组,采用半流加黑果枸杞花色苷粗提物(发酵0~36 h流加,速率0.83 mL/min,总添加量1 800 mL),变温培养(0~36 h为23 ℃,36~96 h为25 ℃,96~120 h为23 ℃)方式,菌丝生物量提高89.04%、多糖产量增加65.46%。从构建的菌体生长、EPS产量及底物消耗的动力学模型可知,调控组模型拟合曲线的相关系数均＞0.93,拟合情况良好,说明模型能很好地反映发酵过程规律。因此,黑果枸杞花色苷能够显著促进柴达木双层环伞菌生长与EPS的合成。该研究为青藏高原特色食用菌的规模化开发与应用提供理论支持。

关键词： 柴达木双层环伞菌; 多糖合成; 黑果枸杞花色苷; 调控技术; 动力学模型

本文引用格式

邱松林 , 邓莹莹 , 童彩玲 , 焦迎春 . 柴达木双层环伞菌多糖合成的调控技术及其动力学模型构建研究[J]. 食品与发酵工业, 2025 , 51(22) : 342 -351 . DOI: 10.13995/j.cnki.11-1802/ts.042332

Abstract

The effects of anthocyanin extracts from Lycium ruthenicum Murr on the growth of mycelium and synthesis of extracellular polysaccharide (EPS) were studied by adding them into the fermentation system of Agaricus bitorquis (Quél.) Sacc.Chaidam.The addition method of anthocyanins and fermentation temperature were optimized, and the dynamic changes of residual sugar (reducing sugar) content, pH value and dissolved oxygen in the control system were studied.The study showed that compared with the blank control group, the extraction of anthocyanins of Lycium ruthenicum Murr was added in half flow (fermentation 0-36 h flow, the rate of 0.83 mL/min, the total amount of 1 800 mL) and cultured at variable temperature (23 ℃ for 0-36 h, 25 ℃ for 36-96 h, 23 ℃ for 96-120 h), mycelial biomass increased by 89.04% and polysaccharide yield increased by 65.46%.According to the established dynamic models of cell growth, EPS production and substrate consumption, the correlation coefficients of the fitting curves of the regulation group model were all >0.93, indicating that the model could well reflect the fermentation process.Therefore, the anthocyanins of Lycium ruthenicum Murr can significantly promote the growth and synthesis of EPS of Agaricus bitorquis (Quél.) Sacc.Chaidam.This research provides theoretical support for the large-scale development and application of the characteristic edible fungi on the Qinghai-Tibet Plateau.

Key words： Agaricus bitorquis (Quél.) Sacc.Chaidam; polysaccharide synthesis; Lycium ruthenicum Murr; regulation technique; kinetic model

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