食品与发酵工业 >

2025 , Vol. 51 >Issue 22: 411 - 419

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043507

综述与专题评论

抗菌肽微生物异源表达系统和表达策略优化研究进展

  • 李玉珍 ,
  • 肖怀秋 ,
  • 王斌 ,
  • 廖琼 ,
  • 刘畅宇 ,
  • 唐旭阳
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  • 1(湖南化工职业技术学院 化学工程学院,湖南 株洲,412000)
    2(湖南化工职业技术学院 制药与生物工程学院,湖南 株洲,412000)
第一作者:硕士,副教授(肖怀秋教授为通信作者,E-mail:xiaohuaiqiu@163.com)

收稿日期: 2025-06-10

  修回日期: 2025-07-15

  网络出版日期: 2025-12-15

基金资助

湖南省自然科学基金科教联合基金项目(2022JJ60046)

收起

Research advances on heterologous expression systems and expression strategies optimization for antimicrobial peptides

  • LI Yuzhen ,
  • XIAO Huaiqiu ,
  • WANG Bin ,
  • LIAO Qiong ,
  • LIU Changyu ,
  • TANG Xuyang
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  • 1(School of Chemical Engineering, Hunan Chemical Vocational Technology College, Zhuzhou 412000, China)
    2(School of Pharmaceutical and Bioengineering, Hunan Chemical Vocational Technology College, Zhuzhou 412000, China)

Received date: 2025-06-10

  Revised date: 2025-07-15

  Online published: 2025-12-15

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摘要

抗菌肽是一类具有显著抑菌活性的多肽类物质,具有稳定性好、不易产生耐药性和生物安全性高等优势,是传统抗生素的潜在替代品。目前,抗菌肽主要有生物提取法、化学合成法和基因工程表达法3种制备途径。抗菌肽微生物异源表达法属基因工程表达法的一种,作为一种新型制备方法,具有更高的经济性、便捷性和高效性,是抗菌肽规模化生产的重要策略和制备手段。其中,异源表达系统的选择和表达策略的优化是制约抗菌肽规模化生产的关键。该文在系统介绍抗菌肽微生物异源表达系统发展的基础上,对抗菌肽微生物异源表达策略优化进行了系统阐述,并对抗菌肽微生物异源表达优化策略进行展望,以期为抗菌肽的高效表达和规模化生产提供理论与实践参考。

关键词： 抗菌肽; 微生物异源表达; 真核表达系统; 原核表达系统; 表达策略

本文引用格式

李玉珍 , 肖怀秋 , 王斌 , 廖琼 , 刘畅宇 , 唐旭阳 . 抗菌肽微生物异源表达系统和表达策略优化研究进展[J]. 食品与发酵工业, 2025 , 51(22) : 411 -419 . DOI: 10.13995/j.cnki.11-1802/ts.043507

Abstract

Antimicrobial peptides (AMPs) are a class of polypeptides characterized by excellent antibacterial activity, remarkable stability, low propensity for inducing drug resistance and high biological safety, these attributes render them promising alternatives to conventional antibiotics.Presently, there is three production methods that employed for AMPs production, including bio-extraction, chemical synthesis, and gene engineering expression.Microbial heterologous expression is one of gene engineering expression methods, has garnered significant attention.As a novel preparation method, microbial heterologous expression offers distinct advantages in terms of economy, convenience, and efficiency, thereby emerging as a crucial strategy for the large-scale production of AMPs.The selection of heterologous expression systems and expression optimization strategies are pivotal factors influencing the successful large-scale production of AMPs.This paper systematically reviews the development of microbial heterologous expression systems for AMPs and further elaborates on the optimization strategies of microbial heterologous expression, providing a comprehensive overview on the current state of this field.Additionally, this review offers insights and prospects for future advancements in microbial heterologous expression.The objective is to provide both theoretical and practical guidelines for the efficient expression and large-scale production of AMPs.

Key words： antimicrobial peptides; microbial heterologous expression; eukaryotic expression system; prokaryotic expression system; expression strategies

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