研究报告

基于16S rRNA基因测序和Source Tracker模型分析生驼乳安全风险关键点

  • 薛宇飞 ,
  • 冀承昊 ,
  • 何静 ,
  • 吉日木图
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  • 1(内蒙古农业大学 食品科学与工程学院,内蒙古 呼和浩特,010018)
    2(中国-蒙古国高分子应用“一带一路”联合实验室,内蒙古 呼和浩特,010000)
    3(内蒙古中哈骆驼研究院,内蒙古 阿拉善盟,737300)
第一作者:硕士研究生(吉日木图教授为通信作者,E-mail:yeluotuo1999@vip.163.com)

收稿日期: 2024-09-21

  修回日期: 2025-04-06

  网络出版日期: 2025-12-25

基金资助

内蒙古自治区科技计划项目(2024KJHZ0001);内蒙古自治区骆驼现代农牧业产业技术体系(IMARS-13)

Analysis of safety risk key points of raw camel milk based on 16S rRNA gene sequencing and Source Tracker model

  • XUE Yufei ,
  • JI Chenghao ,
  • HE Jing ,
  • JI Rimutu
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  • 1(College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)
    2(China-Mongolia Joint Laboratory of Biopolymer Application “One Belt One Road”, Hohhot 010000, China)
    3(Inner Mongolia Zhongha Camel Research Institute, Alxa League 737300, China)

Received date: 2024-09-21

  Revised date: 2025-04-06

  Online published: 2025-12-25

摘要

驼乳是人类各类营养元素摄入的良好来源之一,富含的营养价值更易被吸收利用,对人体健康、疾病预防及治疗具有重要作用。但由于驼乳受到不卫生的环境条件、生产过程不规范或处理方法不当等影响,极易遭受微生物的污染,这不仅威胁到驼乳产品的品质与安全,也成为了制约驼乳产业持续健康发展的关键因素之一。因此,该研究通过16S rRNA扩增子测序技术对挤奶瓶、乳头夹、储奶罐、乳头、饮用水、饲料、粪便和驼舍空气样品中微生物多样性比较分析。采用Source Tracker分析模型对3个牧场中的微生物风险关键点分析。研究表明,A驼场生乳中乳球菌和金黄杆菌在属水平上的相对丰度较高,占比分别为52.37%和15.76 %;而在B驼场生乳在属水平上主要优势菌属为罗思氏菌,相对丰度占比高达83.64%;C驼场生乳微生物属占比较高的为罗思氏菌和不动杆菌,分别为64.85%和17.58%。利用Source Tracker模型对A、B和C三个驼场的挤奶瓶、乳头夹、储奶罐、乳头、饮用水、饲料、粪便和驼舍空气样品8个风险关键点进行分析,确定造成原料乳微生物污染的风险关键点为乳头、乳头夹、挤奶瓶和储奶罐。该研究结果可为生驼乳安全性的提高提供参考依据。

本文引用格式

薛宇飞 , 冀承昊 , 何静 , 吉日木图 . 基于16S rRNA基因测序和Source Tracker模型分析生驼乳安全风险关键点[J]. 食品与发酵工业, 2025 , 51(23) : 235 -243 . DOI: 10.13995/j.cnki.11-1802/ts.041106

Abstract

Camel milk is one of the good sources of human nutrition intake, which is rich in nutritional value and easier to be absorbed and utilized.It plays an important role in human health and the prevention and treatment of diseases.However, due to the impact of unsanitary environmental conditions, non-standard production processes, or improper treatment methods, camel milk is extremely susceptible to microbial pollution, which not only threatens the quality and safety of camel milk products but also becomes one of the key factors restricting the sustainable and healthy development of camel milk industry.Therefore, microbial diversity in milk bottles, nipple clips, milk storage cans, nipples, drinking water, feed, feces, and camel house air samples was analyzed by 16S rRNA amplification technology.The Source Tracker analysis model was used to analyze the key risk points of microorganisms in three pastures.Results showed that the relative abundances of Lactococcus and Chryseobacterium in the raw milk of camel farm A were higher at the genus level, accounting for 52.37% and 15.76% respectively.The dominant genus of raw milk in camel farm B was Rothia, with a relative abundance of 83.64%.The bacteria genera with high proportions in the raw milk of camel farm C were Rothia and Acinetobacter, which were 64.85% and 17.58%, respectively.The Source Tracker model was used to analyze eight risk key points, including milk bottles, nipple clips, milk storage cans, nipples, drinking water, feed, feces, and camel house air samples from camel farms A, B, and C.The key risk points of raw milk microbial contamination were identified as nipples, nipple clips, milk bottles, and milk storage cans.These results can provide a reference for improving the safety of raw camel milk.

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