为了进一步提升生物法琼脂脱硫的效果,促进琼脂工业的绿色转型,该研究尝试将安全性高的解脂耶氏酵母菌株应用于琼脂脱硫,并使用解脂耶氏酵母及巨大芽孢杆菌菌株通过连续转接的方式对琼脂进行发酵脱硫。结果表明,解脂耶氏酵母可以琼脂为唯一硫源生长而不以琼脂为碳源,在对不同形态的琼脂脱硫中,其对于条状琼脂品质的提升最为明显;使用解脂耶氏酵母采取连续转接的方式对琼脂的脱硫率高达96.19%,硫含量降低至0.04%,凝胶强度达到959.63 g/cm2,相比于原始琼脂提升了165.08%,脱硫后的琼脂品质接近于琼脂糖水平并可应用于凝胶电泳,说明解脂耶氏酵母是可以有效提升琼脂质量的菌株。采用巨大芽孢杆菌进行连续转接脱硫使得琼脂的硫含量达到0.13%,脱硫率达88.04%,凝胶强度达到1 067.78 g/cm2,提升了194.95%,有效改善了琼脂的质量。
To further enhance the effect of biological agar desulfation and promote the green transformation of the agar industry, this study attempted to utilize a safe strain of Yarrowia lipolytica for agar desulfation.Additionally, the study employed strains of Y.lipolytica and Priestia megaterium to ferment and desulfurize agar through continuous transfer.Results showed that Y.lipolytica could grow with agar as the only sulfur source and did not rely on agar as a carbon source.The quality of the strip agar was most significantly improved through the desulfation of different forms of agar.The desulfation rate of agar was as high as 96.19%, the sulfur content was reduced to 0.04%, and the gel strength reached 959.63 g/cm2, which was 165.08% higher than that of the original agar by continuous transfer using Y.lipolytica, and the quality of the desulfurized agar was close to the agarose level and could be applied to gel electrophoresis, indicating that the strain of Y.lipolytica could effectively improve the quality of agar.The sulfur content of agar reached 0.13%, the desulfation rate reached 88.04%, and the gel strength reached 1 067.78 g/cm2, which was increased by 194.95% by continuous transfer using P.megaterium, which effectively improved the quality of agar.
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