食品与发酵工业 >

2026 , Vol. 52 >Issue 1: 39 - 46

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041587

研究报告

短乳杆菌SLlac-18安全性评价及对高尿酸血症小鼠的益生作用

  • 李杰 ,
  • 李彦杰 ,
  • 史岩 ,
  • 罗进城 ,
  • 周健 ,
  • 孙雪微 ,
  • 王雪 ,
  • 宋丽新
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  • 1(佳木斯大学 公共卫生学院,黑龙江 佳木斯,154003)
    2(佳木斯大学,实验动物中心,黑龙江 佳木斯,154003)
    3(包头市疾病预防控制中心,内蒙古 包头,014030)
第一作者:硕士,实验师(宋丽新主管技师为通信作者,E-mail:songlixin1015@163.com)

收稿日期: 2024-11-13

  修回日期: 2025-04-30

  网络出版日期: 2026-01-22

基金资助

黑龙江省省属高等学校基本科研业务费科研项目(2022-KYYWF-0649);黑龙江省“双一流”学科协同创新成果项目(LJGXCG2023-089);内蒙古自治区包头市卫生健康科技计划项目(2023wsjkkj43);佳木斯大学东极团队项目(DJXSTD202404)

收起

Safety evaluation and probiotic effect of Lactobacillus brevis SLlac-18 on hyperuricemic mice

  • LI Jie ,
  • LI Yanjie ,
  • SHI Yan ,
  • LUO Jincheng ,
  • ZHOU Jian ,
  • SUN Xuewei ,
  • WANG Xue ,
  • SONG Lixin
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  • 1(School of Public Health, Jiamusi University, Jiamusi 154003, China)
    2(Laboratory Animal Center, Jiamusi University, Jiamusi 154003, China)
    3(Baotou Disease Prevention Control Center, Baotou 014030, China)

Received date: 2024-11-13

  Revised date: 2025-04-30

  Online published: 2026-01-22

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摘要

高尿酸血症是一种因尿酸代谢障碍而引发的代谢性疾病,而益生菌缓解高尿酸血症具有经济有效、毒副作用小、安全性相对较高等特点。该研究对前期分离自泡菜的1株具有潜在降尿酸功能的短乳杆菌(Lactobacillus brevis)SLlac-18开展安全性评价,并且通过构建高尿酸血症模型小鼠,探究短乳杆菌SLlac-18对高尿酸血症小鼠的益生作用。结果表明:短乳杆菌SLlac-18不具有溶血活性,小鼠灌胃该菌株后,其体重、脏器指数和血常规相比对照组并无显著差异,且肝脏和肾脏组织未出现明显病理变化;此外,短乳杆菌SLlac-18可将高尿酸血症小鼠血清尿酸水平和黄嘌呤氧化酶(xanthine oxidase, XOD)活力分别降低32.02%和80.77%(P<0.05),并可改善高尿酸血症小鼠肝肾组织损伤,使肝脏中XOD活力显著降低63.38%(P<0.05),使肾脏中的尿酸重吸收转运蛋白GLUT9表达量下降23.74%,而尿酸分泌转运蛋白NPT1表达量上升23.68%。由此表明,短乳杆菌SLlac-18具有良好的安全性,并能通过多种途径对高尿酸血症小鼠发挥益生作用,在高尿酸血症的预防与辅助治疗中具有应用潜力。

关键词： 短乳杆菌; 高尿酸血症; 尿酸; 益生菌; 安全性

本文引用格式

李杰 , 李彦杰 , 史岩 , 罗进城 , 周健 , 孙雪微 , 王雪 , 宋丽新 . 短乳杆菌SLlac-18安全性评价及对高尿酸血症小鼠的益生作用[J]. 食品与发酵工业, 2026 , 52(1) : 39 -46 . DOI: 10.13995/j.cnki.11-1802/ts.041587

Abstract

Hyperuricaemia is a metabolic disease characterized by uric acid metabolism disorder.Probiotics have the characteristics of being economical and effective with few toxic side effects and relatively high safety for alleviating hyperuricemia.In this study, we evaluated the safety of a previously isolated strain of Lactobacillus brevis SLlac-18 from a pickle with potential uric acid-lowering function and constructed a hyperuricemia mice model to investigate the probiotic effects of L.brevis SLlac-18 on hyperuricemic mice.The results showed that L.brevis SLlac-18 had no haemolytic activity, and there were no significant differences in body weight, organ indices, and blood routine indexes in mice gavaged with strain compared to the control group, and no significant pathological changes in liver and kidney tissue were observed.Additionally, compared with the model group, the expression levels of uric acid and xanthine oxidase (XOD) in serum were decreased to 32.02% and 80.77% respectively in the SLlac-18 group (P<0.01), liver and kidney tissue damage was improved by L.brevis SLlac-18, and the XOD activity was decreased to 63.38% (P<0.01), the expression of uric acid reabsorption transporter protein GLUT9 was reduced to 23.74% and the expression the uric acid secretion transporter protein NPT1 was increased to 23.68%.In conclusion, L.brevis SLlac-18 exhibits a favorable safety profile and exerts beneficial effects in hyperuricemic mice through multiple pathways, holding promise for the prevention and adjuvant therapy of hyperuricemia.

Key words： Lactobacillus brevis; hyperuricaemia; uric acid; probiotics; safety

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