食品与发酵工业 >

2026 , Vol. 52 >Issue 3: 223 - 229

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041782

研究报告

复合乳酸菌发酵红糖工艺优化及感官品质的研究

  • 王文鑫 ,
  • 王昊 ,
  • 段青丽 ,
  • 于有强 ,
  • 陈闪 ,
  • 郑骁阳 ,
  • 马喜山 ,
  • 宋姿樾 ,
  • 吴树波 ,
  • 杨丹 ,
  • 段盛林 ,
  • 刘士伟
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  • 1(中国食品发酵工业研究院有限公司,北京,100015)
    2(中国糖业酒类集团有限公司,北京,100032)
第一作者:本科,助理工程师(刘士伟高级工程师为通信作者,E-mail:ff_nns@163.com)

收稿日期: 2024-12-05

  修回日期: 2025-05-26

  网络出版日期: 2026-03-13

基金资助

广西重点研发计划项目(桂科AB25069469)

收起

Research on process optimization and sensory quality of brown sugar fermented by compound lactic acid bacteria

  • WANG Wenxin ,
  • WANG Hao ,
  • DUAN Qingli ,
  • YU Youqiang ,
  • CHEN Shan ,
  • ZHENG Xiaoyang ,
  • MA Xishan ,
  • SONG Ziyue ,
  • WU Shubo ,
  • YANG Dan ,
  • DUAN Shenglin ,
  • LIU Shiwei
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  • 1(China National Research Institute of Food & Fermentation Industries Co. Ltd., Beijing 100015, China)
    2(China National Sugar and Alcohol Group Co. Ltd., Beijing 100032, China)

Received date: 2024-12-05

  Revised date: 2025-05-26

  Online published: 2026-03-13

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摘要

采用乳酸菌对红糖进行生物加工是提升产品功能价值、改善产品感官特性、赋予产品高附加值的可行途径。该研究采用鼠李糖乳酪杆菌、嗜酸乳杆菌组合的混菌发酵模式,对红糖原料进行液态发酵。通过对比不同发酵工艺参数对发酵红糖液pH及DPPH自由基清除率的影响,优化发酵工艺。通过电子感官与人体感官相结合的方式,对比不同稀释度下发酵红糖的感官品质。结果表明,红糖原料发酵的最优条件为发酵时间35 h、发酵温度36 ℃、接菌量0.2 g/L,以此条件制备的发酵红糖对DPPH自由基清除能力达到80.19%。同时,不同稀释度的红糖发酵液、红糖原液之间电子感官数据存在显著差异。红糖发酵液稀释度为15%时感官品质最佳。该研究为开发功能性红糖饮品奠定了理论基础。

关键词： 发酵红糖; 工艺优化; 抗氧化活性; 电子感官; 人工感官评价

本文引用格式

王文鑫 , 王昊 , 段青丽 , 于有强 , 陈闪 , 郑骁阳 , 马喜山 , 宋姿樾 , 吴树波 , 杨丹 , 段盛林 , 刘士伟 . 复合乳酸菌发酵红糖工艺优化及感官品质的研究[J]. 食品与发酵工业, 2026 , 52(3) : 223 -229 . DOI: 10.13995/j.cnki.11-1802/ts.041782

Abstract

The bioprocessing of brown sugar using lactic acid bacteria is a viable approach to enhance the functional values of products, improve their sensory characteristics, and endow them with high added value.In this study, a mixed fermentation method using Lacticaseibacillus rhamnosus and Lactobacillus acidophilus was used to carry out the liquid fermentation of brown sugar.The fermentation process was optimized by comparing the effects of different fermentation process parameters on the pH value of the fermented brown sugar solution and its DPPH free radical scavenging rate.Evaluation of the sensory quality of fermented brown sugar with different dilutions was conducted by combining electronic and human senses.The results showed that the optimal fermentation conditions were determined to be fermentation at 36 ℃ for 35 h with a 0.2 g/L of bacterial inoculation.Under the optimal fermentation conditions, the DPPH free radical scavenging activity of fermentation brown sugar was up to 80.19%.Additionally, the results of electronic sensory showed significantly different flavors between fermented and unfermented brown sugar solutions at different dilution levels.And the brown sugar fermentation solution with a 15% dilution exhibited the best organoleptic quality.In conclusions, this study would lay a theoretical foundation for the development of functional brown sugar beverages.

Key words： fermented brown sugar; process optimization; antioxidant activity; electronic senses; artificial sensory evaluation

参考文献

[1] JAFFÉ W R.Health effects of non-centrifugal sugar (NCS):A review[J].Sugar Tech, 2012, 14(2):87-94.
[2] 徐灵均, 袁义明, 冯爱国, 等.传统红糖与精制赤砂糖理化性质比较[J].食品科学, 2018, 39(7):125-129.
XU L J, YUAN Y M, FENG A G, et al.Comparative studies on physical and chemical properties of traditional brown sugar and refined brown granulated sugar[J].Food Science, 2018, 39(7):125-129.
[3] TAKARA K, KINJYO A, MATSUI D, et al.Antioxidative phenolic compounds from non-sugar fraction in Kokuto, non-centrifugal cane sugar[J].Journal of the Agricultural Chemical Society of Japan, 2000, 74(8):885-890.
[4] CALABIA B P, TOKIWA Y.Production of D-lactic acid from sugarcane molasses, sugarcane juice and sugar beet juice by Lactobacillus delbrueckii[J].Biotechnology Letters, 2007, 29(9):1329-1332.
[5] MOKOENA M P.Lactic acid bacteria and their bacteriocins:Classification, biosynthesis and applications against uropathogens:A mini-review[J].Molecules, 2017, 22(8):1255.
[6] SENGUN I Y, KILIC G, CHAROENYINGCHAROEN P, et al.Investigation of the microbiota associated with traditionally produced fruit vinegars with focus on acetic acid bacteria and lactic acid bacteria[J].Food Bioscience, 2022, 47:101636.
[7] YEBOAH P J, WIJEMANNA N D, EDDIN A S, et al.Lactic acid bacteria:Review on the potential delivery system as an effective probiotic[J].Current Issues and Advances in the Dairy Industry.London:IntechOpen, 2023
[8] 王超, 刘青飞, 黄红艳.乳酸菌发酵香蕉、石斛复合汁的工艺条件优化及挥发性风味物质分析[J].饮料工业, 2024, 27(6):5-10.
WANG C, LIU Q F, HUANG H Y.Optimization of fermentation process of banana-Dendrobium officinale juice by lactic acid bacteria and analysis of volatile flavor substances[J].Beverage Industry, 2024, 27(6):5-10.
[9] 马晓娟, 谢有发, 倪彩新, 等.植物乳杆菌P9发酵对枸杞浆品质的影响[J].食品与发酵工业, 2023, 49(12):229-234.
MA X J, XIE Y F, NI C X, et al.Effect of Lactobacillus plantarum P9 fermentation on the quality of Lycium barbarum pulp[J].Food and Fermentation Industries, 2023, 49(12):229-234.
[10] 潘胜国, 王群, 刘制杰, 等.乳酸菌发酵对枇杷汁体外降血糖、降血脂活性的影响[J].食品工业科技, 2025, 46(7):161-168.
PAN S G, WANG Q, LIU Z J, et al.Effect of lactic acid bacteria fermentation on the hypoglycemic and hypolipidemic activities of loquat juice in vitro[J].Science and Technology of Food Industry, 2025, 46(7):161-168.
[11] 冯丹丹, 胡萍, 许浩翔, 等.乳酸菌发酵刺梨汁体外降血糖、降血脂活性研究[J].食品与发酵工业, 2022, 48(8):212-219.
FENG D D, HU P, XU H X, et al.Hypoglycemic and hypolipidemic activities of Rosa roxburghii juice fermented by lactic acid bacteria in vitro[J].Food and Fermentation Industries, 2022, 48(8):212-219.
[12] 高小朋, 何猛超, 许可, 等.工业微生物发酵过程中pH调控研究进展[J].中国生物工程杂志, 2020, 40(6):93-99.
GAO X P, HE M C, XU K, et al.Research progress on pH regulation in the process of industrial microbial fermentation[J].China Biotechnology, 2020, 40(6):93-99.
[13] 王储炎, 张继刚, 杨柳青, 等.3种乳酸菌发酵对蓝莓多酚、原花青素含量及抗氧化活性的影响[J].食品科学, 2020, 41(24):87-94.
WANG C Y, ZHANG J G, YANG L Q, et al.Comparative effects of fermentation with three species of lactic acid bacteria on polyphenol and proanthocyanidin contents and antioxidant activity of blueberry fruit[J].Food Science, 2020, 41(24):87-94.
[14] PARK J B, LIM S H, SIM H S, et al.Changes in antioxidant activities and volatile compounds of mixed berry juice through fermentation by lactic acid bacteria[J].Food Science and Biotechnology, 2017, 26(2):441-446.
[15] VALERO-CASES E, CERDÁ-BERNAD D, PASTOR J J, et al.Non-dairy fermented beverages as potential carriers to ensure probiotics, prebiotics, and bioactive compounds arrival to the gut and their health benefits[J].Nutrients, 2020, 12(6):1666.
[16] 张郁松. 纯种发酵红皮萝卜泡菜工艺[J].食品工业, 2023, 44(2):38-40.
ZHANG Y S.Pickling technology of pure breed fermented radish with red skin[J].The Food Industry, 2023, 44(2):38-40.
[17] 周灿, 齐成媚, 杨芳, 等.生姜酵素发酵工艺优化及抗氧化活性研究[J].中国酿造, 2024, 43(10):82-87.
ZHOU C, QI C M, YANG F, et al.Optimization of fermentation process and antioxidant activity of ginger Jiaosu[J].China Brewing, 2024, 43(10):82-87.
[18] 王佳, 黑果酵素的制备及抗氧化活性研究[D].青岛:青岛农业大学,2019.
WANG J.Preparation and antioxidant activity of black chokeberry enzyme[D].Qingdao:Qingdao Agricultural University,2019.
[19] 陈林林, 王玲, 郑凤鸣, 等.发酵紫苏粕制备抗氧化肽的工艺优化及抗氧化性[J].食品研究与开发, 2023, 44(18):116-124.
CHEN L L, WANG L, ZHENG F M, et al.Process optimization and activity of antioxidant peptides prepared from fermented perilla meal[J].Food Research and Development, 2023, 44(18):116-124.
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