食品与发酵工业 >

2026 , Vol. 52 >Issue 4: 40 - 47

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043612

研究报告

不同冷藏条件对两种虾肌原纤维蛋白的影响

  • 雷淑君 ,
  • 胡敏 ,
  • 丁虞妍 ,
  • 张宾 ,
  • 房传栋 ,
  • 涂传海
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  • 1(浙江海洋大学 食品与药学学院,浙江 舟山,316022)
    2(浙江省海产品健康危害因素关键技术研究重点实验室,浙江 舟山,316000)
    3(浙江海洋大学 中意比萨海洋研究生学院,浙江 舟山,316022)
第一作者:硕士研究生(房传栋讲师和涂传海副教授为共同通信作者,E-mail:fang_cd@126.com;tu2021@zjou.edu.cn)

收稿日期: 2025-06-16

  修回日期: 2025-07-04

  网络出版日期: 2026-03-17

基金资助

国家自然科学基金项目(U23A20263);浙江省自然科学基金项目(LRG25C200001)

收起

Effects of different refrigerated storage conditions on myofibrillar proteins from two shrimp species

  • LEI Shujun ,
  • HU Min ,
  • DING Yuyan ,
  • ZHANG Bin ,
  • FANG Chuandong ,
  • TU Chuanhai
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  • 1(College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China)
    2(Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan 316000, China)
    3(Pisa Marine Graduate School of Zhejiang Ocean University, Zhoushan 316022, China)

Received date: 2025-06-16

  Revised date: 2025-07-04

  Online published: 2026-03-17

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摘要

该研究以南美白对虾(Litopenaeus vannamei,LV)和哈氏仿对虾(Parapenaeopsis hardwickii,PH)的肌原纤维蛋白(myofibrillar proteins,MPs)为对象,对比分析4 ℃冷藏贮存与模拟运输贮存0~4 d对肌原纤维蛋白理化性质的影响。通过测定总巯基含量、羰基含量、表面疏水性、粒径、二、三级结构及SDS-PAGE等指标,发现2种虾的MPs变性程度存在显著差异(P<0.05)。结果表明,在4 d的冷藏过程中,LV和PH的表面疏水性和羰基含量增加,总巯基含量下降,粒径、C—H峰强度和内源性荧光强度均呈现出先增加后减少的趋势;SDS-PAGE显示模拟运输贮存第4天时出现了肌动蛋白条带颜色变浅和新条带产生,表明MPs发生了分解和聚集。总体而言,模拟运输贮存条件对MPs的破坏性高于4 ℃冷藏处理,LV的MPs稳定性显著高于PH(P<0.05)。该研究旨在阐明不同贮存环境对2种虾MPs的分子损伤程度,为LV和PH差异化冷藏保鲜技术开发提供理论支持。

关键词： 肌原纤维蛋白; 南美白对虾; 哈氏仿对虾; 模拟运输贮存; 二级分子结构

本文引用格式

雷淑君 , 胡敏 , 丁虞妍 , 张宾 , 房传栋 , 涂传海 . 不同冷藏条件对两种虾肌原纤维蛋白的影响[J]. 食品与发酵工业, 2026 , 52(4) : 40 -47 . DOI: 10.13995/j.cnki.11-1802/ts.043612

Abstract

This study investigated the comparative effects of refrigerated storage at 4 ℃ and simulated transport storage conditions (0-4 days) on the physicochemical properties of myofibrillar proteins (MPs) isolated from Litopenaeus vannamei (LV) and Parapenaeopsis hardwickii (PH).Significant differences (P<0.05) in the denaturation extent of MPs between the two shrimp species were observed through analysis of total sulfhydryl content, carbonyl content, surface hydrophobicity, particle size, secondary and tertiary structures, and SDS-PAGE.Results demonstrated that during the 4-day storage period, both LV and PH MPs exhibited significant increases in surface hydrophobicity and carbonyl content, accompanied by a decrease in total sulfhydryl content.Particle size, C—H peak intensity, and intrinsic fluorescence intensity displayed a consistent trend of initial increase followed by subsequent decrease.SDS-PAGE analysis revealed the fading of actin bands and the emergence of new bands by day 4 under simulated transport conditions, indicating MP degradation and aggregation.Overall, simulated transport storage conditions exerted more deleterious effects on MPs than those at 4 ℃ refrigeration, with LV demonstrating significantly higher MPs stability than PH (P<0.05).This study elucidates the extent of molecular damage in MPs from both shrimp species under distinct storage environments, providing theoretical support for developing tailored cold storage technologies for LV and PH.

Key words： myofibrillar proteins; Litopenaeus vannamei; Parapenaeopsis hardwickii; simulated transportation storage; secondary molecular structure

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