食品与发酵工业 >

2026 , Vol. 52 >Issue 2: 19 - 27

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042361

研究报告

过表达谷胱甘肽过氧化物酶基因对草菇抗氧化性能和生产能力的影响

  • 孙建霞 ,
  • 王永慧 ,
  • 王瀚强 ,
  • 朱嘉宁 ,
  • 贠建民 ,
  • 赵风云
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  • (甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070)
第一作者:硕士研究生(赵风云教授为通信作者,E-mail:fyzhao@gsau.edu.cn)

收稿日期: 2025-02-13

  修回日期: 2025-05-21

  网络出版日期: 2026-03-18

基金资助

国家自然科学基金项目(32060708)

收起

Effects of overexpression of glutathione peroxidase gene on antioxidant properties and productivity in Volvariella volvacea

  • SUN Jianxia ,
  • WANG Yonghui ,
  • WANG Hanqiang ,
  • ZHU Jianing ,
  • YUN Jianmin ,
  • ZHAO Fengyun
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2025-02-13

  Revised date: 2025-05-21

  Online published: 2026-03-18

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摘要

活性氧(reactive oxygen species,ROS)积累是影响食用菌产量的重要因素,谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)是一种清除ROS的重要抗氧化酶。该文以课题组前期获得的草菇gpx基因过表达转化子OE-V3、OE-V4、OE-V16和OE-V21为实验菌株,对转化子的抗氧化性能、ROS含量、基质降解酶活力、生产性状等指标进行测定,结果表明,gpx基因过表达提高了草菇的Cu/Zn-sod、Mn-sod2、cat1、cat2、gpx和gr基因的相对表达量,增加了GPX、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽还原酶(glutathione reductase,GR)的活力;各菌株的ROS含量随着gpx基因表达量的升高而降低,还原型谷胱甘肽(glutathione,GSH)、氧化型谷胱甘肽(glutathione disulfide,GSSG)含量及GSH/GSSG值显著提高;gpx基因过表达也使草菇抵御氧化胁迫的能力显著增强,有效提高了草菇的基质降解酶活力,使其菌丝生长速度加快,生产周期缩短、生物学效率显著提高。该研究表明了gpx基因过表达是提高草菇子实体产量的一种有效策略。

关键词： 草菇; 谷胱甘肽过氧化物酶基因; 抗氧化能力; ROS含量; 基质降解酶; 生物学效率

本文引用格式

孙建霞 , 王永慧 , 王瀚强 , 朱嘉宁 , 贠建民 , 赵风云 . 过表达谷胱甘肽过氧化物酶基因对草菇抗氧化性能和生产能力的影响[J]. 食品与发酵工业, 2026 , 52(2) : 19 -27 . DOI: 10.13995/j.cnki.11-1802/ts.042361

Abstract

Reactive oxygen species (ROS) accumulation is an important factor affecting the yield of edible fungi, glutathione peroxidase (GPX) is an important antioxidant enzyme for scavenging ROS.The article used the previously obtained overexpression transformants of the gpx gene from the Volvariella volvacea, namely OE-V3, OE-V4, OE-V16, and OE-V21, as experimental strains to measure indicators, such as the antioxidant performance of the transformants, ROS content, substrate degrading enzyme activity, and productive traits.The results showed that gpx gene overexpression increased the relative expression of Cu/Zn-sod, Mn-sod2, cat1, cat2, gpx and gr genes, and the enzyme activities of GPX, superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) in V.volvacea.The ROS content of each strain decreased with the increase of gpx gene expression, the glutathione (GSH), glutathione disulfide (GSSG) contents and GSH/GSSG values were significantly increased, gpx gene overexpression also significantly enhanced the ability of V.volvacea to withstand oxidative stress, effectively increased the activity of matrix degrading enzyme of V.volvacea, and accelerated the growth of their mycelium, shortened the production cycle, and significantly improved the biological efficiency.This study demonstrated that gpx gene overexpression is an effective strategy to improve the production of V.volvacea.

Key words： Volvariella volvacea; glutathione peroxidase gene; antioxidant capacity; ROS content; matrix degrading enzyme; biological efficiency

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