食品与发酵工业 >

2026 , Vol. 52 >Issue 2: 145 - 155

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042870

研究报告

基于超高效液相色谱-四极杆飞行时间串联质谱技术、网络药理学和动物实验探究人参沙棘复合物抗疲劳作用机制

  • 黄海林 ,
  • 喻勤 ,
  • 刘文利 ,
  • 黄子进 ,
  • 丰时运 ,
  • 伍蓥 ,
  • 高业成 ,
  • 谢果
展开
  • 1(广州中医药大学中药学院,广东 广州,510000)
    2(完美(广东)日用品有限公司,广东 中山,528451)
    3(电子科技大学中山学院,广东 中山,528402)
第一作者:硕士研究生(高业成中级食品工程师和谢果副教授为共同通信作者,E-mail:gaoyc@perfect99.com;xieguo@zsc.edu.cn

收稿日期: 2025-03-28

  修回日期: 2025-06-06

  网络出版日期: 2026-03-18

基金资助

国家自然科学基金项目(32101903);中山市中医药传承创新发展科研专项(2024B3065)

收起

Exploring anti-fatigue mechanisms of ginseng sea buckthorn complex based on ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry, network pharmacology, and animal experiments

  • HUANG Hailin ,
  • YU Qin ,
  • LIU Wenli ,
  • HUANG Zijin ,
  • FENG Shiyun ,
  • WU Ying ,
  • GAO Yecheng ,
  • XIE Guo
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  • 1(College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510000, China)
    2(Perfect (Guangdong) Commodity Co., Ltd., Zhongshan 528451, China)
    3(Zhongshan Institute, University of Electronic Science and Technology of China, Zhongshan 528402, China)

Received date: 2025-03-28

  Revised date: 2025-06-06

  Online published: 2026-03-18

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摘要

该研究运用超高效液相色谱-四极杆飞行时间串联质谱技术(ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry,UPLC-QTOF-MS)分析人参沙棘复合物(ginseng sea buckthorn complex,GSC)活性成分并借助网络药理学、分子对接及动物实验探讨其抗疲劳作用机制。采用UPLC-QTOF-MS技术鉴定GSC和人参提取物(ginseng extract,GE)中的人参皂苷种类及相对含量;利用网络药理学分子对接预测GSC抗疲劳潜在作用通路;通过评估GSC成分与关键靶点的结合特性;设计动物实验验证GSC抗疲劳功效及机制。结果表明,GE中含有23种人参皂苷,GSC中共含有17种人参皂苷,GSC相比于GE稀有皂苷含量得到提升。网络药理学预测GSC可能通过作用于磷脂酰肌醇3′-激酶(phosphatidylinositol 3′-kinase,PI3K)-Akt等信号通路,影响能量代谢与代谢物废物清除起抗疲劳作用。分子对接结果显示,GSC中成分与关键靶点结果特性良好,结合能均低于-7.00 kcal/mol。动物实验表明,GSC改善了疲劳小鼠的能量代谢与代谢废物清除速率,使小鼠负重力竭游泳时间由105.4 s延长至395.9 s(P<0.001),肌糖原水平提高至32.30 mg/g(P<0.001),分别降低血乳酸曲线下面积至111.30 mmol/L(P<0.05)、尿素水平至7.52 mmol/L(P<0.05),还能通过调节肠道菌群起到抗疲劳作用。综上,GSC可能通过调节PI3K-Akt等信号通路和肠道菌群影响机体能量代谢和代谢废物清除发挥抗疲劳作用。

关键词： 人参沙棘复合物; 抗疲劳; 网络药理学; 分子对接; 能量代谢; 肠道菌群

本文引用格式

黄海林 , 喻勤 , 刘文利 , 黄子进 , 丰时运 , 伍蓥 , 高业成 , 谢果 . 基于超高效液相色谱-四极杆飞行时间串联质谱技术、网络药理学和动物实验探究人参沙棘复合物抗疲劳作用机制[J]. 食品与发酵工业, 2026 , 52(2) : 145 -155 . DOI: 10.13995/j.cnki.11-1802/ts.042870

Abstract

This study applied ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS) to analyze the active components of ginseng sea buckthorn complex (GSC).Network pharmacology, molecular docking, and animal experiments were employed to explore its anti-fatigue mechanism.UPLC-QTOF-MS was used to identify the types and relative contents of ginsenosides in GSC and ginseng extract (GE).Network pharmacology and molecular docking were utilized to predict the potential anti-fatigue pathways of GSC and evaluate the binding characteristics of GSC components with key targets.Animal experiments were designed to verify the anti-fatigue effect and mechanism of GSC.The results showed that GE contained 23 types of ginsenosides, while GSC contained 17 types, with an increased content of rare ginsenosides in GSC compared to GE.Network pharmacology predicted that GSC might act on signaling pathways like the phosphatidylinositol 3′-kinase (PI3K)-Akt, affecting energy metabolism and metabolite waste clearance to exert anti-fatigue effects.Molecular docking indicated that GSC components had good binding properties with key targets, with binding energies all below -7.00 kcal/mol.Animal experiments revealed that GSC improved energy metabolism and metabolic waste clearance in fatigued mice, extending the negative gravity exhaustion swimming time from 105.4 s to 395.9 s (P<0.001), increasing the muscle glycogen level to 32.30 mg/g (P<0.001), reducing the area under the blood lactic acid curve to 111.30 mmol/L (P<0.05) and the urea level to 7.52 mmol/L (P<0.05), and also exerting anti-fatigue effects by regulating gut microbiota.In conclusion, GSC may exert anti-fatigue effects by regulating signaling pathways like PI3K-Akt and gut microbiota, influencing energy metabolism and metabolic waste clearance.

Key words： ginseng sea buckthorn complex; anti-fatigue; network pharmacology; molecular docking; energy metabolism; intestinal flora

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