食品与发酵工业 >

2026 , Vol. 52 >Issue 5: 139 - 147

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042794

研究报告

黄芪多糖对嗜酸乳杆菌CICC 22162冷冻干燥保护效果的研究

  • 杜建明 ,
  • 张珍 ,
  • 尚琪 ,
  • 王学瑞 ,
  • 史建莉 ,
  • 宋乐 ,
  • 谢鹏 ,
  • 蔡毓涵
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  • (甘肃农业大学 食品科学与工程学院,甘肃 兰州,730000)
第一作者:硕士研究生(张珍教授为通信作者,E-mail:zhangzhen@gsau.edu.cn)

收稿日期: 2025-03-21

  修回日期: 2025-07-03

  网络出版日期: 2026-03-25

基金资助

甘肃省农业农村厅科技支撑项目(KJZC-2025-27);中国乳业协会利乐专项基金(CDIAKCJJ-TP-2024-008);甘肃省产业支撑项目(2023CYZC-43);甘肃省现代寒旱特色农业药产业技术体系(GSARS06-06)

收起

Cryoprotective effects of astragalus polysaccharides on freeze-drying survival of Lactobacillus acidophilus CICC 22162

  • DU Jianming ,
  • ZHANG Zhen ,
  • SHANG Qi ,
  • WANG Xuerui ,
  • SHI Jianli ,
  • SONG Le ,
  • XIE Peng ,
  • CAI Yuhan
Expand
  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730000, China)

Received date: 2025-03-21

  Revised date: 2025-07-03

  Online published: 2026-03-25

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摘要

黄芪多糖具有多种生理活性功能,其高效利用具有重要意义。该研究以提取纯化的黄芪多糖为原料,并与其他冻干保护剂复配,测定其对嗜酸乳杆菌CICC 22162冷冻干燥的保护效果。结果表明,黄芪多糖组分为64.38%葡萄糖、19.81%半乳糖和15.81%阿拉伯糖,复合保护剂使嗜酸乳杆菌存活率提升至(85.02±1.25)%。扫描电镜显示菌体形态更均匀,相较于传统保护剂,复合保护剂的碱性磷酸酶活性(3 450.61 U/g)降低15.5%,乳酸脱氢酶泄漏减少38.7%,溶菌酶敏感性实验中活菌数降幅减少20.6%,NaCl胁迫下膜损伤降低37.2%,组蛋白外泄量减少68%。证明黄芪多糖能更好地维持细胞壁和细胞膜的完整性,减少细胞内容物的泄漏,抵抗外界不利的环境变化。在模拟胃肠消化中菌株存活率达81.3%,较传统保护剂提升33.9%,4 ℃贮藏120 d后活菌数仍有9.18 lg CFU/mL,制备的冻干菌粉兼具高存活率、强胃肠耐受性和稳定性,研究结果为益生菌制剂提供了创新保护方案,在功能性食品和肠道微生态调节领域具有重要应用价值。

关键词： 黄芪多糖; 冷冻干燥保护剂; 嗜酸乳杆菌; 存活率; 细胞完整性; 稳定性

本文引用格式

杜建明 , 张珍 , 尚琪 , 王学瑞 , 史建莉 , 宋乐 , 谢鹏 , 蔡毓涵 . 黄芪多糖对嗜酸乳杆菌CICC 22162冷冻干燥保护效果的研究[J]. 食品与发酵工业, 2026 , 52(5) : 139 -147 . DOI: 10.13995/j.cnki.11-1802/ts.042794

Abstract

Astragalus polysaccharide (APS) exhibits multiple physiological activities, making its efficient development and utilization highly significant. This study investigated the cryoprotective effects of purified APS (composed of 64.38% glucose, 19.81% galactose, and 15.81% arabinose) in combination with other cryoprotectants on Lactobacillus acidophilus CICC 22162 during freeze-drying.Results showed that the APS-based cryoprotectant significantly improved bacterial survival to (85.02±1.25)%.Scanning electron microscopy revealed more uniform cellular morphology, compared with conventional cryoprotectants, the APS-based formulation also resulted in a 15.5% lower than conventional protectants, lactate dehydrogenase leakage decreased by 38.7%, viability reduction in lysozyme sensitivity tests was 20.6% lower, NaCl-induced membrane damage was reduced by 37.2%, and histone phosphorylation leakage decreased by 68%.These findings proved APS better maintains cell wall/membrane integrity, reduced intracellular content leakage, and enhanced resistance to environmental stresses.The formulation achieved 81.3% survival in simulated gastrointestinal digestion (33.9% improvement over conventional protectants) and maintained 9.18 lg CFU/mL viability after 120 days at 4 ℃.The APS-based cryoprotectant enables production of freeze-dried probiotics with high viability, excellent gastrointestinal tolerance, and superior stability, representing an innovative protection strategy with significant application value in functional foods and gut microbiota regulation.

Key words： astragalus polysaccharides; freeze drying protectant; Lactobacillus acidophilus; survival rate; cell integrity; stability

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