食品与发酵工业 >

2026 , Vol. 52 >Issue 5: 229 - 235

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042908

研究报告

马铃薯乳酸菌发酵饮料及高纤粉的代谢产物分析及通路解析

  • 朱莉莉 ,
  • 孙红男 ,
  • 马梦梅 ,
  • 木泰华
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  • (中国农业科学院农产品加工研究所,北京,100193)
第一作者:博士,助理研究员(孙红男研究员和马梦梅副研究员为共同通信作者,E-mail:sunhongnan@caas.cn;meimei881020@163.com)

收稿日期: 2025-04-17

  修回日期: 2025-07-04

  网络出版日期: 2026-03-25

基金资助

新疆自治区重点研发计划(厅厅联动)项目(2022B02044-3-1)

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Metabolites and pathway analysis of potato juice and high fiber powder prepared by lactic acid bacteria fermentation

  • ZHU Lili ,
  • SUN Hongnan ,
  • MA Mengmei ,
  • MU Taihua
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  • (Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Received date: 2025-04-17

  Revised date: 2025-07-04

  Online published: 2026-03-25

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摘要

该研究采用鼠李糖乳酪杆菌和戊糖片球菌复配对马铃薯进行发酵,制备马铃薯乳酸菌发酵饮料和高纤粉,通过超高效液相色谱-四极杆-飞行时间质谱系统分析了其代谢产物及相关代谢通路。结果表明,在马铃薯乳酸菌发酵饮料和高纤粉中分别鉴定出1 593和1 344种代谢物,基于变量投影重要度(variable importance in projection,VIP)≥1和P<0.05进一步确定了18种和19种关键差异代谢物。通过KEGG(Kyoto Encyclopedia of Genes and Genomes)通路富集分析发现,甘油磷脂代谢、精氨酸生物合成是马铃薯乳酸菌发酵饮料和高纤粉中共同存在的显著富集代谢通路,此外,烟酸/烟酰胺代谢、淀粉/蔗糖代谢分别是马铃薯乳酸菌发酵饮料和高纤粉中的差异性代谢通路;这些代谢通路与马铃薯中氨基酸代谢、磷脂胆碱生物合成、糖类分解代谢以及烟酰胺合成与代谢密切相关。该研究结果可为基于乳酸菌发酵技术开发马铃薯饮料和高纤粉产品提供理论依据和数据支持,对功能性食品的研发具有重要的参考价值。

关键词： 马铃薯; 乳酸菌; 发酵; 代谢产物; 代谢通路

本文引用格式

朱莉莉 , 孙红男 , 马梦梅 , 木泰华 . 马铃薯乳酸菌发酵饮料及高纤粉的代谢产物分析及通路解析[J]. 食品与发酵工业, 2026 , 52(5) : 229 -235 . DOI: 10.13995/j.cnki.11-1802/ts.042908

Abstract

The Lacticaseibacillus rhamnosus and Pediococcus pentosaceus co-fermentation were used to prepare potato fermented juice and high-fiber powder in this study, and the metabolites and related metabolic pathways were analyzed using an ultra-high-performance liquid chromatography-quadrupole-time of flight mass spectrometry.Results showed that 1 593 and 1 344 metabolites were identified in potato juice and high-fiber powder fermented by lactic acid bacteria, respectively, and 18 and 19 key differential metabolites were further screened based on variable importance in projection (VIP) ≥1 and P<0.05, respectively.The results of KEGG pathway enrichment analysis showed that glycerophospholipid metabolism and arginine biosynthesis were significant enriched metabolic pathways in potato fermented juice and high-fiber powder, while nicotinate/nicotinamide metabolism and starch/sucrose metabolism were the differential metabolic pathways in the potato fermented juice and high-fiber powder, respectively.These metabolic pathways were closely related to the potato amino acids metabolism, biosynthesis of phosphatidylcholine, carbohydrate catabolism, and nicotinamide synthesis and metabolism.The above results could provide a reference and data support for the development of potato juice and high-fiber powder products based on lactic acid bacteria fermentation technology, and have important reference value for the research and development of functional foods.

Key words： potato; lactic acid bacteria; fermentation; metabolites; metabolites pathway

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