研究报告

乳酸菌发酵百香果豆清饮料的动态品质分析:营养价值、抗氧化活性和感官风味

  • 胡晓婷 ,
  • 黄建芳 ,
  • 文明 ,
  • 陈浩 ,
  • 赵聃 ,
  • 张胤 ,
  • 苏伟明 ,
  • 谢乐 ,
  • 周小虎 ,
  • 周晓洁
展开
  • 1(邵阳学院 食品与化学工程学院,豆制品加工与安全控制湖南省重点实验室,湘味特色食品绿色加工与装备湖南省工程研究中心,湖南 邵阳,422000)
    2(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东 湛江,524000)
    3(湖南君益福食品有限公司,湖南 邵阳,422000)
    4(湖南乡乡嘴食品有限公司,湖南 邵阳,422000)
第一作者:硕士研究生(文明讲师和陈浩副教授为共同通信作者,E-mail:wenm8906@163.com;spgc13@163.com)

收稿日期: 2025-05-07

  修回日期: 2025-07-04

  网络出版日期: 2026-03-25

基金资助

邵阳学院研究生科研创新项目资助(CX2024SY018);邵阳学院大学生创新创业训练计划资助项目(S202410547005);邵阳市科技计划项目(2024GZ1001,2024PT6093,2024PT6091);湖南省自然科学基金资助项目(2023JJ50256,2025JJ70243,2023JJ50260);洞庭实验室专项经费项目(2024-DTKF-009);湖南省科技创新计划资助(2019TP1028,2020TP2002);湖南省教育厅科学研究项目(24C0397)

Dynamic quality analysis of lactic acid bacteria-fermented passion fruit soy whey drink:Nutritional value, antioxidant activity, and sensory-flavor characteristics

  • HU Xiaoting ,
  • HUANG Jianfang ,
  • WEN Ming ,
  • CHEN Hao ,
  • ZHAO Dan ,
  • ZHANG Yin ,
  • SU Weiming ,
  • XIE Le ,
  • ZHOU Xiaohu ,
  • ZHOU Xiaojie
Expand
  • 1(College of Food Science and Chemical Engineering, Shaoyang University, Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Hunan Engineering Research Center of Green Processing and Equipment of Hunan-style Food, Shaoyang 422000, China)
    2(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524000, China)
    3(Hunan Junyifu Food Co. Ltd., Shaoyang 422000, China)
    4(Hunan Xiangxiangzui Food Co. Ltd., Shaoyang 422000, China)

Received date: 2025-05-07

  Revised date: 2025-07-04

  Online published: 2026-03-25

摘要

为增加豆制品副产物-豆清液利用率,该研究以豆清液为原料复配百香果,并使用乳酸菌进行共发酵,探索发酵期间(0、6、12、18、24 h)理化指标、活菌数、营养成分、抗氧化活性及风味物质的变化规律。结果表明,乳酸菌百香果豆清饮料发酵18 h活菌数、抗氧化活性达到最高值,pH、总酸、可溶性固形物、总酚、总黄酮均显著上升后趋于平缓,而相较于纯豆清饮料,乳酸菌百香果豆清饮料中的大豆异黄酮葡萄糖苷型转化为苷元型的速率变慢。5个发酵阶段挥发性风味物质共检测到84种风味物质,以酯类和醇类为主导香气,18 h风味物质含量达到最高值1 333.30 μg/kg,偏最小二乘判别分析和气味活性值分析确定芳樟醇、乙酸己酯、月桂烯、冰醋酸为该饮料整体香气轮廓的关键标志化合物。该研究为豆制品副产物-豆清液再利用提供理论依据和参考。

本文引用格式

胡晓婷 , 黄建芳 , 文明 , 陈浩 , 赵聃 , 张胤 , 苏伟明 , 谢乐 , 周小虎 , 周晓洁 . 乳酸菌发酵百香果豆清饮料的动态品质分析:营养价值、抗氧化活性和感官风味[J]. 食品与发酵工业, 2026 , 52(5) : 250 -260 . DOI: 10.13995/j.cnki.11-1802/ts.043221

Abstract

To enhance the utilization of soybean byproduct soy whey, this study developed a co-fermented drink using soy whey supplemented with passion fruit and lactic acid bacteria (LAB) to investigate the dynamic changes in physicochemical indices, viable cell count, nutritional components, antioxidant activity, and flavor compounds during fermentation (0, 6, 12, 18, 24 h).Results showed that the LAB-passion fruit soy whey drink reached the highest total viable count and antioxidant activity at 18 h.Parameters including pH, total acidity, soluble solids, total phenolics, and total flavonoids increased significantly and then stabilized.Compared to pure soy whey fermentation, the conversion rate of isoflavone glucosides to aglycones was slower in the co-fermented beverage.A total of 84 volatile flavor compounds were detected across five fermentation stages, dominated by esters and alcohols, with the highest total content (1 333.30 μg/kg) at 18 h.Partial least squares discriminant analysis and odor activity value analyses identified linalool, hexyl acetate, myrcene, and acetic acid as key marker compounds defining the aroma profile.This study provides theoretical and practical references for soy whey valorization.

参考文献

[1] FEI Y T, LIU L, LIU D M, et al.Investigation on the safety of Lactobacillus amylolyticus L6 and its fermentation properties of tofu whey[J].LWT, 2017, 84:314-322.
[2] CHUA J Y, TAN S J, LIU S Q.The impact of mixed amino acids supplementation on Torulaspora delbrueckii growth and volatile compound modulation in soy whey alcohol fermentation[J].Food Research International, 2021, 140:109901.
[3] FABRICIO M F, SCHMIDT L, ROTHER P D H, et al.Targeted metabolomics of phenolic and volatile compounds during the fermentation of a potential probiotic tofu whey beverage[J].Food Chemistry, 2025, 478:143689.
[4] TU C H, TANG S J, AZI F, et al.Use of kombucha consortium to transform soy whey into a novel functional beverage[J].Journal of Functional Foods, 2019, 52:81-89.
[5] 欧红艳, 赵良忠, 刘汁琪, 等.豆清饮料配方优化及体外模拟消化研究[J].食品与发酵工业, 2021, 47(23):176-184.
OU H Y, ZHAO L Z, LIU Z Q, et al.Formula optimization and simulation digestion in vitro of soybean whey beverage[J].Food and Fermentation Industries, 2021, 47(23):176-184.
[6] TU C H, AZI F, HUANG J, et al.Quality and metagenomic evaluation of a novel functional beverage produced from soy whey using water kefir grains[J].LWT, 2019, 113:108258.
[7] NAZNI P, VAISNAVI G.Formulation and characterization of popsicles using dehydrated passion fruit juice with foxtail millet milk[J].Journal of Food Measurement and Characterization, 2023, 17(3):2083-2094.
[8] NING X, LUO Z H, CHEN Z L, et al.Fortification of set yogurt with passion fruit juice:Effects on fermentation kinetics, physicochemical properties, and functionality[J].Journal of Dairy Science, 2021, 104(4):4084-4093.
[9] 任玲, 田迪, 杨雪怡, 等.发酵前后添加百香果果汁对工夫红茶品质的影响[J].食品工业科技, 2025, 46(11):60-68.
REN L, TIAN D, YANG X Y, et al.Effect of adding passion fruit juice before and after fermentation on the quality of congou black tea[J].Science and Technology of Food Industry, 2025, 46(11):60-68.
[10] ALBUQUERQUE M A C, BEDANI R, LEBLANC J G, et al.Passion fruit by-product and fructooligosaccharides stimulate the growth and folate production by starter and probiotic cultures in fermented soymilk[J].International Journal of Food Microbiology, 2017, 261:35-41.
[11] ZHENG Z J, WEI L Y, ZHU M L, et al.Effect of lactic acid bacteria co-fermentation on antioxidant activity and metabolomic profiles of a juice made from wolfberry and Longan[J].Food Research International, 2023, 174:113547.
[12] 周帆. 高转化大豆异黄酮乳酸菌的筛选及在发酵豆乳中的应用[D].扬州:扬州大学, 2021.
ZHOU F.Screening of lactic acid bacteria with high conversion of soy isoflavones and their application in fermented soy milk[D].Yangzhou:Yangzhou University, 2021.
[13] WANG Y, QI X Y, FU Y, et al.Effects of Torulaspora delbrueckii co-fermented with Saccharomyces cerevisiae on physicochemical and aromatic profiles of blueberry fermented beverage[J].Food Chemistry, 2023, 409:135284.
[14] 吴杰, 陈浩, 张迪, 等.超声波协同漂烫预处理对热风干制凡纳滨对虾品质与风味成分的影响[J].食品与发酵工业,2025,51(23):294-303.
WU J, CHEN H, ZHANG D, et al.Effects of ultrasound-assisted blanching pretreatment on quality and flavor components of hot-air-dried Litopenaeus vannamei[J].Food and Fermentation Industries,2025,51(23):294-303.
[15] 罗静, 李敏, 张莹, 等.电子鼻结合GC-MS分析不同干燥方式对罗非鱼片挥发性风味成分的影响[J].南方水产科学, 2022, 18(1):135-143.
LUO J, LI M, ZHANG Y, et al.Effect of different drying methods on volatile components of tilapia fillets analyzed by electronic nose combined with GC-MS[J].South China Fisheries Science, 2022, 18(1):135-143.
[16] MENG F B, LEI Y T, LI Q Z, et al.Effect of Lactobacillus plantarum and Lactobacillus acidophilus fermentation on antioxidant activity and metabolomic profiles of loquat juice[J].LWT, 2022, 171:114104.
[17] LI T L, JIANG T, LIU N, et al.Biotransformation of phenolic profiles and improvement of antioxidant capacities in jujube juice by select lactic acid bacteria[J].Food Chemistry, 2021, 339:127859.
[18] TIAN Y, SUN L J, YANG Y L, et al.Changes in the physicochemical properties, aromas and polyphenols of not from concentrate (NFC) apple juice during production[J].CyTA-Journal of Food, 2018, 16(1):755-764.
[19] PAN X, ZHANG S, XU X X, et al.Volatile and non-volatile profiles in jujube pulp co-fermented with lactic acid bacteria[J].LWT, 2022, 154:112772.
[20] LI W, WEN L C, CHEN Z T, et al.Study on metabolic variation in whole grains of four proso millet varieties reveals metabolites important for antioxidant properties and quality traits[J].Food Chemistry, 2021, 357:129791.
[21] ZHOU X L, ZHU Z Q, SUN Z H, et al.The fermentation of carambola juice with lactic acid bacteria improves its flavor, bioactive properties, and metabolic composition[J].Food Bioscience, 2025, 66:106307.
[22] DE SOUZA E L, DE ALBUQUERQUE T M R, DOS SANTOS A S, et al.Potential interactions among phenolic compounds and probiotics for mutual boosting of their health-promoting properties and food functionalities-A review[J].Critical Reviews in Food Science and Nutrition, 2019, 59(10):1645-1659.
[23] BELLALOUI N.Soybean seed phenol, lignin, and isoflavones and sugars composition altered by foliar boron application in soybean under water stress[J].Food and Nutrition Sciences, 2012, 3(4):579-590.
[24] 欧红艳, 赵良忠, 李明, 等.豆清饮料发酵过程中大豆异黄酮及风味物质变化规律[J].食品与发酵工业, 2021, 47(24):95-101.
OU H Y, ZHAO L Z, LI M, et al.Changes of soybean isoflavones and flavor substances in soybean whey beverage during fermentation[J].Food and Fermentation Industries, 2021, 47(24):95-101.
[25] HERNÁNDEZ L F, ESPINOSA J C, FERNÁNDEZ-GONZÁLEZ M, et al.β-Glucosidase activity in a Saccharomyces cerevisiae wine strain[J].International Journal of Food Microbiology, 2003, 80(2):171-176.
[26] LI W Y, SONG Y, SUN W S, et al.Both acidic pH value and binding interactions of tartaric acid with α-glucosidase cause the enzyme inhibition:The mechanism in α-glucosidase inhibition of four caffeic and tartaric acid derivates[J].Frontiers in Nutrition, 2021, 8:766756.
[27] LIU Z, HUANG X Z, LIU Q Z, et al.Lactic acid bacteria fermentation improves sensory properties, bioactivity, and metabolic profiles of carrot pulp[J].Food Bioscience, 2025, 66:106303.
[28] LI H C, FAN L J, YANG S Q, et al.Investigation on the nutrient and quality features of jujube juice fermented by selected Lactobacillus acidophilus 6074[J].Food Bioscience, 2024, 57:103496.
[29] JANDROSITZ A, PETSCHNIGG J, ZIMMERMANN R, et al.The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae[J].Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2005, 1735(1):50-58.
[30] 李子晏, 陈颖, 刘瑞玲, 等.发酵时间对蓝莓-胶原蛋白肽饮料功能特性与风味品质的影响[J/OL].食品工业科技, 2025.https://link.cnki.net/doi/10.13386/j.issn1002-0306.2025010273.
LI Z Y, CHEN Y, LIU R L, et al.Effect of fermentation time on functional characteristics and flavor quality of blueberry-collagen peptide beverage[J/OL].Science and Technology of Food Industry, 2025.https://link.cnki.net/doi/10.13386/j.issn1002-0306.2025010273.
文章导航

/

〈 〉