食品与发酵工业 >

2026 , Vol. 52 >Issue 8: 210 - 216

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043824

研究报告

不同酸制备牦牛皮胶原团及胶原纤维膜理化特性

  • 周丽华 ,
  • 罗毅皓 ,
  • 孙万成
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  • (青海大学 农牧学院,青海 西宁,810016)
第一作者:硕士研究生(罗毅皓副教授和孙万成教授为共同通信作者,E-mail:291649347@qq.com;932146238@qq.com)

收稿日期: 2025-07-07

  修回日期: 2025-09-11

  网络出版日期: 2026-05-07

基金资助

青海省科技厅中央引导地方资金(2025ZY006)

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Physicochemical properties of yak skin collagen bundles and collagen fiber films prepared with different acids

  • ZHOU Lihua ,
  • LUO Yihao ,
  • SUN Wancheng
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  • (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

Received date: 2025-07-07

  Revised date: 2025-09-11

  Online published: 2026-05-07

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摘要

胶原纤维膜作为一种可食用薄膜,在香肠加工工业具有重要作用。该研究以盐酸、乙酸作为溶胀酸,以牦牛皮为原料,制备胶原纤维膜,研究酸溶胀对胶原纤维膜理化特性的影响,并通过扫描电镜(scanning electron microscopy,SEM)、傅里叶红外光谱(Fourier infrared spectroscopy,FTIR)、X-衍射(X-diffraction,XRD)、热重分析(thermogravimetric analysis,TGA)对胶原纤维膜中的纤维排列、三螺旋结构及热稳定性进行表征。结果表明,盐酸组具有较强的拉伸强度、较低的含水量、透光度值、热收缩率、溶胀率、热溶胀率;SEM、XRD、TGA和FTIR数据表明牦牛皮胶原纤维在酸溶胀时形成胶原纤维束,盐酸组胶原纤维束更小、更有序,促进分子内和分子间的相互交联,具有更高的三螺旋含量,能够形成更致密的膜结构。盐酸处理通过调控牦牛皮胶原纤维的微观结构,显著提升了胶原纤维膜的综合性能。

关键词： 胶原纤维膜; 胶原团; 盐酸溶胀; 乙酸溶胀; 理化性质

本文引用格式

周丽华 , 罗毅皓 , 孙万成 . 不同酸制备牦牛皮胶原团及胶原纤维膜理化特性[J]. 食品与发酵工业, 2026 , 52(8) : 210 -216 . DOI: 10.13995/j.cnki.11-1802/ts.043824

Abstract

Collagen fiber film has an important role in sausage processing industry as an edible film.It was dedicated to investigate hydrochloric acid and acetic acid as swelling acids to prepare collagen fiber film from yak skin.The effect of acid swelling on the physicochemical properties of collagen fiber film was investigated.The fiber arrangement, triple-helix structure, and thermal stability in the collagen fiber film by scanning electron microscopy (SEM), Fourier infrared spectroscopy (FTIR), X-diffraction (XRD), and thermogravimetric analysis (TGA) was also characterized.The results showed that the hydrochloric acid group had greater tensile strength, lower water content, transmittance value, thermal shrinkage, swelling rate, and thermal swelling rate.SEM, XRD, TGA, and FTIR data indicated that yak skin collagen fibers formed collagen fiber bundles during acid swelling.The collagen fiber bundles in hydrochloric acid group were smaller and more ordered, which promoted intramolecular and intermolecular mutual cross-linking, and possessed higher triple-helix content, and could form a denser membrane structure.The hydrochloric acid treatment significantly improved the comprehensive performance of the collagen fiber membrane by regulating the microstructure of yak skin collagen fibers.

Key words： collagen fiber films; collagen dispersions; hydrochloric acid swelling; acetic acid swelling; physical and chemical properties

参考文献

[1] 田其英, 翟玮玮, 王强.肠衣在香肠中的应用研究进展[J].食品研究与开发, 2022, 43(2):220-224.
TIAN Q Y, ZHAI W W, WANG Q.Research progress on the application of casing in sausage[J].Food Research and Development, 2022, 43(2):220-224.
[2] HOU B Y, WANG B J, WENG Y M.Transglutaminase cross-linked and lysozyme-incorporated antimicrobial tilapia collagen edible films:Development and characterization[J].Foods, 2023, 12(7):1475.
[3] 徐金龙. 胶原纤维可食用膜的机械性能改善策略及相关机制[D].无锡:江南大学, 2021.
XU J L.Improving strategies and related mechanisms for mechanical properties of collagen fiber edible film[D].Wuxi:Jiangnan University, 2021.
[4] LIU Z Q, TUO F Y, SONG L, et al.Action of trypsin on structural changes of collagen fibres from sea cucumber (Stichopus japonicus)[J].Food Chemistry, 2018, 256:113-118.
[5] MA C Y, WANG H T, CHI Y J, et al.Preparation of oriented collagen fiber scaffolds and its application in bone tissue engineering[J].Applied Materials Today, 2021, 22:100902.
[6] RAN R M, XIONG Y M, ZHENG T T, et al.Active and intelligent collagen films containing laccase-catalyzed mulberry extract and Pickering emulsion for fish preservation and freshness indicator[J].Food Hydrocolloids, 2024, 147:109326.
[7] TANG P P, LI X, LI H Y, et al.Development of active film based on collagen and hydroxypropyl methylcellulose incorporating apple polyphenol for food packaging[J].International Journal of Biological Macromolecules, 2024, 273:132960.
[8] LI Y S, YANG J X, SUN L P, et al.Crosslinked fish scale gelatin/alginate dialdehyde functional films incorporated with carbon dots derived from pomelo peel waste for active food packaging[J].International Journal of Biological Macromolecules, 2023, 253(P6):127290.
[9] ANDONEGI M, DE LA CABA K, GUERRERO P.Effect of citric acid on collagen sheets processed by compression[J].Food Hydrocolloids, 2020, 100:105427.
[10] DE GUZZI PLEPIS A M, GOISSIS G, DAS-GUPTA D K.Dielectric and pyroelectric characterization of anionic and native collagen[J].Polymer Engineering & Science, 1996, 36(24):2932-2938.
[11] ZHANG Y H, MA L, CAI L Y, et al.Effects of acid concentration and the UHP pretreatment on the gelatinisation of collagen and the properties of extracted gelatins[J].International Journal of Food Science & Technology, 2016, 51(5):1228-1235.
[12] 吴茜, 刘芳芳, 苗宇, 等.牦牛皮在食品领域的利用现状及应用前景分析[J].食品与发酵工业, 2020, 46(13):305-309.
WU Q, LIU F F, MIAO Y, et al.Analysis of utilization status and application prospect of yak skin in food field[J].Food and Fermentation Industries, 2020, 46(13):305-309.
[13] 李天婵, 张松山, 张志胜, 等.3种牛皮加热过程中食用品质变化规律及其胶原蛋白结构差异性[J].肉类研究, 2020, 34(12):18-23.
LI T C, ZHANG S S, ZHANG Z S, et al.Changes in eating quality and differences in collagen structure of cattle and yak hide during heating[J].Meat Research, 2020, 34(12):18-23.
[14] XU M Q, WEI L X, XIAO Y C, et al.Molecular structural properties of extracted gelatin from yak skin as analysed based on molecular weight[J].International Journal of Food Properties, 2017, 20(sup1):S543-S555.
[15] 王杉杉, 罗学刚, 苏峰丙, 等.牦牛皮胶原蛋白的提取及性能分析[J].精细化工, 2018, 35(5):830-837.
WANG S S, LUO X G, SU F B, et al.Extraction and characterization of collagen from the skin of yak[J].Fine Chemicals, 2018, 35(5):830-837.
[16] 李金, 黄彪, 刘惠考, 等.牦牛胶原蛋白肽降血糖效果研究[J].食品工业科技, 2022, 43(15):338-343.
LI J, HUANG B, LIU H K, et al.Study of yak collagen peptides on reducing blood sugar[J].Science and Technology of Food Industry, 2022, 43(15):338-343.
[17] ZHANG F, LIU H T, PANG X Y, et al.Fabrication and characterization of the bio-based nanofiber solid facial mask with moisturizing and antioxidant efficacy[J].Collagen and Leather, 2025, 7:25.
[18] SUURS P, VAN DEN BRAND H, TEN HAVE R T, et al.Evaluation of cattle skin collagen for producing co-extrusion sausage casing[J].Food Hydrocolloids, 2023, 140:108595.
[19] 徐菲菲, 许健, 刘飞, 等.戊二醛添加顺序对胶原蛋白肠衣膜品质的影响[J].食品与生物技术学报, 2022, 41(12):30-39.
XU F F, XU J, LIU F, et al.Effect of glutaraldehyde addition sequence on quality of collagen casing films[J].Journal of Food Science and Biotechnology, 2022, 41(12):30-39.
[20] CHEN C, LIU F, YU Z, et al.Improvement in physicochemical properties of collagen casings by glutaraldehyde cross-linking and drying temperature regulating[J].Food Chemistry, 2020, 318:126404.
[21] YAN Z H, DOU R R, WEI F, et al.Effects of eugenol on physicochemical properties of sturgeon skin collagen-chitosan composite membrane[J].Journal of Food Science, 2024, 89(7):4032-4046.
[22] XU J L, LIU F, YU Z, et al.Influence of softwood cellulose fiber and chitosan on the film-forming properties of collagen fiber[J].Food Bioscience, 2021, 42:101056.
[23] ZHU K D, YU Z, LI J, et al.Sodium carboxymethyl cellulose as a stabilizer for fabricating mineralized collagen films with improved wet mechanical properties[J].Food Hydrocolloids, 2024, 150:109676.
[24] PARK J W, SCOTT WHITESIDE W, CHO S Y.Mechanical and water vapor barrier properties of extruded and heat-pressed gelatin films[J].LWT-Food Science and Technology, 2008, 41(4):692-700.
[25] GIOSAFATTO C V L, DI PIERRO P, GUNNING P, et al.Characterization of Citrus pectin edible films containing transglutaminase-modified phaseolin[J].Carbohydrate Polymers, 2014, 106:200-208.
[26] ZAREIE Z, TABATABAEI YAZDI F T, ALI MORTAZAVI S A.Development and characterization of antioxidant and antimicrobial edible films based on chitosan and gamma-aminobutyric acid-rich fermented soy protein[J].Carbohydrate Polymers, 2020, 244:116491.
[27] TANG P P, ZHENG T T, YANG C K, et al.Enhanced physicochemical and functional properties of collagen films cross-linked with laccase oxidized phenolic acids for active edible food packaging[J].Food Chemistry, 2022, 393:133353.
[28] WU J M, LIU F, YU Z, et al.Facile preparation of collagen fiber-glycerol-carboxymethyl cellulose composite film by immersing method[J].Carbohydrate Polymers, 2020, 229:115429.
[29] 胡婧瑶, 李丹, 王明月, 等.无机纳米粒子增强大豆蛋白膜功能特性的比较[J].现代食品科技, 2023, 39(10):244-252.
HU J Y, LI D, WANG M Y, et al.Comparison of the functional properties of soy protein isolate films enhanced by inorganic nanoparticles[J].Modern Food Science and Technology, 2023, 39(10):244-252.
[30] 张义. 不同交联剂对胶原蛋白可食膜性能的影响[D].天津:天津科技大学, 2016.
ZHANG Y.Influence of different crosslinking agents on properties of edible collagen film[D], Tianjin:Tianjin University of Science and Technology, 2016.
[31] 胡可欣. 漆酶/没食子酸协同催化交联胶原制备可食用膜的研究[D].成都:四川大学, 2021.
HU K X.Study on preparation of edible membrane by crosslinking collagen catalyzed by laccase/gallic acid[D], Chengdu:Sichuan University, 2021.
[32] WU X M, LIU A J, WANG W H, et al.Improved mechanical properties and thermal-stability of collagen fiber based film by crosslinking with casein, keratin or SPI:Effect of crosslinking process and concentrations of proteins[J].International Journal of Biological Macromolecules, 2018, 109:1319-1328.
[33] MISRA N N, KAUR S, TIWARI B K, et al. Atmospheric pressure cold plasma (ACP) treatment of wheat flour[J].Food Hydrocolloids, 2015, 44: 115-121.
[34] WU X M, LIU Y W, LIU A J, et al.Improved thermal-stability and mechanical properties of type I collagen by crosslinking with casein, keratin and soy protein isolate using transglutaminase[J].International Journal of Biological Macromolecules, 2017, 98:292-301.
[35] WANG W H, ZHANG X L, LI C, et al.Using carboxylated cellulose nanofibers to enhance mechanical and barrier properties of collagen fiber film by electrostatic interaction[J].Journal of the Science of Food and Agriculture, 2018, 98(8):3089-3097.
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