以工厂胀罐酱油为研究对象，>对导致酱油胀罐的产气菌进行分离和鉴定使用高盐 MRS 培养基 ( 含 6 % Na€l) 分离到一种厌氧菌，经产气实验证明该菌是导致酱油产气胀罐的原因,经 16 S rDNA基因测序以及高通量测序确证该厌氧产气菌为破布子乳酸杆菌Lactobacillu?pobusihii) ;采用高盐PDA培养基没有分离到酵母菌,且用多对 18 S rRNA引物对提取的基因组进行扩增阴性，证明酱油胀罐的原因为污染了破布子乳酸菌且没有酵母菌或其他真菌污染，该研究显示了基因测序方法对于食品中未知难培养微生物污染的检测具有优越性

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