食品与发酵工业 >

2026 , Vol. 52 >Issue 12: 11 - 19

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044757

精准发酵

发酵粘液乳杆菌2-1胞外多糖合成与调控机制的全基因组解析

  • 翟江洋 ,
  • 宋慧清 ,
  • 田建军 ,
  • 贺银凤 ,
  • 顾悦
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  • (内蒙古农业大学 食品科学与工程学院,内蒙古 呼和浩特,010018)
第一作者:硕士研究生(顾悦副教授为通信作者,E-mail:gyimau@126.com)

收稿日期: 2025-10-11

  修回日期: 2025-11-20

  网络出版日期: 2026-07-17

基金资助

国家自然科学基金项目(32201996);内蒙古自然科学基金项目(2024MS03063);内蒙古农业大学食品科学与工程学院科技计划项目(SPY2025-5)

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Genomic insights into biosynthesis and regulation of exopolysaccharides in Limosilactobacillus fermentum 2-1

  • ZHAI Jiangyang ,
  • SONG Huiqing ,
  • TIAN Jianjun ,
  • HE Yinfeng ,
  • GU Yue
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  • (College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)

Received date: 2025-10-11

  Revised date: 2025-11-20

  Online published: 2026-07-17

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摘要

胞外多糖(exopolysaccharide, EPS)是乳酸菌重要的代谢产物之一,具有调节菌体功能和改善食品品质等多种作用,广泛应用于食品工业。为解析EPS的合成与调控机制,该研究通过对发酵粘液乳杆菌(Limosilactobacillus fermentum)2-1的全基因组数据进行分析。结果表明:L.fermentum 2-1具有转运纤维二糖、葡萄糖、蔗糖和甘露糖的磷酸烯醇丙酮酸-糖磷酸转移酶系统以及转运乳糖、半乳糖和麦芽糖的渗透酶系统;具有合成UDP-葡萄糖、UDP-半乳糖、dTDP-L-鼠李糖和GDP-甘露糖4种糖核苷酸前体的相关基因;染色体上存在由9个基因组成的eps基因簇,包括转录基因epsA和epsB、链长决定基因epsD、糖基转移酶基因pglC、amsE、tuaG、exoL和wbbL以及EPS转运基因wzx。实时荧光定量PCR结果显示,与胞外多糖合成相关基因在生长阶段均可表达,且epsA、epsD、pglC、exoL、galT和pgm在9 h表达量最高,与此对应EPS产量在9 h达到峰值(857.15 mg/L)。该研究揭示了L.fermentum 2-1中EPS的合成与调控机制,为其在食品工业中的应用提供了理论依据。

关键词： 胞外多糖; 胞外多糖生物合成基因; 生物合成途径; 胞外多糖产量; 实时荧光定量PCR

本文引用格式

翟江洋 , 宋慧清 , 田建军 , 贺银凤 , 顾悦 . 发酵粘液乳杆菌2-1胞外多糖合成与调控机制的全基因组解析[J]. 食品与发酵工业, 2026 , 52(12) : 11 -19 . DOI: 10.13995/j.cnki.11-1802/ts.044757

Abstract

Exopolysaccharides (EPS) are important metabolites synthesized by lactic acid bacteria.They enhance cell protection while improving the texture and stability of fermented foods, thereby offering strong potential for food applications.To elucidate the synthesis and regulation of EPS, the complete genome of Limosilactobacillus fermentum 2-1 was examined.The strain encoded a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS) for the uptake of cellobiose, glucose, sucrose, and mannose, along with permease systems for lactose, galactose, and maltose.Genes involved in generating four sugar-nucleotide precursors—UDP-glucose, UDP-galactose, dTDP-L-rhamnose, and GDP-mannose—were identified.A chromosomal eps cluster with nine genes was annotated, including transcriptional regulators (epsA, epsB), a chain-length determinant (epsD), multiple glycosyltransferases (pglC, amsE, tuaG, exoL, wbbL), and a dedicated polysaccharide transporter (wzx).Real-time quantitative PCR confirmed expression of all eps-associated genes throughout growth.The highest transcription of epsA, epsD, pglC, exoL, galT, and pgm was detected at 9 h, coinciding with the peak EPS concentration of 857.15 mg/L.Overall, these findings indicated that EPS biosynthesis in L.fermentum 2-1 was governed by multiple coordinated genes and strongly linked to carbohydrate import and precursor formation.This study offers a genomic framework for enhancing the functional performance of L.fermentum in food fermentation.

Key words： exopolysaccharides; EPS biosynthetic genes; biosynthetic pathway; EPS production; real-time quantitative PCR

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