食品与发酵工业 >

2026 , Vol. 52 >Issue 12: 70 - 78

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044369

研究报告

人源与牛源乳脂肪球膜蛋白模拟婴儿消化产物的组成与功能差异

  • 张传钰 ,
  • 姜静雅 ,
  • 刘靓 ,
  • 郝欣悦 ,
  • 曾剑华 ,
  • 张兰威 ,
  • 公丕民
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  • 1(中国海洋大学 食品科学与工程学院,山东 青岛,266000)
    2(丹徒区市场监督管理局,江苏 镇江,212000)
    3(安徽科技学院 食品科学与工程学院,安徽 滁州,233100)
第一作者:硕士研究生(张兰威教授和公丕民副教授为共同通信作者,E-mail:zhanglanwei@ouc.edu.cn;gongpimin@ouc.edu.cn)

收稿日期: 2025-08-31

  修回日期: 2025-11-11

  网络出版日期: 2026-07-17

基金资助

国家自然科学基金面上项目(32372289)

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Compositional and functional differences in simulated infant digestive products of human and bovine milk fat globule membrane proteins

  • ZHANG Chuanyu ,
  • JIANG Jingya ,
  • LIU Liang ,
  • HAO Xinyue ,
  • ZENG Jianhua ,
  • ZHANG Lanwei ,
  • GONG Pimin
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  • 1(College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China)
    2(Dantu District Market Supervision and Administration Bureau, Zhenjiang 212000, China)
    3(College of Food Science and Engineering, Anhui Science and Technology University, Chuzhou 233100, China)

Received date: 2025-08-31

  Revised date: 2025-11-11

  Online published: 2026-07-17

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摘要

乳脂肪球膜蛋白(milk fat globule membrane protein, MFGMP)是乳脂肪球膜(milk fat globule membrane, MFGM)中的重要功能性成分,其独特的结构和生物学特性对婴幼儿健康发育具有多种关键作用。该研究利用人和牛源MFGM及其蛋白,探究经体外模拟婴儿胃肠道静态消化各阶段游离氨基酸的变化,运用多肽组学技术分析消化后多肽构成差异,并使用小鼠巨噬细胞RAW264.7模型对不同来源消化产物的抗炎功能进行研究。结果显示,人源MFGMP消化产物中的必需氨基酸(苏氨酸、缬氨酸、异亮氨酸、亮氨酸、赖氨酸)和非必需氨基酸(甘氨酸、丙氨酸、丝氨酸、谷氨酸、天冬氨酸)含量显著高于牛源,且存在6种独有的活性多肽。4种消化产物均无毒性作用且在200、400 μg/mL质量浓度对细胞有一定的促增殖作用,细胞产生NO和免疫因子含量的变化表明人源MFGMP消化产物具有更高的抗炎潜力,MFGM结构对消化产物抗炎作用的影响并不显著。

关键词： 乳脂肪球膜蛋白; 模拟消化; 消化产物; 游离氨基酸; 多肽; 抗炎活性

本文引用格式

张传钰 , 姜静雅 , 刘靓 , 郝欣悦 , 曾剑华 , 张兰威 , 公丕民 . 人源与牛源乳脂肪球膜蛋白模拟婴儿消化产物的组成与功能差异[J]. 食品与发酵工业, 2026 , 52(12) : 70 -78 . DOI: 10.13995/j.cnki.11-1802/ts.044369

Abstract

Milk fat globule membrane protein (MFGMP) is an important functional component of the milk fat globule membrane (MFGM).Its unique structure and biological properties play multiple key roles in infant health and development.This study employed an in vitro static model to simulate the infant gastrointestinal digestion of human and bovine MFGM and its proteins, and monitored dynamic changes in free amino acids during digestion.Peptidomics analysis revealed the differences in the composition of polypeptides.The mouse macrophage RAW264.7 model assessed the anti-inflammatory activity of digestive products from different sources.The results show that human MFGMP digestion products contain significantly higher levels of both essential (Thr, Val, Ile, Leu, Lys) and non-essential (Gly, Ala, Ser, Glu, Asp) amino acids than bovine sources, and contain six unique bioactive peptides.All four digestive products show no cytotoxicity and have certain proliferation-promoting effects on cells at 200 and 400 μg/mL.Measurements of nitric oxide (NO) and immune factors indicate that human MFGMP digestive products possess superior anti-inflammatory potential.Moreover, the MFGM structure shows no significant impact on this anti-inflammatory effect.

Key words： milk fat globule membrane protein; simulated digestion; digestion product; free amino acids; polypeptide; anti-inflammatory activity

参考文献

[1] CLAUMARCHIRANT L, CILLA A, MATENCIO E, et al.Addition of milk fat globule membrane as an ingredient of infant formulas for resembling the polar lipids of human milk[J].International Dairy Journal, 2016, 61:228-238.
[2] HERNELL O, TIMBY N, DOMELLÖF M, et al.Clinical benefits of milk fat globule membranes for infants and children[J].The Journal of Pediatrics, 2016, 173:S60-S65.
[3] GAROFOLI F, CIVARDI E, PISONI C, et al.Anti-inflammatory and anti-allergic properties of colostrum from mothers of full-term and preterm babies:the importance of maternal lactation in the first days[J].Nutrients,2023,15(19):4249.
[4] HOYT A E W, MEDICO T, COMMINS S P.Breast milk and food allergy:Connections and current recommendations[J].Pediatric Clinics of North America, 2015, 62(6):1493-1507.
[5] LANGEL S N, BLASI M, PERMAR S R.Maternal immune protection against infectious diseases[J].Cell Host & Microbe, 2022, 30(5):660-674.
[6] KUBAN K C K, O’SHEA T M, ALLRED E N, et al.The breadth and type of systemic inflammation and the risk of adverse neurological outcomes in extremely low gestation newborns[J].Pediatric Neurology, 2015, 52(1):42-48.
[7] SUN Y, MA S Y, LIU Y B, et al.Changes in interfacial composition and structure of milk fat globules are crucial regulating lipid digestion in simulated in-vitro infant gastrointestinal digestion[J].Food Hydrocolloids, 2023, 134:108003.
[8] STRUIJS K, VAN DE WIELE T, LE T T, et al.Milk fat globule membrane glycoproteins prevent adhesion of the colonic microbiota and result in increased bacterial butyrate production[J].International Dairy Journal, 2013, 32(2):99-109.
[9] LE T T, VAN DE WIELE T, DO T N H, et al.Stability of milk fat globule membrane proteins toward human enzymatic gastrointestinal digestion[J].Journal of Dairy Science, 2012, 95(5):2307-2318.
[10] CAVALETTO M, GIVONETTI A, CATTANEO C.The immunological role of milk fat globule membrane[J].Nutrients, 2022, 14(21):4574.
[11] REN Q X, GUO F, TENG F, et al.A novel approach for the reconstitution of bovine milk fat globules with different-melting-temperature triacylglycerol cores[J].Food Chemistry, 2021, 345:128563.
[12] LI W X, LI M H, CAO X Y, et al.Quantitative proteomic analysis of milk fat globule membrane (MFGM) proteins from donkey colostrum and mature milk[J].Food & Function, 2019, 10(7):4256-4268.
[13] MINEKUS M, ALMINGER M, ALVITO P, et al.A standardised static in vitro digestion method suitable for food–an international consensus[J].Food & Function, 2014, 5(6):1113-1124.
[14] MÉNARD O, BOURLIEU C, DE OLIVEIRA S C, et al.A first step towards a consensus static in vitro model for simulating full-term infant digestion[J].Food Chemistry, 2018, 240:338-345.
[15] LI Z Y, ABOU-ELSOUD M, CHEN H, et al.Identification and molecular mechanism of novel two-way immunomodulatory peptides from ovalbumin:In vitro cell experiments, de novo sequencing, and molecular docking[J].Journal of Agricultural and Food Chemistry, 2024, 72(17):9856-9866.
[16] ZENG J H, LIN K, ZHANG X Q, et al.Insight into the molecular-level details of αs1 casein interactions with IgG:Combining with LC-MS/MS and molecular modelling techniques[J].Food Chemistry, 2023, 399:133987.
[17] ZHENG Z M, HUANG Q L.New insight into the structure-dependent two-way immunomodulatory effects of water-soluble yeast β-glucan in macrophages[J].Carbohydrate Polymers, 2022, 291:119569.
[18] FAN H T, SUN M, LI J, et al.Structure characterization and immunomodulatory activity of a polysaccharide from Saposhnikoviae Radix[J].International Journal of Biological Macromolecules, 2023, 233:123502.
[19] BOUGHTON-SMITH N K, EVANS S M, WHITTLE B J R, et al.Nitric oxide synthase activity in ulcerative colitis and Crohn’s disease[J].The Lancet, 1993, 342(8867):338-340.
[20] LAMPIASI N.Macrophage polarization:Learning to manage it 2.0[J].International Journal of Molecular Sciences, 2023, 24(24):17409.
[21] VAN SADELHOFF J H J, SIZIBA L P, BUCHENAUER L, et al.Free and total amino acids in human milk in relation to maternal and infant characteristics and infant health outcomes:The Ulm SPATZ health study[J].Nutrients, 2021, 13(6):2009.
[22] BOURLIEU C, MÉNARD O, BOUZERZOUR K, et al.Specificity of infant digestive conditions:Some clues for developing relevant in vitro models[J].Critical Reviews in Food Science and Nutrition, 2014, 54(11):1427-1457.
[23] VANDERGHEM C, FRANCIS F, DANTHINE S, et al.Study on the susceptibility of the bovine milk fat globule membrane proteins to enzymatic hydrolysis and organization of some of the proteins[J].International Dairy Journal, 2011, 21(5):312-318.
[24] BURSTAD K M, LAMINA T, ERICKSON A, et al.Evaluation of dietary protein and amino acid requirements:A systematic review[J].The American Journal of Clinical Nutrition, 2025, 122(1):285-305.
[25] NIE C X, HE T, ZHANG W J, et al.Branched chain amino acids:Beyond nutrition metabolism[J].International Journal of Molecular Sciences, 2018, 19(4):954.
[26] CHEN L L, ZHONG Y, OUYANG X Q, et al.Effects of β-alanine on intestinal development and immune performance of weaned piglets[J].Animal Nutrition, 2023, 12:398-408.
[27] DE PALMA G, SHIMBORI C, REED D E, et al.Histamine production by the gut microbiota induces visceral hyperalgesia through histamine 4 receptor signaling in mice[J].Science Translational Medicine, 2022, 14(655):eabj1895.
[28] HOPE H C, SALMOND R J.The role of non-essential amino acids in T cell function and anti-tumour immunity[J].Archivum Immunologiae et Therapiae Experimentalis, 2021, 69(1):29.
[29] LU J, WANG X Y, ZHANG W Q, et al.Comparative proteomics of milk fat globule membrane in different species reveals variations in lactation and nutrition[J].Food Chemistry, 2016, 196:665-672.
[30] WANG B, LI B.Effect of molecular weight on the transepithelial transport and peptidase degradation of casein-derived peptides by using Caco-2 cell model[J].Food Chemistry, 2017, 218:1-8.
[31] HU S, YUAN J L, GAO J Y, et al.Antioxidant and anti-inflammatory potential of peptides derived from in vitro gastrointestinal digestion of germinated and heat-treated foxtail millet (Setaria italica) proteins[J].Journal of Agricultural and Food Chemistry, 2020, 68(35):9415-9426.
[32] YU F M, HE K, DONG X Z, et al.Immunomodulatory activity of low molecular-weight peptides from Nibea japonica skin in cyclophosphamide-induced immunosuppressed mice[J].Journal of Functional Foods, 2020, 68:103888.
[33] VIENNOIS E, PUJADA A, ZEN J, et al.Function, regulation, and pathophysiological relevance of the POT superfamily, specifically PepT1 in inflammatory bowel disease[J].Comprehensive Physiology, 2018, 8(2):731-760.
[34] NING J T, YANG M, ZHU Q, et al.Revealing the diversity of endogenous peptides and parent proteins in human colostrum and mature milk through peptidomics analysis[J].Food Chemistry, 2024, 445:138651.
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