食品与发酵工业 >

2026 , Vol. 52 >Issue 12: 161 - 167

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.044397

研究报告

羟基红花黄色素A对晚期糖基化终末产物形成的抑制作用

  • 王佳敏 ,
  • 张博辉 ,
  • 王来忠 ,
  • 过利敏 ,
  • 包晓玮
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  • 1(新疆农业大学 食品科学与药学学院,新疆 乌鲁木齐,830052)
    2(新疆维吾尔自治区农业科学院 农产品加工研究所,新疆 乌鲁木齐,830091)
    3(新疆宏展特色农业科技开发有限公司,新疆 塔城,834800)
第一作者:硕士研究生(过利敏副研究员和包晓玮教授为共同通信作者,E-mail:guolm_xj@163.com;408531623@qq.com)

收稿日期: 2025-09-03

  修回日期: 2025-10-30

  网络出版日期: 2026-07-17

基金资助

新疆红花综合利用技术研究及产品创制(2023B02005-3);自治区杰出青年基金项目(2024D01E11);国家科技部外国专家引进项目(G2023046003L)

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Inhibitory effect of hydroxysafflor yellow A on formation of advanced glycation end products

  • WANG Jiamin ,
  • ZHANG Bohui ,
  • WANG Laizhong ,
  • GUO Limin ,
  • BAO Xiaowei
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  • 1(School of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China)
    2(Institute of Agro-Products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China)
    3(Xinjiang Hongzhan Characteristic Agricultural Technology Development Co.Ltd., Tacheng 834800, China)

Received date: 2025-09-03

  Revised date: 2025-10-30

  Online published: 2026-07-17

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摘要

该研究旨在探讨羟基红花黄色素A(hydroxysafflor yellow A,HSYA)对晚期糖基化终末产物(advanced glycation end products,AGEs)形成的影响。通过构建牛血清白蛋白(bovine serum protein,BSA)-甲基乙二醛(methylglyoxal,MGO)糖基化模拟体系,结合光谱法、分子对接和SDS-PAGE等方法,检测体系中荧光性AGEs、蛋白质氧化产物(二酪氨酸、犬尿氨酸、N-甲酰犬尿氨酸),并研究HSYA与BSA的结合特征。结果表明,300 μmol/L的HSYA对荧光性AGEs、二酪氨酸、犬尿氨酸及N-甲酰犬尿氨酸的抑制率分别达到70.07%、51.71%、68.34%和80.37%。荧光光谱结果表明,HSYA与BSA之间有1类结合位点,猝灭机制为静态猝灭,且二者之间主要作用力为氢键和范德华力。SDS-PAGE结果表明,HSYA能够抑制BSA-MGO体系中BSA交联结构的形成。分子对接结果表明,HSYA可结合到BSA的TRP-213残基与BSA形成复合物,同时与BSA的ARG残基之间存在氢键和范德华力,阻碍BSA糖基化位点与MGO结合,从而抑制AGEs的产生。因此,HSYA具有开发为AGEs天然抑制剂的潜力。

关键词： 羟基红花黄色素A; 晚期糖基化终末产物; 荧光光谱; 分子对接

本文引用格式

王佳敏 , 张博辉 , 王来忠 , 过利敏 , 包晓玮 . 羟基红花黄色素A对晚期糖基化终末产物形成的抑制作用[J]. 食品与发酵工业, 2026 , 52(12) : 161 -167 . DOI: 10.13995/j.cnki.11-1802/ts.044397

Abstract

This study aimed to explore the effect of hydroxysafflor yellow A (HSYA) on the formation of advanced glycation end products (AGEs).By establishing a simulated glycation system using bovine serum protein (BSA) and methylglyoxal (MGO), combined with spectroscopic analysis, molecular docking, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), this study quantitatively measured fluorescent AGEs, protein oxidation products (dityrosine, kynurenine, N-formylkynurenine) in the system to investigate the binding characteristics of HSYA with BSA.Results showed that HSYA at 300 μmol/L achieved inhibition rates of 70.07%, 51.71%, 68.34% and 80.37% on fluorescent AGEs, dityrosine, kynurenine and N-formylkynurenine, respectively.Fluorescence spectroscopy results showed that the existence of a binding site between HSYA and BSA.The quenching mechanism was static quenching and the main forces were hydrogen bonds and van der Waals forces.SDS-PAGE results showed that HSYA could inhibit the formation of BSA cross-linked structures in the BSA-MGO system.Molecular docking results showed that HSYA can bind to the TRP-213 residue of BSA to form a complex with BSA, and at the same time there is a hydrogen bond and van der Waals force between the ARG residue of BSA, which hinders the binding of BSA glycosylation sites to MGO, thereby inhibiting the production of AGEs.Therefore, HSYA has the potential to be developed as a natural inhibitor of AGEs.

Key words： hydroxysafflor yellow A; advanced glycation end products; fluorescence spectroscopy; molecular docking

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