鲨鱼软骨作为海洋生物肽的重要来源,具有多种生物活性,但关于大吻斜齿鲨(Scoliodon macrorhynchos)软骨肽(shark cartilage peptides,SCPs)结构及生物功能研究较少,该文通过LC-MS/MS对SCPs的肽段序列进行鉴定,并对多种生物活性进行预测。得到354条质量偏差控制较好的多肽,其中四肽177条,五肽104条,分子质量集中在400~500 Da和500~600 Da,Leu(386)、Ile(301)和Pro(205)数量最多,N端和C端氨基酸以非极性脂肪族氨基酸、极性脂肪族氨基酸和芳香族氨基酸为主,肽段整体具有弱酸性、弱负电性,疏水性和芳香性。经预测具有血管紧张素转化酶抑制性、糖苷酶抑制性、抗癌、鲜味、抗炎、抗氧化等12种活性,含有抗炎、抗氧化、降血压的多肽段最多,其中62条多肽序列在活性肽数据库BIOPEP-UWM报道。研究结果可为多肽合成提供依据。
Shark cartilage, an important source of marine bioactive peptides, has a variety of bioactivities, but there are fewer studies on the structure and biofunction of the cartilage peptides (shark cartilage peptides, SCPs) of Scoliodon macrorhynchos. The peptide sequences of SCPs were characterized by LC-MS/MS, and a series of biological activities were predicted accordingly.We obtained 354 peptides with good quality deviation control, including 177 tetrapeptides and 104 pentapeptides, with molecular weights concentrated in 400-500 Da and 500-600 Da, Leu (386), Ile (301), and Pro (205) were the most abundant residues, and the N- terminal and C-terminal residues were predominantly nonpolar aliphatic, polar aliphatic, and aromatic amino acids.The peptides exhibited overall weak acidity, a slight negative charge, hydrophobicity, and aromaticity.Bioinformatic analysis predicted 12 bioactive functions, including angiotensin-converting enzyme inhibition, glycosidase inhibition, anticancer, umami, anti-inflammatory, and antioxidant activities, peptides with anti-inflammatory, antioxidant, and antihypertensive properties were the most abundant, among these, 62 peptide sequences are reported in the BIOPEP-UWM database.This work provides a basis for peptide synthesis.
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