为了比较康宁木霉(Trichoderma koningii 3.2774)/产朊假丝酵母(Candida utilis 2.281)混菌发酵和康宁木霉(Trichoderma koningii 3.2774)单菌发酵对固态发酵三七渣生产单细胞蛋白的影响,该研究考察了发酵过程中粗纤维含量、真蛋白含量、还原糖含量、纤维素酶活力随时间的变化,并建立了粗纤维降解动力学、菌体生长动力学和真蛋白生成动力学模型。研究结果表明,在混菌与单菌发酵体系中真蛋白的最大含量分别为18.47%和16.76%。粗纤维降解动力学适合采用Logistic模型进行拟合,在混菌与单菌发酵体系中其降解速率分别在发酵第29.26 h和38.85 h达到峰值,其数值分别为0.178 5%/h和0.107 3%/h;菌体生长动力学适合采用SGompertz模型进行拟合,在混菌与单菌发酵体系中菌体的最大比生长速率分别为0.038 70 h-1和0.027 72 h-1,菌体生长速率分别在发酵第55.98 h和62.11 h达到峰值,其数值分别为0.121 8%/h和0.072 89%/h;真蛋白生成动力学适合采用Logistic模型进行拟合,在混菌与单菌发酵体系中真蛋白的生成速率分别在发酵第55.83 h和63.76 h 达到峰值,其数值分别为0.124 5%/h、0.076 24%/h。与康宁木霉单菌发酵相比,康宁木霉/产朊假丝酵母混菌发酵有助于消除还原糖的反馈抑制作用,对三七渣中粗纤维的降解能力更强,发酵培养物中真蛋白含量更高。
To compare the effects of mixed-culture fermentation with Trichoderma koningii 3.2774 and Candida utilis 2.281 versus mono-culture fermentation with Trichoderma koningii 3.2774 on the production of single-cell protein from Panax notoginseng residue via solid-state fermentation, this study investigated the temporal changes in crude fiber content, true protein content, reducing sugar content, and cellulase activity.Furthermore, kinetic models for crude fiber degradation, microbial growth, and true protein production were established.Results indicated that the maximum true protein content in the mixed-culture and single-strain fermentation systems reached 18.47% and 16.76%, respectively.The crude fiber degradation kinetics were well fitted by the Logistic model, with the degradation rates peaking at 29.26 h and 38.85 h of fermentation, reaching values of 0.178 5%/h and 0.107 3%/h, respectively.Microbial growth kinetics were appropriately described by the SGompertz model, showing maximum specific growth rates of 0.038 70 h-1 and 0.027 72 h-1 in the mixed-culture and single-strain systems, respectively.The growth rates peaked at 55.98 h and 62.11 h of fermentation, with values of 0.121 8%/h and 0.072 89%/h, respectively.True protein synthesis kinetics followed the Logistic model, with synthesis rates peaking at 55.83 h and 63.76 h of fermentation, reaching 0.124 5%/h and 0.076 24%/h, respectively.Compared with single-strain fermentation by Trichoderma koningii 3.2774, the mixed-culture fermentation with Trichoderma koningii 3.2774 and Candida utilis 2.281 effectively alleviated the feedback inhibition caused by reducing sugars, enhanced the degradation capacity of crude fiber in Panax notoginseng residue, and resulted in higher true protein content in the fermented product.
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