该文探究不同采收期樟头红陈皮的化学成分差异,阐释其功效物质基础,为樟头红陈皮商品规格的区分及临床应用提供科学依据。利用LC-MS结合多元统计分析对不同采收期樟头红陈皮进行主成分分析及偏最小二乘-判别分析,寻找不同采收期樟头红陈皮差异成分,对差异成分进行聚类热图分析、箱线图分析和相关性分析。可以将不同采收期的樟头红陈皮分成微红皮和大红皮两大类,筛选出25种差异成分,其中黄酮类成分18种。黄柏酮、黄杞苷、圣草次苷和柚皮苷在大红皮中的含量显著高于微红皮;槲皮素-3,7,3′,4′-四甲醚、栀子黄素B、3,5,6,7,8,3′,4′-七甲氧基黄酮、异甘草苷、5,6,7,4′-四甲氧基黄酮、苜蓿素和女贞苷在微红皮中的含量显著高于大红皮。枸橘苷、枸橼苦素、黄柏酮、花侧柏烯的含量变化最大,最高值与最低值相差5~20倍。该方法能够快速、准确地对不同采收期樟头红陈皮的化学成分进行全面分析,从化学成分差异方面阐释微红皮及大红皮的生物活性差异的物质基础,为樟头红陈皮这一江西特色品种商品规格的确定提供科学依据,并为柑橘属植物的采收期的确定提供良好的借鉴。
To explore the differences in the chemical composition of Citri Reticulatae Pericarpium at different harvest stages, to explain its efficacy material basis, and to provide a scientific basis for the differentiation and clinical application of Citri Reticulatae Pericarpium commodity specifications.Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were carried out on Citri Reticulatae Pericarpium at different harvest stages by LC-MS combined with multivariate statistical analysis.The Citri Reticulatae Pericarpium at different harvest stages could be divided into two categories, namely weihongpi and dahongpi, and 25 different components were screened out, including 18 flavonoids.The contents of obacunone, engeletin, eriocitrin, and naringin in the Dahongpi were significantly higher than those in the Weihongpi.The contents of quercetin-3,7,3′,4′-tetramethyl ether, gardenia b, 3,5,6,7,8,3′,4′-heptamethoxyflavone, isoliquiritin, scutellarein tetramethyl ether, tricin and ligustroflavone in weihongpi were significantly higher than those in dahongpi.The contents of poncirin, 7-[(3,3-dimethyloxiran-2-yl)methoxy]-8-[(3,3-dimethyloxiran-2-yl)methyl]-2H-1-benzopyran-2-one, obacunone, and cuparene changed the most, with a difference of 5 to 20 times between the highest and lowest values.This method can quickly and accurately analyze the chemical composition of Citri Reticulatae Pericarpium at different harvest stages, and explain the material basis of the biological activity differences between weihongpi and dahongpi from the perspective of chemical composition, which provides a scientific basis for the determination of the commodity specifications of Citri Reticulatae Pericarpium, a characteristic variety in Jiangxi Province, and provides a good reference for the determination of the harvest period of citrus plants.
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