研究报告

美拉德反应对甜瓜螺肉水解物的理化性质和风味特性的影响

  • 吕思盈 ,
  • 蒋蝉聪 ,
  • 杨耀冲 ,
  • 邱伟强 ,
  • 包建强 ,
  • 金银哲
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(上海水产品加工及贮藏工程技术研究中心,上海,201306)
    3(农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海,201306)
    4(食品热加工工程中心,上海,201306)
第一作者:硕士研究生(金银哲副教授为通信作者,E-mail:yzjin@shou.edu.cn)

收稿日期: 2025-12-08

  修回日期: 2026-01-04

  网络出版日期: 2026-09-21

基金资助

国家重点研发计划项目(2020YFD0900905)

Effect of the Maillard reaction on the physicochemical properties and flavor characteristics of Cymbium pepo hydrolysate

  • LYU Siying ,
  • JIANG Chancong ,
  • YANG Yaochong ,
  • QIU Weiqiang ,
  • BAO Jianqiang ,
  • JIN Yinzhe
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  • 1(College of Food Science, Shanghai Ocean University, Shanghai 201306, China)
    2(Shanghai Engineering Research Center for Processing and Storage of Aquatic Products, Shanghai 201306, China)
    3(Laboratory of Quality and Safety Risk Assessment of Aquatic Products Storage and Preservation, Ministry of Agriculture (Shanghai), Shanghai 201306, China)
    4(Engineering Research Center of Food Thermal-Processing Technology, Shanghai 201306, China)

Received date: 2025-12-08

  Revised date: 2026-01-04

  Online published: 2026-09-21

摘要

美拉德反应在食品加工过程中至关重要,对改善食品风味起着积极作用。该研究旨在探讨美拉德反应对甜瓜螺肉水解物的理化性质和风味特性的影响,以甜瓜螺肉水解物和4种还原糖为原料,探究美拉德反应产物的中间产物、褐变程度、接枝度和游离氨基酸等理化性质的变化,同时,结合气相色谱-离子迁移谱(gas chromatography-ion mobility spectrometry, GC-IMS)、电子舌和感官评价分析美拉德反应产物的风味和滋味变化。结果表明,经过美拉德反应后,反应产物的中间产物、褐变程度、接枝度和抗氧化性显著提升,降低了水解物的苦味氨基酸,改善了水解物的颜色和风味特性,同时电子舌能有效区分不同反应产物的滋味差异。通过GC-IMS鉴定出水解物和美拉德反应产物具有38种挥发性化合物,美拉德反应产物产生了更多的吡嗪和呋喃等物质,减少了醛类化合物,从而增强了烤肉和坚果香味,减小了腥味等令人不快的风味。该研究为甜瓜螺肉水解物的风味改善提供了新思路,对风味产品的开发提供理论参考。

本文引用格式

吕思盈 , 蒋蝉聪 , 杨耀冲 , 邱伟强 , 包建强 , 金银哲 . 美拉德反应对甜瓜螺肉水解物的理化性质和风味特性的影响[J]. 食品与发酵工业, 2026 , 52(17) : 282 -290 . DOI: 10.13995/j.cnki.11-1802/ts.045421

Abstract

The Maillard reaction plays a crucial role in food processing and contributes positively to enhancing food flavor.This study aims to explore the effects of the Maillard reaction on the physicochemical properties and flavor characteristics of Cymbium pepo hydrolysate.Using C.pepo hydrolysate and four reducing sugars as raw materials.Investigate the changes in the physicochemical properties of the Maillard reaction products, including intermediate products, browning intensity, the degree of graft, and free amino acids.Meanwhile, the flavor and taste changes of Maillard reaction products were analyzed using gas chromatography-ion mobility spectrometry (GC-IMS), electronic tongue, and sensory evaluation.The results showed that after the Maillard reaction, the intermediate products, browning intensity, the degree of graft, and antioxidant activities of the reaction products significantly increased.The bitter amino acids in the hydrolysate were reduced, and the color and flavor characteristics of the hydrolysate were improved.Meanwhile, the electronic tongue can effectively distinguish the taste differences of various reaction products.A total of 38 volatile compounds were identified in the hydrolysate and Maillard reaction products by GC-IMS.The Maillard reaction products generated more substances such as pyrazines and furans, reducing aldehyde compounds, thereby enhancing the aroma of roasted meat and nuts, and diminishing unpleasant flavors such as fishiness.This study provided novel insights into enhancing the flavor of C.pepo hydrolysate, providing a theoretical basis for the development of flavored products.

参考文献

[1] Li G S, Hu L P, Hu Z H, et al.Nutrition and protein function, properties (structure, rheology, thermal stability) analysis of nepture volute based on proteomics and in vitro digestion/cells model[J].Food Bioscience, 2021, 43:101321.
[2] 钟机. 蓝圆鲹鱼肉蛋白酶解液的制备及其Maillard反应生香工艺研究[D].福州:福建农林大学, 2017.
Zhong J.Study on the preparation of enzymatic hydrolysate of Decapterus maruadsi proteins and Maillard reaction[D].Fuzhou:Fujian Agriculture and Forestry University, 2017.
[3] Liu L, Zhao Y H, Lu S X, et al.Metabolomics investigation on the volatile and non-volatile composition in enzymatic hydrolysates of Pacific oyster (Crassostrea gigas)[J].Food Chemistry:X, 2023, 17:100569.
[4] 张亦鸣. 低聚木糖美拉德反应及其衍生物活性[D].上海:上海海洋大学, 2015.
Zhang Y M.The Maillard reaction of xylo-oligosaccharides and the biological activity of its derivatives[D].Shanghai:Shanghai Ocean University, 2015.
[5] 秦晓辉. 美拉德反应制备珠蚌抗氧化肽及其性质研究[D].南昌:南昌大学, 2014.
Qin X H.Research on the preparation and antioxidant of peptide from mussels by Maillard reaction[D].Nanchang:Nanchang University, 2014.
[6] Long Z S, Yi X Z, Gao X, et al.Combining sensory analysis and flavoromics to determine how the Maillard reaction affects the flavors of golden pomfret hydrolysates[J].Foods, 2025, 14(4):560.
[7] Qiu D, Gan R Q, Feng Q H, et al.Flavor formation of tilapia byproduct hydrolysates in Maillard reaction[J].Journal of Food Science, 2024, 89(3):1554-1566.
[8] Zhang X, Liu S Q.Effects of reducing sugars on colour, amino acids, and volatile flavour compounds in thermally treated minced chicken carcass hydrolysate[J].Foods, 2024, 13(7):991.
[9] Zhao Z L, Wang W D, Chen J, et al.Effect of ultrasound-assisted Maillard reaction on functional properties and flavor characteristics of Oyster protein enzymatic hydrolysates[J].Ultrasonics Sonochemistry, 2024, 111:107113.
[10] Lyu Y N, Bai X T, Zhao H L, et al.Flavor characteristics of large yellow croaker soup served with different dried edible fungi[J].Food chemistry:X, 2024, 21:101059.
[11] 王玉婷, 肖乃勇, 施文正.超声辅助美拉德反应对鲢鱼酶解产物风味特性的影响[J].渔业现代化, 2023, 50(6):84-92.
Wang Y T, Xiao N Y, Shi W Z.Effect of ultrasound-assisted Maillard reaction on the flavor characteristics of hydrolysate of Hypophthalmichthys molitrix[J].Fishery Modernization, 2023, 50(6):84-92.
[12] 栗紫慧. 黑鱼加工过程中风味与品质变化的研究[D].上海:上海海洋大学, 2024.
Li Z H.Research on changes in flavor and quality of snakehead fish during processing[D].Shanghai:Shanghai Ocean University, 2024.
[13] 王艳茹, 王欣悦, 何龙, 等.超高压辅助酶解制备胶原蛋白抗氧化肽及构效分析[J].食品科学, 2026,47(3):109-119.
Wang Y R, Wang X Y, HE L, et al.Preparation of collagen antioxidant peptides by ultrahigh-pressure-assisted enzymatic digestion and constitutive analysis[J].Food Science, 2026,47(3):109-119.
[14] Gao J J, Ning C, Wang M X, et al.Structural, antioxidant activity, and stability studies of jellyfish collagen peptide-calcium chelates[J].Food Chemistry:X, 2024, 23:101706.
[15] Yang X, Li Y L, Li S T, et al.Effects and mechanism of ultrasound pretreatment of protein on the Maillard reaction of protein-hydrolysate from grass carp (Ctenopharyngodon idella)[J].Ultrasonics Sonochemistry, 2020, 64:104964.
[16] 陈晓. 鸡肝蛋白酶解物的美拉德反应条件优化及其产物特性[D].合肥:安徽农业大学, 2020.
Chen X.Optimization of Maillard reaction conditions for chicken liver protein enzymatic hydrolysate and its product characteristics[D].Hefei:Anhui Agriculture University, 2020.
[17] 张玲, 罗嘉滨, 海金萍, 等.海蜇皮酶解液美拉德反应制备鱼味香料基液的研究[J].中国调味品, 2012, 37(1):28-31;44.
Zhang L, Luo J B, Hai J P, et al.Study on the preparation of fish flavor by Maillard reaction on enzymatic hydrolyzate of jellyfish[J].China Condiment, 2012, 37(1):28-31;44.
[18] 葛伟. 超声预处理对酪蛋白美拉德反应及其产物功能特性的影响[D].哈尔滨:东北农业大学, 2015.
Ge W.The effect of ultrasonic pretreatment on Maillard reaction and functional properties of Maillard reaction products of casein[D].Harbin:Northeast Agricultural University, 2015.
[19] Liu Z H, Chen B, Wang S L, et al.Maillard reaction products from tilapia (Oreochromis mossambicus) scale collagen peptides conjugated with galactooligosaccharides of different purities:Characterization, antioxidant activity, and impact on the growth of probiotics[J].Food Chemistry:X, 2025, 29:102733.
[20] Sun L B, Wang D H, Huang Z, et al.Structure and flavor characteristics of Maillard reaction products derived from soybean meal hydrolysates-reducing sugars[J].LWT, 2023, 185:115097.
[21] Sun A, Chen L N, Wu W, et al.The potential meat flavoring generated from Maillard reaction products of wheat gluten protein hydrolysates-xylose:Impacts of different thermal treatment temperatures on flavor[J].Food Research International, 2023, 165:112512.
[22] Nishimura K, Abe T.Effect of protease reaction conditions on volatile compounds generated in Maillard reaction products from soy protein hydrolysates[J].Food Chemistry, 2025, 464:141599.
[23] Hao J Q, Zhang X Y, Wang Z W, et al.Maillard reaction products of soybean protein hydrolysates and reducing sugar:Structure and flavor insights[J].Food Research International, 2025, 202:115790.
[24] 王梦云, 杜婕妤, 桑尚源, 等.5种还原糖与南极磷虾粉酶解液美拉德反应产物挥发性成分的差异分析[J].宁波大学学报(理工版), 2022, 35(4):15-28.
Wang M Y, Du J Y, Sang S Y, et al.The difference analysis of volatile components in Maillard reaction products prepared from hydrolysate of Antarctic krill powder and five kinds of reducing sugar[J].Journal of Ningbo University:Natural Science and Engineering Edition, 2022, 35(4):15-28.
[25] 李子晏, 陈颖, 刘瑞玲, 等.发酵时间对蓝莓-胶原蛋白肽饮料功能特性与风味品质的影响[J].食品工业科技, 2026,47(3):124-137.
Li Z Y, Chen Y, Liu R L, et al.Effect of fermentation time on the functional properties and flavor quality of blueberry-collagen peptide fermented beverages[J].Science and Technology of Food Industry, 2026,47(3):124-137.
[26] 张奇秀. 鱿鱼鱼糜凝胶品质比较及其混合鱼糜研究[D].上海:上海海洋大学, 2023.
Zhang Q X.Study on comparison of gel quality of squid surimi and its mixed surimi[D].Shanghai:Shanghai Ocean University, 2023.
[27] Han J R, Du Y N, Tang Y, et al.Structural changes, volatile compounds and antioxidant activities of Maillard reaction products derived from scallop (Patinopecten yessoensis) female gonad hydrolysates[J].Journal of Aquatic Food Product Technology, 2019, 28(4):352-364.
[28] 林阳, 张馨月, 李晓磊, 等.基于电子鼻和离子迁移谱分析三种烤鱼片挥发性风味化合物[J].食品工业科技, 2025, 46(24):291-301.
Lin Y, Zhang X Y, Li X L, et al.Analysis of volatile flavor compounds in three types of baked fish fillets using an electronic nose with GC-IMS[J].Science and Technology of Food Industry, 2025, 46(24):291-301.
[29] Huang A Q, Liu Q, Chen X T, et al.Preparation and characterization of Maillard reaction products from enzymatic hydrolysate products of Abalone viscera[J].Microchemical Journal, 2024, 204:111062.
[30] 张美. 刺参性腺酶解物及其美拉德反应产物风味和抗氧化活性研究[D].上海:上海海洋大学, 2023.
Zhang M.Study of flavor and antioxidant activity in stichopus japonisus gonads enzymatics and Maillard reaction products[D].Shanghai:Shanghai Ocean University, 2023.
[31] Sharma K, Nilsuwan K, Hong H, et al.Debittering of salmon frame protein hydrolysate and plastein using Maillard reaction as affected by types of sugar[J].International Journal of Food Science and Technology, 2024, 59(3):1560-1571.
[32] 陈晴. 糖基化对鲐鱼鱼松品质及体外消化特性的影响[D].上海:上海海洋大学, 2022.
Chen Q.Effects of glycosylation on the quality and in vitro digestion characteristics of mackerel (Scomber japonicus) fish floss[D].Shanghai:Shanghai Ocean University, 2022.
[33] Chen K N, Yang Q F, Hong H, et al.Physicochemical and functional properties of Maillard reaction products derived from cod (Gadus morhua L.) skin collagen peptides and xylose[J].Food Chemistry, 2020, 333:127489.
[34] Zhang X G, Noisa P, Hamzeh A, et al.Glycation of tilapia protein hydrolysate decreases cellular antioxidant activity upon in vitro gastrointestinal digestion[J].Food Chemistry:X, 2024, 21:101228.
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